A5022465137- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- Molecular Sensors and Ion Detection
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Cardiac electrophysiology and arrhythmias
- Bacteriophages and microbial interactions
- Toxoplasma gondii Research Studies
- Viral Infections and Vectors
- Pharmaceutical and Antibiotic Environmental Impacts
- Electrochemical sensors and biosensors
- Ion channel regulation and function
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Research and Treatments
- Cardiovascular Effects of Exercise
- COVID-19 epidemiological studies
- Antibiotic Resistance in Bacteria
- Handwritten Text Recognition Techniques
- Respiratory viral infections research
- Advanced Biosensing Techniques and Applications
- Network Security and Intrusion Detection
- RNA Interference and Gene Delivery
- Zoonotic diseases and public health
Shanghai Jiao Tong University
2021-2025
University of Electronic Science and Technology of China
2025
Tencent (China)
2023-2024
Second Military Medical University
2023
Shandong First Medical University
2015-2023
Changhai Hospital
2023
Chinese People's Armed Police Force Engineering University
2021-2022
Sun Yat-sen University
2016-2022
UConn Health
2018-2021
Farmington Community Library
2019-2021
The recent outbreak of novel coronavirus (SARS-CoV-2) causing COVID-19 disease spreads rapidly in the world. Rapid and early detection SARS-CoV-2 facilitates intervention prevents spread. Here, we present an All-In-One Dual CRISPR-Cas12a (AIOD-CRISPR) assay for one-pot, ultrasensitive, visual detection. By targeting SARS-CoV-2's nucleoprotein gene, two CRISPR RNAs without protospacer adjacent motif (PAM) site limitation are introduced to develop AIOD-CRISPR detect nucleic acids with a...
Recently, CRISPR-Cas technology has opened a new era of nucleic acid-based molecular diagnostics. However, current CRISPR-Cas-based acid biosensing lack the quantitative detection ability and typically requires separate manual operations. Herein, we reported dynamic aqueous multiphase reaction (DAMR) system for simple, sensitive one-pot CRISPR-Cas12a based diagnosis by taking advantage density difference sucrose concentration. In DAMR system, recombinase polymerase amplification (RPA)...
Abstract A recent outbreak of novel coronavirus (SARS-CoV-2), the causative agent COVID-19, has spread rapidly all over world. Human immunodeficiency virus (HIV) is another deadly and causes acquired syndrome (AIDS). Rapid early detection these viruses will facilitate intervention reduce disease transmission risk. Here, we present an ll- I n- O ne D ual CRISPR -Cas12a (termed “AIOD-CRISPR”) assay method for simple, rapid, ultrasensitive, one-pot, visual SARS-CoV-2 HIV virus. In our AIOD...
The COVID-19 pandemic, caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), has become a public health emergency and widely spread around the world. Rapid, accurate early diagnosis of infection plays crucial role in breaking this pandemic. However, detection accuracy is limited for current single-gene SARS-CoV-2. Herein, we develop an autonomous lab-on-paper platform multiplex gene SARS-CoV-2 combining reverse transcription recombinase polymerase amplification (RT-RPA)...
Rationale:Early and accurate detection of disease is crucial for its prevention, identification, treatment.However, most diagnostics still limited in clinical laboratories due to the need complicated instruments professional personnel.Herein, we reported a smartphone-based synergistically enhanced colorimetric method molecular our point care (POC) smart cup platform.Methods: A disposable microfluidic chip was developed loop-mediated isothermal amplification (LAMP) multiple HPV DNA POC...
Abstract Rapid diagnostics of infectious diseases and accurate identification their causative pathogens play a crucial role in disease prevention, monitoring, treatment. Conventional molecular detection requires expensive equipment well-trained personnel, thus limiting its use centralized clinical laboratories. To address this challenge, portable smartphone-based quantitative platform, termed “smart connected pathogen tracer” (SCPT), has been developed for monitoring surveillance. The...
CRISPR/Cas-based diagnostics offer unparalleled specificity, but their reliance on fluorescently labeled probes and complex nucleic acid extraction limits field applicability. To tackle this problem, we have developed a label-free, equipment-free platform integrating FTA card-based extraction, CRISPR/Cas12a, pre-folded G-quadruplex (G4)–Thioflavin T (ThT) signal reporter. This system eliminates costly fluorescent labeling by leveraging G4-ThT structural binding for visible fluorescence...