A5054236676- Antibiotic Resistance in Bacteria
- Advanced biosensing and bioanalysis techniques
- Bacterial biofilms and quorum sensing
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Catalytic Processes in Materials Science
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- SARS-CoV-2 detection and testing
- Ion channel regulation and function
- RNA Interference and Gene Delivery
- Catalysis and Oxidation Reactions
- Enzyme Production and Characterization
- Bone Metabolism and Diseases
- COVID-19 Clinical Research Studies
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Polymer Surface Interaction Studies
- Zeolite Catalysis and Synthesis
- Cardiac electrophysiology and arrhythmias
- Long-Term Effects of COVID-19
- Enterobacteriaceae and Cronobacter Research
- Cardiomyopathy and Myosin Studies
- Antimicrobial agents and applications
Jiangnan University
2025
University of Alberta
2020-2024
Henan University of Science and Technology
2017-2024
Grinm Advanced Materials (China)
2021-2024
State Key Laboratory of Advanced Materials for Smart Sensing
2021-2024
Institute of Plasma Physics
2024
Army Medical University
2012-2023
University of California, San Diego
2017-2023
Southwest Hospital
2012-2023
First Hospital of Jilin University
2021-2022
Molecular diagnosis of COVID-19 primarily relies on the detection RNA SARS-CoV-2 virus, causative infectious agent pandemic. Reverse transcription polymerase chain reaction (RT-PCR) enables sensitive specific sequences genes that encode dependent (RdRP), nucleocapsid (N), envelope (E), and spike (S) proteins virus. Although RT-PCR tests have been widely used many alternative assays developed, current testing capacity availability cannot meet unprecedented global demands for rapid, reliable,...
The study aimed to perform a systematic investigation of the effects quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa.The Ps. aeruginosa strain PAO1 was selected as test strain. results indicated that did not impact growth determined by MIC curve analysis. However, this compound significantly inhibited (P < 0·05) production including pyocyanin, protease elastase at lower concentration than those for most previously reported plant extracts substances. Considering...
We have developed a single-tube assay for SARS-CoV-2 in patient samples. This combined advantages of reverse transcription (RT) loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) enzyme Cas12a. Our is able to detect single tube within 40 min, requiring only temperature control (62 °C). The RT-LAMP reagents were added sample vial, while CRISPR Cas12a deposited onto lid vial. After half-hour...
Abstract The infusion of coronavirus disease 2019 (COVID-19) patients with mesenchymal stem cells (MSCs) potentially improves clinical symptoms, but the underlying mechanism remains unclear. We conducted a randomized, single-blind, placebo-controlled (29 patients/group) phase II trial to validate previous findings and explore potential mechanisms. Patients treated umbilical cord-derived MSCs exhibited shorter hospital stay ( P = 0.0198) less time required for symptoms remission 0.0194) than...
CRISPR-Cas systems integrated with nucleic acid amplification techniques improve both analytical specificity and sensitivity. We describe here issues solutions for the successful integration of reverse transcription (RT), recombinase polymerase (RPA), CRISPR-Cas12a nuclease reactions into a single tube under an isothermal condition (40 °C). Specific detection few copies viral DNA sequence was achieved in less than 20 min. However, sensitivity orders magnitude lower RNA due to slow initiation...
An injectable hydrogel wound dressing based on an oxidized dextran and ε-poly-<sc>l</sc>-lysine network has multiple biological activities.
Despite the excellent antimicrobial activity, high toxicity and low selectivity of cationic peptides (AMPs) their synthetic analogues impede biomedical applications. In this study, we report a series peptidopolysaccharides synthesized by thiol-ene click chemistry grafting polypeptides, methacrylate-ended poly(lysine- random-phenylalanine) (Me-K nF m), onto thiolated polysaccharide (dextran, Dex) backbone. Their copolymers (Dex- g-K m) exhibit potent broad-spectrum antibacterial antifungal...
The position of the nucleus in a cell is controlled by interactions between linker nucleoskeleton and cytoskeleton (LINC) complex cytoskeleton. Defects nuclear positioning abnormal aggregation nuclei occur many muscle diseases correlate with dysfunction. Nesprin 1, which includes multiple isoforms, an integral component LINC complex, critical for anchorage skeletal muscle, thought to provide essential link actin. However, previous studies have yet identify isoform responsible. To elucidate...
Abstract A total of 26 bla IMP-4 -carrying strains Pseudomonas aeruginosa and Klebsiella pneumoniae were isolated from 2009 to 2013 in a Chinese teaching hospital, these can be assigned into multiple sequence types or allelic profiles as determined by multilocus typing. Of strains, P. P378 K. 1220 harbor the IMP-4-encoding plasmids pP378-IMP p1220-IMP, respectively, whose complete nucleotide sequences are genetically closely related IncN1-type plasmid pIMP-HZ1. pP378-IMP/p1220-IMP-like...
Abstract Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is a powerful tool to manipulate the genome with extraordinary simplicity and speed. To generate genetically modified animals, CRISPR/Cas9-mediated editing typically accomplished by microinjection of mixture Cas9 DNA/mRNA single-guide RNA (sgRNA) into zygotes. However, sgRNAs used for this approach require manipulation via molecular cloning as well in vitro...
Abstract In this work, a novel biomimetic surface‐attachable initiator is successfully synthesized by the conjugation of 3,4‐dihydroxyphenylacetic acid and thermal 2,2′‐azobis(2‐methylpropionamide) dihydrochloride (V‐50). The (DOPV) can adhere to various material surfaces in mussel‐inspired way initiate surface grafting polymerization. Hydrogel coatings are facilely prepared thermal‐initiated radical copolymerization antimicrobial polyhexamethylene guanidine antifouling polyethylene glycol...
Background: Cardiomyopathy is a major clinical feature in Barth syndrome (BTHS), an X-linked mitochondrial lipid disorder caused by mutations Tafazzin ( TAZ ), encoding acyltransferase required for cardiolipin remodeling. Despite recent description of mouse model BTHS cardiomyopathy, in-depth analysis specific abnormalities and form function vivo cardiomyopathy lacking. Methods: We performed assessment cardiac function, species profiles, structure our newly generated Taz...
This paper proposes to apply a newly developed Non-Dispersive Infrared Spectroscopy (NDIR) gas sensing system composed of pyroelectric infrared detectors monitor the thermal runaway (TR) process lithium-ion batteries in real time and achieve an early warning for battery TR process. The new Electrical Vehicle Safety—Global Technical Regulation (EVS-GTR) requires that be provided passengers at least five minutes before serious incident. experimental results indicate carbon dioxide methane were...