A5013934880- Advanced Electron Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- Electron and X-Ray Spectroscopy Techniques
- RNA modifications and cancer
- Force Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Hepatitis C virus research
- Methane Hydrates and Related Phenomena
- Indigenous Studies and Ecology
- Geological Studies and Exploration
- Climate change and permafrost
- Geology and Paleoclimatology Research
- Natural product bioactivities and synthesis
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Phylogenetic Studies
- Monoclonal and Polyclonal Antibodies Research
- Cryospheric studies and observations
- Phytochemistry and Biological Activities
- Pharmacological Effects and Assays
- Viral Infections and Outbreaks Research
- Integrated Circuits and Semiconductor Failure Analysis
- Enzyme Structure and Function
- Phytochemistry and Bioactivity Studies
- Spectroscopy and Quantum Chemical Studies
University of Chinese Academy of Sciences
2019-2025
Institute of Biophysics
2014-2025
Chinese Academy of Sciences
2008-2025
Shanghai Institute of Materia Medica
2023-2025
Czech Academy of Sciences, Institute of Biophysics
2020
Institute of Oceanology
2008-2011
Abstract The rapid generation of various species and strains laboratory animals using CRISPR/Cas9 technology has dramatically accelerated the interrogation gene function in vivo . So far, dominant approach for genotyping genome-modified been T7E1 endonuclease cleavage assay. Here, we present a polyacrylamide gel electrophoresis-based (PAGE) method to genotype mice harboring different types indel mutations. We developed 6 system utilized this from F0 F2 generation, which included single...
Abstract Cryo-electron tomography is a major tool used to study the structure of protein complexes in situ. However, throughput tilt-series image data collection still quite low. Here, we show that GisSPA, GPU accelerated program, can translationally and rotationally localize target complex cellular lamellae, as prepared with focused ion beam, using single cryo-electron microscopy images without tilt-series, reconstruct at near-atomic resolution. GisSPA allows high-throughput acquisition...
RBM39 is an essential component of the spliceosome, playing a critical role in maintaining mRNA integrity. Its depletion significantly exacerbates RNA splicing defects and demonstrates potent anticancer activity. To identify key effectors following depletion, we employed multiomics approach to directly compare two structurally distinct compounds, CB039 Indisulam. Through proteomic analysis, sequencing, DepMap dependency assessment, CEP192 emerged as crucial gene, exhibiting 96% 1,100...
The aim of this study is to test the effect environmental protection tax on financial performance A-share listed companies in Shanghai and Shenzhen from 2016 2021. To achieve this, a differences-in-differences model was established explore impact tax, agency costs, enterprise performance. analysis specifically examines how implementation influences outcomes function costs between them. results indicate that introduction an notably lowers increases Chinese companies. Furthermore, serve as...
•Structures could be achieved when proteins are overlapped with surroundings free of tilt series•The particle detection efficiency is significantly improved•Allowing the usage homolog as templates•The throughput structure determination remarkably enhanced Cryo-electron tomography a powerful tool for in native environment. However, this method requires acquisition series, which time-consuming and severely slows determination. By treating densities non-target protein non-Gaussian noise, we...
Abstract Many protein complexes are highly dynamic in cells, characterizing their conformational changes cells is crucial for unraveling functions. In this report, using cryo-electron microscopy, 451,700 ribosome particles from Saccharomyces cerevisiae cell lamellae were obtained to solve the 60S region 2.9 Å resolution by situ single particle analysis. Over 20 distinct conformations identified 3D classification with resolutions typically higher than 4 Å. These used reconstruct a complete...
Sclareolide is a sesquiterpene lactone isolated from various plant sources in tons every year and commercially used as flavor ingredient the cosmetic food industries. Antitumor antiviral activities of sclareolide have been previously reported. However, biological studies synthetic analogous are few. In view these, we developed robust method that allows assembly 36 novel sclareolide-indole conjugates their derivatives. The was based on TiCl4-promoted nucleophilic substitution...
The frequency-dependent signal to noise ratio of cryo-electron microscopy data varies dramatically with the frequency and type data. During different steps processing, distinct SNR are used for calculations. Thus, specific weighting function based on particular should be designed optimize corresponding calculation. Here, we deduced these functions by maximizing cross correlated coefficients. Some our refinement resemble that in existing software packages. However, motion correction, particle...
Abstract Cryo-electron microscopy (cryo-EM) tomography is a powerful tool for in situ structure determination. However, this method requires the acquisition of tilt series, and its time consuming throughput acquiring series severely slows determination structures. By treating electron densities non-target protein as non-Gaussian distributed noise, we developed new target function that greatly improves efficiency recognition single cryo-EM image without series. Moreover, sorting effectively...
Abstract Cryo-electron microscopy (cryo-EM) tomography is a powerful tool for in situ structure determination. However, this method requires the acquisition of tilt series, and its time consuming throughput acquiring series severely slows determination structures. By treating electron densities non-target protein as non-Gaussian distributed noise, we developed new target function that greatly improves efficiency recognition single cryo-EM image without series. Moreover, sorting effectively...