A5006989054- Cardiomyopathy and Myosin Studies
- Mitochondrial Function and Pathology
- Heat shock proteins research
- ATP Synthase and ATPases Research
- Nuclear Structure and Function
- Congenital heart defects research
- Cardiovascular Effects of Exercise
- Birth, Development, and Health
- RNA Research and Splicing
- DNA and Biological Computing
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Calcium signaling and nucleotide metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Renin-Angiotensin System Studies
- Genetic Neurodegenerative Diseases
- Signaling Pathways in Disease
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
- Acute Ischemic Stroke Management
- Nanopore and Nanochannel Transport Studies
Anhui Medical University
2023-2024
University of California, San Diego
2015-2024
Second Hospital of Anhui Medical University
2024
University of Georgia
2018-2024
Zhejiang University
2024
Shanghai Institute of Technology
2024
Peking University Shenzhen Hospital
2022
Yale University
2022
King's College London
2018
Peking University
2009-2016
Defective protein quality control (PQC) systems are implicated in multiple diseases. Molecular chaperones and co-chaperones play a central role functioning PQC. Constant mechanical metabolic stress cardiomyocytes places great demand on the PQC system. Mutation downregulation of co-chaperone BCL-2-associated athanogene 3 (BAG3) associated with cardiac myopathy heart failure, BAG3 E455K mutation leads to dilated cardiomyopathy (DCM). However, mechanisms by which DCM remain obscure. Here, we...
To maintain healthy mitochondrial enzyme content and function, mitochondria possess a complex protein quality control system, which is composed of different endogenous sets chaperones proteases. Heat shock 60 (HSP60) one these molecular has been proposed to play pivotal role in the regulation folding prevention aggregation. However, physiological function HSP60 mammalian tissues not fully understood. Here we generated an inducible cardiac-specific knockout mouse model, demonstrated that...
Adipose tissue is a key endocrine organ that governs systemic homeostasis. PPARγ master regulator of adipose signaling plays an essential role in insulin sensitivity, making it important therapeutic target. The selective agonist rosiglitazone (RSG) has been used to treat diabetes. However, adverse cardiovascular effects have seriously hindered its clinical application. Experimental models revealed activation increases cardiac hypertrophy. RSG stimulates hypertrophy and oxidative stress...
Inositol 1, 4, 5-trisphosphate receptor-mediated (IP3R-mediated) calcium (Ca2+) release has been proposed to play an important role in regulating vascular smooth muscle cell (VSMC) contraction for decades. However, whether and how IP3R regulates blood pressure vivo remains unclear. To address these questions, we have generated a muscle-specific triple-knockout (smTKO) mouse model using tamoxifen-inducible system. In this study, the of IP3R-mediated Ca2+ adult VSMCs on aortic contractility...
The position of the nucleus in a cell is controlled by interactions between linker nucleoskeleton and cytoskeleton (LINC) complex cytoskeleton. Defects nuclear positioning abnormal aggregation nuclei occur many muscle diseases correlate with dysfunction. Nesprin 1, which includes multiple isoforms, an integral component LINC complex, critical for anchorage skeletal muscle, thought to provide essential link actin. However, previous studies have yet identify isoform responsible. To elucidate...
Small heat shock protein HSPB7 is highly expressed in the heart. Several mutations within are associated with dilated cardiomyopathy and heart failure human patients. However, precise role of still unclear. In this study, we generated global as well cardiac-specific KO mouse models found that loss globally or specifically cardiomyocytes resulted embryonic lethality before day 12.5. Using biochemical cell culture assays, identified an actin filament length regulator repressed polymerization...
Membrane contact sites are fundamental for transmission and translation of signals in multicellular organisms. The junctional membrane complexes the cardiac dyads, where transverse (T) tubules juxtaposed to sarcoplasmic reticulum, a prime example. T-tubule uncoupling remodeling well-known features disease heart failure. Even subtle alterations association between T-tubules reticulum can cause serious disorders. NEXN (nexilin) has been identified as an actin-binding protein, multiple...
Stroke is a major cause of death and long-term disability affecting seven million adults in the United States each year. Recently, it has been demonstrated that neurological diseases, associated pathology, susceptibility changes correlated with gut microbiota. However, microbial community stroke not well characterized. The acute stage critical period for assessing injury severity, therapeutic intervention, clinical prognosis. We investigated microbiota composition diversity using middle...
Background: Cardiomyopathy is a major clinical feature in Barth syndrome (BTHS), an X-linked mitochondrial lipid disorder caused by mutations Tafazzin ( TAZ ), encoding acyltransferase required for cardiolipin remodeling. Despite recent description of mouse model BTHS cardiomyopathy, in-depth analysis specific abnormalities and form function vivo cardiomyopathy lacking. Methods: We performed assessment cardiac function, species profiles, structure our newly generated Taz...
Endothelial cells (ECs) are constantly exposed to xenobiotics and endobiotics or their metabolites, which perturb EC function, as well shear stress, plays a crucial role in vascular homeostasis. Pregnane X receptor (PXR) is nuclear key regulator of the detoxification xeno- endobiotics. Here we show that laminar stress (LSS), atheroprotective flow, activates PXR ECs, whereas oscillatory atheroprone suppresses PXR. LSS activation cultured ECs led increased expression target gene, multidrug...
An elevated plasma level of 5-hydroxytryptamine (5-HT) or upregulation 5-HT receptor signaling both is implicated in vascular contraction and remodeling pulmonary arterial hypertension (PAH). Recently, peroxisome proliferator-activated receptor-γ (PPARγ) agonists have been shown to ameliorate PAH. However, their effects on the 5-HT-induced arteries remain unknown. Here, we examined role PPARγ inhibiting 5-HT2B (5-HT2BR) Pulmonary from PAH rats induced by monocrotaline chronic hypoxia showed...
Arrhythmogenic cardiomyopathy (AC) is associated with mutations in genes encoding intercalated disc proteins and ultimately results sudden cardiac death. A subset of patients AC have the autosomal recessive cardiocutaneous disorder Naxos disease, which caused by a 2-base pair deletion plakoglobin-encoding gene JUP that truncated protein reduced expression. In mice, cardiomyocyte-specific plakoglobin deficiency recapitulates many aspects human AC, overexpression Naxos-associated also an...
Luma is a recently discovered, evolutionarily conserved protein expressed in mammalian heart, which associated with the LInker of Nucleoskeleton and Cytoskeleton (LINC) complex. The LINC complex structurally integrates nucleus cytoplasm plays critical role mechanotransduction across nuclear envelope. Mutations several components both humans mice result various cardiomyopathies, implying they play essential, non-redundant roles. A single amino acid substitution serine 358 to leucine (S358L)...
Abstract Intracellular calcium (Ca2+) mobilization after engagement of the BCR has been proposed to play an important role in B cell development and function. activation causes initial Ca2+ release from endoplasmic reticulum that is mediated by inositol 1,4,5-trisphosphate receptor (IP3R) then triggers store-operated entry once store depleted. Store-operated shown regulate function but dispensable for development. By contrast, IP3R-mediated cells remains be determined. In this study, we...
Nanopore-based sensors have been studied extensively as potential tools for DNA sequencing, characterization of epigenetic modifications such 5-methylcytosine, and detection microRNA biomarkers. In the studies described here, α-hemolysin protein nanopore embedded in a lipid bilayer was used interstrand cross-links duplex DNA. Interstrand are important lesions medicinal chemistry toxicology because they prevent strand separation that is required read-out genetic information from cells....
Background Endothelial NO synthase plays a central role in regulating vasodilation and blood pressure. Intracellular Ca