A5040561460- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- RNA Research and Splicing
- Prostate Cancer Treatment and Research
- Single-cell and spatial transcriptomics
- Peptidase Inhibition and Analysis
- Cancer-related molecular mechanisms research
- Genetics and Neurodevelopmental Disorders
- Tissue Engineering and Regenerative Medicine
- Phagocytosis and Immune Regulation
- Macrophage Migration Inhibitory Factor
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Chemokine receptors and signaling
- Histone Deacetylase Inhibitors Research
- Nerve injury and regeneration
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Cellular transport and secretion
- interferon and immune responses
Peking University
2022-2025
Boston Children's Hospital
2021-2024
Harvard University
2021-2024
China University of Petroleum, East China
2022-2024
Texas A&M University
2024
King University
2024
China Energy Engineering Corporation (China)
2024
Jilin University
2010-2023
Houston Methodist
1990-2023
Peking University International Hospital
2022-2023
Lipid incorporation from endoplasmic reticulum (ER) to lipid droplet (LD) is important in controlling LD growth and intracellular homeostasis. However, the molecular link mediating ER cross talk remains elusive. Here, we identified Rab18 as an Rab guanosine triphosphatase maturation. deficiency resulted a drastically reduced number of mature LDs decreased storage, was accompanied by increased stress. Rab3GAP1/2, GEF Rab18, promoted activating targeting LDs. LD-associated bound specifically...
Genome-wide analyses of changes in gene expression, transcription factor occupancy on DNA, histone modification patterns chromatin, genomic copy number variation, and nucleosome positioning have become popular many modern laboratories, yielding a wealth information during health disease states. However, most these studies overlooked an inherent normalization problem that must be corrected with spike-in controls. Here we describe the reason why controls are so important explain how to...
Abstract Reprogrammed cellular metabolism is essential for maintaining cancer stem cells (CSCs) state. Here, we report that mitochondrial D-lactate catabolism a necessary initiating oncogenic event during tumorigenesis of esophageal squamous cell carcinoma (ESCC). We discover cyclin-dependent kinase 7 (CDK7) phosphorylates nuclear Yes-associated protein 1 (YAP) at S127 and S397 sites enhances its transcription function, which promotes dehydrogenase (LDHD) expression. Moreover, LDHD enriched...
BMI1, a polycomb group (PcG) protein, plays critical role in epigenetic regulation of cell differentiation and proliferation, cancer stem self-renewal. BMI1 is upregulated multiple types cancer, including prostate cancer. As key component repressive complex 1 (PRC1), exerts its oncogenic functions by enhancing the enzymatic activities RING1B to ubiquitinate histone H2A at lysine 119 repress gene transcription. Here, we report PRC1-independent that for castration-resistant (CRPC) progression....
Polycomb group proteins are important epigenetic regulators for cell proliferation and differentiation, organ development, as well initiation progression of lethal diseases, including cancer. Upregulated proteins, Enhancer zeste homolog 2 (EZH2), promote proliferation, migration, invasion metastasis cancer cells, self‐renewal stem cells. In our study, we report that EZH2 embryonic ectoderm development (EED) indicate respective direct interaction with androgen receptor (AR). the context...
A comprehensive understanding of endothelial cell lineage specification will advance cardiovascular regenerative medicine. Recent studies found that unique epigenetic signatures preferentially regulate identity genes. We thus systematically investigate the landscape and identify MECOM to be leading candidate as an regulator. Single-cell RNA-Seq analysis verifies MECOM-positive cells are exclusively enriched in cluster bona fide derived from induced pluripotent stem cells. Our experiments...
Cell identity genes are distinct from other with respect to the epigenetic mechanisms activate their transcription, e.g. by super-enhancers and broad H3K4me3 domains. However, it remains unclear whether post-transcriptional regulation is also unique. We performed a systematic analysis of transcriptome-wide RNA stability in nine cell types found that unstable transcripts were enriched identity-related pathways while stable housekeeping pathways. Joint analyses chromatin state revealed...
Breast cancer stem-like cells (BCSCs) have been suggested as the underlying cause of tumor recurrence, metastasis and drug resistance in triple-negative breast (TNBC). Here, we report discovery biological evaluation a highly potent small-molecule antagonist exportin-1, LFS-1107. We ascertained that exportin-1 (also named CRM1) is main cellular target LFS-1107 by nuclear export functional assay, bio-layer interferometry binding assay C528S mutant cell line. found significantly inhibited TNBC...
Immunotherapy targeting tumor-associated macrophages (TAMs) is a promising approach to treating cancer. However, the limited drug targets and ambiguous mechanisms impede development of clinical immunotherapy strategies. To elucidate underlying processes involved in mononuclear phagocyte (MNP) infiltration phenotypic changes hepatocellular carcinoma (HCC), we integrated single-cell RNA-sequencing data from 100,030 cells derived patients with HCC healthy individuals compared phenotypes origins...
Progressive reduction in β-cell mass and function comprise the core of pathogenesis mechanism type 2 diabetes. The process deteriorating pancreatic islets, which a complex network molecular events is involved, not yet fully characterized. We used RNA sequencing tandem tag–based quantitative proteomics technology to measure temporal mRNA protein expression changes islets Goto-Kakizaki (GK) rats from 4 24 weeks age. Our omics data set outlines dynamics during deterioration GK as two stages:...
Long non-coding RNAs (lncRNAs) are receiving increasing attention as cancer markers for diagnosis and treatment. Although there many computational methods to identify lncRNAs, they do not comprehensively integrate multi-omics features predictions or systematically evaluate the contribution of each class omics multifaced landscape lncRNAs. In this study, we developed an algorithm, POCALI, lncRNAs by integrating 44 across six categories. We explored contributions different identifying and,...
Kinesin family member 13B (KIF13B), a crucial motor protein, exerts multiple cellular biological functions. However, the implication of KIF13B in metabolic dysfunction-associated fatty liver disease (MAFLD) has not been explored yet. This study aimed to investigate KIF13B's role and underlying mechanism MAFLD proposes it as potential pharmacological target. We assessed expression patients rodent models. The roles Kif13b lipid metabolism were investigated using whole-body knockout mice,...
Since PGAP (pan-genome analysis pipeline) was published in 2012, it has been widely employed bacterial genomics research. Though integrated several modules for pan-genomics analysis, how to properly and effectively interpret visualize the results data is still a challenge. To well present genomic characteristics, novel cross-platform software developed, named PGAP-X. Four kinds of were developed integrated: whole genome sequences alignment, orthologous genes clustering, pan-genome profile...
Several mutations in leucine rich repeat kinase 2 (LRRK2) gene have been associated with pathogenesis of Parkinson's disease (PD), a neurodegenerative disorder marked by resting tremors, and rigidity, leading to Postural instability. It has revealed that lead an increase activity LRRK2 protein are significantly PD pathogenesis. Recent studies shown some Rab GTPases, especially Rab8, serve as substrates undergo phosphorylation its switch II domain upon interaction. Current study was performed...
Abstract Conversion between cell types, e.g., by induced expression of master transcription factors, holds great promise for cellular therapy. Our ability to manipulate identity is constrained incomplete information on genes (CIGs) and their regulation. Here, we develop CEFCIG, an artificial intelligent framework uncover CIGs further define regulators. On the basis machine learning, CEFCIG reveals unique histone codes transcriptional regulation reported CIGs, utilizes these predict...
: Ferroptosis-driven loss of dopaminergic neurons plays a pivotal role in the pathogenesis Parkinson's disease (PD). In PD patients, Hspb1 is commonly observed at abnormally high levels substantia nigra. The precise consequences overexpression PD, however, have yet to be fully elucidated.
Pazopanib, a tyrosine kinase inhibitor marketed for cancer treatment, abrogates the course of neurodegeneration.