A5100587245- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Cardiomyopathy and Myosin Studies
- Carbon and Quantum Dots Applications
- Arsenic contamination and mitigation
- Endometrial and Cervical Cancer Treatments
- Zeolite Catalysis and Synthesis
- Nanocluster Synthesis and Applications
- Angiogenesis and VEGF in Cancer
- Cell death mechanisms and regulation
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Nanoplatforms for cancer theranostics
- Cancer survivorship and care
- Proteoglycans and glycosaminoglycans research
- DNA and Nucleic Acid Chemistry
- Color perception and design
- Color Science and Applications
- Vascular Tumors and Angiosarcomas
- DNA Repair Mechanisms
Huashan Hospital
2017-2024
Fudan University
2017-2024
Minzu University of China
2024
Gansu University of Traditional Chinese Medicine
2024
Qingdao University
2024
Gansu Agricultural University
2024
Tianjin University
2024
China Medical University
2024
Hexi University
2024
XinHua Hospital
2024
Targeting PLK1 has recently been proven as a viable therapeutic strategy against oesophageal squamous cell carcinom (ESCC). Therefore, this study aimed to explore whether the inhibitor BI2536 is able sensitize ESCC cells cisplatin (DDP) and determine underlying mechanisms.Viability, clonogenicity, cycle distribution apoptosis were assessed in treated with or DDP alone combination. Checkpoint activation was examined by immunoblotting immunohistochemistry. Xenograft model used assess efficacy...
Abstract Reprogrammed cellular metabolism is essential for maintaining cancer stem cells (CSCs) state. Here, we report that mitochondrial D-lactate catabolism a necessary initiating oncogenic event during tumorigenesis of esophageal squamous cell carcinoma (ESCC). We discover cyclin-dependent kinase 7 (CDK7) phosphorylates nuclear Yes-associated protein 1 (YAP) at S127 and S397 sites enhances its transcription function, which promotes dehydrogenase (LDHD) expression. Moreover, LDHD enriched...
Abstract Cachexia, characterized by loss of skeletal muscle mass and function, is estimated to inflict the majority patients with oesophageal squamous cell carcinoma (ESCC) associated their poor prognosis. However, its underlying mechanisms remain elusive. Here, we developed an ESCC‐induced cachexia mouse model using human xenograft ESCC lines found that ESCC‐derived extracellular vesicles (EVs) containing prolyl 4‐hydroxylase subunit beta (P4HB) induced apoptosis cells. We further...
Pancreatic cancer (PaCa) is one of the most intractable and fatal malignancies associated with dysregulation long noncoding RNAs (lncRNAs), which are a large class larger than 200 nt that act as competing endogenous or sponges for miRNAs to induce tumour biological behaviours. However, their clinical value in treating pancreatic has been poorly explained, but they essential improving prognosis PaCa patients.
Objectives The present study aims to elucidate the underlying mechanism how PFKP is regulated by BRCA1 and clinical significance of in breast cancer. Methods MEF-BRCA1△/△ wild type counterpart MEF-BRCA1+/+ cell lines were used test sensitivity glucose depletion culture medium. Glucose Assay Kit was quantify levels cultural supernatant lysate. Real time PCR measure mRNA expression genes. Western blot detect protein levels. Chromatin immunoprecipitation verify bindings between transcription...
Solute carrier family 38 (SLC38s) transporters play important roles in amino acid transportation and signaling transduction. However, their genetic alterations biological tumors are still largely unclear. This study aimed to elucidate the signatures of SLC38s implications esophageal squamous cell carcinoma (ESCC).Analyses on somatic mutation copy number (CNAs) SLC38A3 were performed as described. Immunohistochemistry (IHC) assay Western blot used detect protein expression level. MTS assay,...
An electrochemical immunoassay for cardiac troponin I (cTnI) combining the concepts of dual monoclonal antibody "sandwich" principle, silver enhancement on nano-gold particle, and anodic stripping voltammetry is described. Four main steps were carried out to obtain analytical signal, i.e., electrode preparation, immunoreaction, enhancement, voltammetric detection. A linear relationship between peak current concentration cTnl from 1 20 ng/ml a limit detection 0.8 obtained. The established...
The MDCK cell line is perceived as better than the embryos of hen eggs for production influenza vaccines, but tumorigenicity these cells concerning. Epidermal growth factor receptor (EGFR) likely to be a crucial target that contributes cells. In this study, EGFR-knockdown and EGFR-overexpression lines were established. EGFR’s influence on growth, migration, clonogenic ability, flu virus susceptibility was evaluated in vitro, its role examined nude mice. GST pull-down coupled with mass...
Two novel fluorophore (BODIPY)-bearing complexes, pyriplatin (mCBP) and pyrimidine-chelated cisplatin (dCBP), were synthesized characterized. The additional BODIPY-pyridine/pyridimine motifs of the two Pt(II) complexes resulted in stronger interactions with DNA comparison those cisplatin. mCBP caused relative decreases life span body length a resistant vivo model, N2 (wild-type) Caenorhabditis elegans. In contrast, dCBP dramatic reduction physiological parameters C. elegans, indicating high...
<title>Abstract</title> Purpose Cervical intraepithelial neoplasia (CIN) with co-existent vaginal (VaIN) is a challenge in clinical management. This study was aimed to evaluate the association of having CIN and VaIN persistent high-risk HPV (HR-HPV) infection residual/recurrent disease after treatment. Methods A retrospective performed involving women treated for CIN2/3 those only between January 2018 December 2020. LEEP, electrocoagulation (fulguration) at time First follow-up conducted by...
Abstract Colour in a Chinese painting not only conveys cultural, historical, and philosophical concepts but also carries specific symbolic meanings emotions, possessing high level of artistic value. However, the sensitivity paintings to light results exhibition environments being characterised by low illuminance colour temperature. Therefore, accurately assessing preferences under such conditions is an issue that needs be addressed. This study conducted simulated experiments real museum...
Objective/Background: Esophageal squamous cell carcinoma (ESCC) urgently requires more effective chemotherapies because of its relatively low overall survival rate. Targeting PLK1 has recently been proven as a viable therapeutic strategy against ESCC. Therefore, this study aimed to explore whether the inhibitor BI2536 is able sensitize ESCC cells cisplatin (DDP) and determine underlying mechanisms. Methods: Viability, clonogenicity, cycle distribution apoptosis were assessed in KYSE150...
The transcriptional repressor Pokemon (encoded by the Zbtb7 gene) is a critical factor in oncogenesis. overexpression leads to overt oncogenic transformation both vitro and vivo transgenic mice. objective of this study was investigate effect retrovirus expressing siRNA targeting human cervical cancer cells. We constructed identified recombinant particle gene, then testified suppression plasmid evaluated gene-silencing effect. got positive evaluation from colony forming experiment we found...