A5009570320- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Soybean genetics and cultivation
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Luminescence and Fluorescent Materials
- Crystallization and Solubility Studies
- Glycosylation and Glycoproteins Research
- X-ray Diffraction in Crystallography
- Virus-based gene therapy research
- Insect and Pesticide Research
- Neurobiology and Insect Physiology Research
- Porphyrin and Phthalocyanine Chemistry
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Molecular Sensors and Ion Detection
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
- Peroxisome Proliferator-Activated Receptors
- Supramolecular Chemistry and Complexes
- Eicosanoids and Hypertension Pharmacology
- Complement system in diseases
- Cytomegalovirus and herpesvirus research
- Enzyme Structure and Function
Wuhan University of Technology
2012-2024
Jilin Agricultural University
2024
State Grid Corporation of China (China)
2023-2024
Yangzhou University
2021-2023
Jinan University
2013-2022
China Three Gorges University
2020-2022
University of Electronic Science and Technology of China
2021
Tianjin Economic-Technological Development Area
2021
Huizhou Central People's Hospital
2020
Southwest Jiaotong University
2020
Overexpression of classically activated macrophages (M1) subtypes and assessed reactive oxygen species (ROS) levels are often observed in patients with ulcerative colitis. At present, the treatment system these two problems has yet to be established. Here, chemotherapy drug curcumin (CCM) is decorated Prussian blue analogs a straightforward cost-saving manner. Modified CCM can released inflammatory tissue (acidic environment), eventually causing M1 transform into M2 inhibiting...
Orphan nuclear receptors have been instrumental in identifying novel signaling pathways and therapeutic targets. However, identification of ligands for these has often based on random compound screens or other biased approaches. As a result, it remains unclear many cases if the reported are true endogenous ligands,--i.e., ligand that is bound to receptor an unperturbed vivo setting. Technical limitations limited our ability identify this rigorous definition. The orphan hepatocyte factor 4...
Abstract The molecular mechanics Poisson–Boltzmann surface area (MM‐PBSA) method for GROMACS (g_mmpbsa) is an open‐source tool that capable of reading the trajectories generated by and calculating binding free energy using MM‐PBSA method. However, there are multiple force fields available users to choose from in software, also different solvent water models combine with chosen fields. These combinations parameters may significantly impact results g_mmpbsa calculation. Unfortunately, exact...
A gelatin/sodium alginate-based hydrogel microsphere has been fabricated after reaction condition optimization. Macrophages (RAW246.7) and adipose mesenchymal stem cells (ADSC) have subsequently encapsulated in the order to construct a 3D paracrine system for wound healing treatment. The synthesized displayed neglectable cytotoxicity toward both until 10 days of incubation, indicating promising biocompatibility microsphere. qRT-PCR ELISA experiment revealed positive regulation cytokines...
Estrogen receptor-alpha (ERalpha) has been identified in the male reproductive tract, but role of estrogen not well characterized. In vivo mutations ERalpha genes have demonstrated necessity for ERalpha-mediated action fertility. We asked whether both ERbeta messenger RNA and protein were present tract wild-type knock-out (ERalpha KO) mice, could compensate lack infertile KO mice. Immunohistochemical localization with N- C-terminal anti-ERbeta antibodies that is Leydig cells testes...
Wheat stripe rust poses a marked threat to global wheat production. Accurate and effective disease severity assessments are crucial for resistance breeding timely management of field diseases. In this study, we propose practical solution using mobile-based deep learning model-assisted labeling. StripeRust-Pocket, user-friendly mobile application developed based on models, accurately quantifies in leaf images, even under complex backgrounds. Additionally, StripeRust-Pocket facilitates image...
Renewable energy serves as an effective alternative to traditional fossil fuels, reducing reliance on imports from specific countries, thereby alleviating geopolitical risks and ensuring national security. The development utilization of renewable have profound implications for the global consumption structure landscape. This paper utilizes time-series panel data 1990 2020 examine causal relationship between risks. Focusing primarily United States Kingdom, a multivariate regression model was...
The potential role of estrogen receptor-β (ERβ) in normal ovarian folliculogenesis and reproductive disorders such as follicular cysts has not been well defined. Therefore, we were interested cloning, sequencing, localizing ERβ mRNA protein within the bovine ovary. Bovine (bERβ) was amplified by reverse transcription-polymerase chain reaction (RT-PCR), then cloned sequenced. Results showed that open reading frame bERβ cDNA spanned 1584 nucleotides encoding a 527 amino acids. N-terminal...
Human immunodeficiency virus 1 (HIV-1) protease (PR) plays a crucial role in the maturation of virus. The study substrate specificity HIV-1 PR as new endeavor strives to increase our ability understand how recognizes its various cleavage sites. To predict sites, most existing approaches have been developed solely based on homogeneity sequence information with supervised classification techniques. Although efficient, these are found be restricted explaining their results and probably provide...
An understanding of the interaction between antibody and its targeted antigen knowing epitopes are critical for development monoclonal drugs. Complement factor H (CFH) is implied to play a role in tumor growth metastasis. autoantibody CHF associated with anti-tumor cell activity. The human Ab42 that was isolated from cancer patient CFH polypeptide (pCFH) analyzed by molecular docking, dynamics (MD) simulation, free energy calculation, computational alanine scanning (CAS). Experimental (EAS)...
Steady laminar flow is atheroprotective, in part because of its antiinflammatory effects on vascular endothelial cells (ECs). We studied the activation peroxisome proliferator-activated receptor-gamma (PPARgamma) ECs response to and associated effect.Using channel with cultured ECs, we found that activated PPARgamma-mediated PPAR-responsive element (PPRE) activity increased mRNA encoding CD36, a PPARgamma-targeted gene. Analysis CD36 promoter revealed PPRE was required for activation....
T cell functional exhaustion during chronic hepatitis B virus (HBV) infection may contribute to the failed viral clearance; however, underlying molecular mechanisms remain largely unknown. Here we demonstrate that jumonji domain-containing protein 6 (JMJD6) is a potential regulator of proliferation HBV infection. The expression JMJD6 was reduced in lymphocytes (CHB) patients and this reduction associated with impaired proliferation. Moreover, silencing primary human cells We found promotes...
Abstract The burgeoning functions of many microRNAs (miRs) have been well study in cancer. However, the level and function miR-1205 laryngeal squamous cell cancer remains unknown. In current research, we validated that was notably downregulated human carcinoma (LSCC) samples comparison with tissues adjacent to LSCC, correlated T stage, lymph node metastasis, clinical stage. Using Kaplan–Meier analysis indicates high expression has a favorable prognosis for patients LSCC. Functional assays...
1-17-2 is a rat anti-human DEC-205 monoclonal antibody that induces internalization and delivers antigen to dendritic cells (DCs). The potentially clinical application of this limited by its murine origin. Traditional humanization method such as complementarity determining regions (CDRs) graft often leads decreased or even lost affinity. Here we have developed novel based on computer modeling bioinformatics analysis. First, used homology technology build the precise model Fab. A epitope...
Athetis lepigone is a polyphagous pest found around the world that feeds on maize, wheat, and various other important crops. Although it exhibits degree of resistance to chemical insecticides, an effective pest-control method has not yet been developed. The sex pheromone communication system plays essential role in mating reproduction moths, which pheromone-binding proteins (PBPs) are crucial genes. In this study, we cloned purified protein AlepPBP1 using E. coli expression had higher...
Insect resistance to insecticides is an increasingly serious problem, and the resistant mechanisms are complicated. The research based on chemosensory pathway one of hot problems at present, but specific binding mechanism genes remains elusive. AlepGOBP2 (belong insect gene) with two was investigated by computational experimental approaches. Our calculation results indicated that four key residues (Phe12, Ile52, Ile94, Phe118) could steadily interact these be assigned as hotspot sites...
Human adenoviruses (HAdVs) are the etiologic agent of many human infectious diseases. The existence at least 54 different serotypes HAdVs has resulted in difficulties clinical diagnosis. Acute respiratory tract disease (ARD) caused by some from B and C species is particularly serious. Hexon, main coat protein HAdV, contains major serotype-specific cell epitopes; however, few studies have addressed epitope mapping most HAdV serotypes. In this study, we utilized a novel rapid method for...