A5023495134- RNA modifications and cancer
- Esophageal Cancer Research and Treatment
- Cancer-related gene regulation
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Atherosclerosis and Cardiovascular Diseases
- Epigenetics and DNA Methylation
- SARS-CoV-2 detection and testing
- Electrowetting and Microfluidic Technologies
- Chemokine receptors and signaling
- SARS-CoV-2 and COVID-19 Research
- Circular RNAs in diseases
- Analytical Chemistry and Sensors
- Identification and Quantification in Food
- Cell Adhesion Molecules Research
- Bacteriophages and microbial interactions
- Cancer, Lipids, and Metabolism
- Advanced Biosensing Techniques and Applications
- RNA and protein synthesis mechanisms
- Axon Guidance and Neuronal Signaling
- CRISPR and Genetic Engineering
- Thermal Radiation and Cooling Technologies
- Apelin-related biomedical research
- Galectins and Cancer Biology
Shanghai Jiao Tong University
2015-2025
Jiangnan University
2025
Soochow University
2015-2023
Peking University
2020-2023
Peking University Cancer Hospital
2020-2023
University of Alberta
2017-2021
Alberta Hospital Edmonton
2021
University of Science and Technology of China
2021
Faculty (United Kingdom)
2021
Leiden University Medical Center
2017
Molecular diagnosis of COVID-19 primarily relies on the detection RNA SARS-CoV-2 virus, causative infectious agent pandemic. Reverse transcription polymerase chain reaction (RT-PCR) enables sensitive specific sequences genes that encode dependent (RdRP), nucleocapsid (N), envelope (E), and spike (S) proteins virus. Although RT-PCR tests have been widely used many alternative assays developed, current testing capacity availability cannot meet unprecedented global demands for rapid, reliable,...
We have developed a single-tube assay for SARS-CoV-2 in patient samples. This combined advantages of reverse transcription (RT) loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) enzyme Cas12a. Our is able to detect single tube within 40 min, requiring only temperature control (62 °C). The RT-LAMP reagents were added sample vial, while CRISPR Cas12a deposited onto lid vial. After half-hour...
The <italic>trans</italic>-cleavage activity of the target-activated CRISPR/Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly gold nanoparticles.
Accumulating evidence suggests a role of semaphorins in vascular homeostasis. Here, we investigate the Sema7A (semaphorin 7A) atherosclerosis and its underlying mechanism.Using genetically engineered Sema7A-/-ApoE-/- mice, showed that deletion attenuates atherosclerotic plaque formation primarily aorta ApoE-/- mice on high-fat diet. A higher level atheroprone lesser curvature correlation with disturbed flow. This notion is supported by elevated expression human umbilical venous endothelial...
Abstract Aims Chemoattractants and their cognate receptors are essential for leucocyte recruitment during atherogenesis, atherosclerotic plaques preferentially occur at predilection sites of the arterial wall with disturbed flow (d-flow). In profiling endothelial expression atypical chemoattractant (ACKRs), we found that Ackr5 (CCRL2) was up-regulated in an subpopulation by stimulation. We therefore investigated role CCRL2 its ligand chemerin atherosclerosis underlying mechanism. Methods...
Abstract Cachexia, characterized by loss of skeletal muscle mass and function, is estimated to inflict the majority patients with oesophageal squamous cell carcinoma (ESCC) associated their poor prognosis. However, its underlying mechanisms remain elusive. Here, we developed an ESCC‐induced cachexia mouse model using human xenograft ESCC lines found that ESCC‐derived extracellular vesicles (EVs) containing prolyl 4‐hydroxylase subunit beta (P4HB) induced apoptosis cells. We further...
RNA-cleaving DNAzymes and their multicomponent nucleic acid enzymes (MNAzymes) have been successfully used to detect acids proteins. The appropriate split of the catalytic cores is critical formation MNAzymes with high activities. However, for protein detection, no systematic investigation has made on effects locations secondary structures activities cleavage reaction. We systematically studied how affect activity that catalyze multiple steps. engineered basis DNAzyme 10-23 as a model...
Monoclonal antibodies (mAbs) are widely used in tumor and autoimmune disease therapy clinical diagnosis, but they suffer from self-aggregation, particularly dimer formation, impacting their stability, efficacy, potentially causing severe allergies. Traditional methods for detecting mAb dimers, such as TEM, AUC, DLS, SEC, CE, limited by low throughput, high costs, qualitative data, making them unsuitable large-scale sample assays biopharmaceuticals industry well antibody research. To address...
Passive daytime radiative cooling (PDRC) stands out as an eco-friendly, energy-independent solution, which has emerged a field of intense interest and extensive investigation. In this work, low-thickness PDRC coating with average solar reflectance 93.7% long-wave infrared emittance 98.5% was developed by combining the backscattering hollow silica microspheres (HSMs) surface scattering TiO2. A finite element method simulation conducted to verify that HSMs enhance performances. The synergistic...
Work has rarely been reported on a highly portable smartphone-based capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C4D). Herein, phone-based CE (130 mm × 190 70 mm, 1.4 kg) C4D and Bluetooth communication, as well user-interface software, was developed for analysis. To demonstrate the device, six metal ions were selected model analytes verification successfully applied to of in tap water. The analytical performance highlighted that runs data...
Breast cancer (BC) has become the leading cause of global incidence. Despite therapeutic advances, a critical unmet need persists for identifying novel targets. Our integrated bioinformatics analysis identified DTL, component Cullin-RING ligase (CRL) E3 ubiquitin family, as significantly upregulated in BC tissues. This upregulation correlated with poor patient prognosis, stemness, and metabolic reprogramming, which was driven by genetic alterations such gene amplification reduced promoter...
Esophageal squamous cell carcinoma (ESCC) is one of the most common and deadly diseases. In our previous comprehensive genomics study, we found that family with sequence similarity 135 member B (FAM135B) was a novel cancer-related gene, yet its biological functions molecular mechanisms remain unclear. this demonstrate protein levels FAM135B are significantly higher in ESCC tissues than precancerous tissues, high expression correlates poorer clinical prognosis. Ectopic promoted proliferation...
Obesity and related diseases are becoming a growing risk for public health around the world due to westernized lifestyle. Sema7A, an axonal guidance molecule, has been known play role in neurite growth, bone formation, immune regulation. Whether Sema7A participates obesity metabolic is unknown. As several SNPs SEMA7A its receptors were found correlate with BMI parameters human population, we investigated potential of hepatic steatosis.GWAS GEPIA database was used analyze correlation...
BACKGROUND. Targeted arterial infusion of verapamil combined with chemotherapy (TVCC) is an effective clinical interventional therapy for esophageal squamous cell carcinoma (ESCC), but multidrug resistance (MDR) remains the major cause relapse or poor prognosis, and underlying molecular mechanisms MDR, temporal intratumoral heterogeneity, clonal evolutionary processes have not been determined.