A5066605214- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- interferon and immune responses
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- ATP Synthase and ATPases Research
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Genetics, Aging, and Longevity in Model Organisms
- Genetics and Neurodevelopmental Disorders
- RNA and protein synthesis mechanisms
- Apelin-related biomedical research
- Immune Cell Function and Interaction
- RNA regulation and disease
- Amyotrophic Lateral Sclerosis Research
- Trace Elements in Health
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolism and Genetic Disorders
- Paraoxonase enzyme and polymorphisms
- Adipose Tissue and Metabolism
- Neurogenetic and Muscular Disorders Research
- Cardiac Fibrosis and Remodeling
- T-cell and B-cell Immunology
Institute of Laboratory Animal Science
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
National Health and Family Planning Commission
2017-2024
Academy of Medical Sciences
2020-2021
Beijing Institute of Technology
2019
Significance Type I IFN signaling is maintained under homeostatic conditions, which plays a crucial role in antiviral immunity. Epigenetic regulation of innate immunity and inflammation attracts much attention now. However, the underlying mechanisms need further investigation. Here, we demonstrate that N 6 -methyladenosine (m A) “reader” YT521-B homology domain-containing family 3 (YTHDF3) selectively inhibits IFN-stimulated gene expression basal conditions by binding to translation...
Long noncoding RNAs (lncRNAs) involved in the regulation of antiviral innate immune responses need to be further identified. By functionally screening lncRNAs macrophages, here we identified lncRNA
Significance Upon virus infection, host cells detect viral nucleic acids and initiate antiviral innate immune responses by producing type I IFNs proinflammatory cytokines. As a cytoplasmic sensor for RNA viruses, retinoic acid-inducible gene 1 (RIG-I) recognizes the pathogenic RNAs activates downstream signaling initiation of immunity. In this study, we identify RING finger protein 122 (RNF122) as an E3 ligase that directly binds to RIG-I induce K48-linked ubiquitination mediate degradation,...
The CRISPR/Cas9 system has been proven to be an efficient gene-editing tool for genome modification of cells and organisms. Multiplex genetic engineering in rat holds a bright future the study complex disease. Here, we show that this enables simultaneous disruption four genes (ApoE, B2m, Prf1, Prkdc) rats one-step, by co-injection Cas9 mRNA sgRNAs into fertilized eggs. We further observed gene modifications are germline transmittable, confirmed off-target mutagenesis mosaicism rarely...
Abstract Pseudouridine is the most prevalent RNA modification, and its aberrant function implicated in various human diseases. However, specific impact of pseudouridylation on hematopoiesis remains poorly understood. Here, we investigated role transfer (tRNA) erythropoiesis association with mitochondrial myopathy, lactic acidosis, sideroblastic anemia syndrome (MLASA) pathogenesis. By using patient-specific induced pluripotent stem cells (iPSCs) carrying a genetic pseudouridine synthase 1...
Precise modifications such as site mutation, codon replacement, insertion or precise targeted deletion are needed for studies of accurate gene function. The CRISPR/Cas9 system has been proved a powerful tool to generate knockout and knockin animals. But the homologous recombination (HR)-directed genetic modification mediated by is relatively lower compared with nonhomologous end-joining (NHEJ) pathway extremely expected be improved. Here, in this study 2 strategies were used increase rats....
Cytochrome P450 2E1 (CYP2E1) is a cytochrome enzyme that catalyzes the metabolism of toxic substrates. CYP2E1 upregulated in heart disease, including dilated cardiomyopathy (DCM) mouse model. Here, knockdown significantly ameliorated left ventricle, thin wall, and dysfunctional contraction cTnT R141W adriamycin-induced DCM models. Interstitial fibrosis, poorly organized myofibrils, swollen mitochondria with loss cristae were improved myocardium α-myosin heavy chain (MHC)-cTnT ×CYP2E1-silence...
Recent studies have indicated that a number of long noncoding RNAs (lncRNAs) are dysregulated in hepatocellular carcinoma, while their aberrant expressions associated with tumorigenesis and poor prognosis. To identify hepatitis B virus (HBV)‐related lncRNAs, we used RNA deep sequencing to quantify the abundances lncRNAs HepG2 cells HBV transgenic HepG2‐4D14 cells. Here, demonstrate lnc‐HUR1 is significantly upregulated We found HBV‐encoded x protein can enhance transcription lnc‐HUR1....
Abstract Leptin receptor, which is encoded by the diabetes ( db ) gene and highly expressed in choroid plexus, regulatesenergy homeostasis, balance between food intake energy expenditure, fertility bone mass. Here, using CRISPR/Cas9 technology, we created leptin receptor knockout rat. Homozygous null rats are characterized obesity, hyperphagia, hyperglycemia, glucose intolerance, hyperinsulinemia dyslipidemia. Due to long-term poor glycemic control, also develop some diabetic complications...
Significance Epithelial cell–mediated chemokine production and subsequent neutrophil recruitment are important for pathogen clearance, which, however, also closely related to severe inflammatory tissue damage during lung infection, especially influenza virus infection the current pandemic coronavirus infection. Certain regulation underlying mechanisms of expression in epithelial cells remain largely unknown. Here, by identifying mouse long noncoding RNA lnc-Cxcl2 human lnc-CXCL2-4-1...
Pseudouridylation plays a regulatory role in various physiological and pathological processes. A prime example is the mitochondrial myopathy, lactic acidosis, sideroblastic anemia syndrome (MLASA), characterized by defective pseudouridylation resulting from genetic mutations pseudouridine synthase 1 (PUS1). However, roles mechanisms of normal erythropoiesis MLASA-related remain elusive. We established mouse model carrying point mutation (R110W) enzymatic domain PUS1, mimicking common human MLASA.