A5024725065- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Alzheimer's disease research and treatments
- Cancer-related gene regulation
- Nanoplatforms for cancer theranostics
- Metal-Catalyzed Oxygenation Mechanisms
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Bone Metabolism and Diseases
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Esophageal Cancer Research and Treatment
- RNA and protein synthesis mechanisms
- Ferroptosis and cancer prognosis
- Gastric Cancer Management and Outcomes
- Enzyme Structure and Function
- S100 Proteins and Annexins
- Genetics and Neurodevelopmental Disorders
- Esophageal and GI Pathology
- Advanced biosensing and bioanalysis techniques
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Beijing University of Agriculture
2021-2024
Soochow University
2016-2024
University of Macau
2017-2024
Second Affiliated Hospital of Soochow University
2016-2024
The Fourth Affiliated Hospital of Soochow University (Suzhou Dushu Lake Hospital)
2024
Xuzhou Medical College
2015-2023
First Affiliated Hospital of Anhui Medical University
2012-2023
Anhui Medical University
2011-2023
Air Force Medical University
2006-2023
Abstract While the oxygen-dependent reversal of lysine N ɛ -methylation is well established, existence bona fide ω -methylarginine demethylases (RDMs) controversial. Lysine demethylation, as catalysed by two families (the flavin-dependent KDM1 enzymes and 2-oxoglutarate- JmjC KDMs, respectively), proceeds via oxidation -methyl group, resulting in release formaldehyde. Here we report detailed biochemical studies clearly demonstrating that, purified form, a subset KDMs can also act RDMs, both...
Abstract Suffering from the laborious synthesis and undesirable tumor microenvironment response, exploitation of novel NIR‐II absorbing organic photothermal agents is importance to promote phototherapeutic efficacy. Herein, two kinds charge‐transfer complex nanoparticles (TMB‐F4TCNQ TMB‐TCNQ) are prepared by supramolecular assembly. Because larger energy gap between donor acceptor, TMB‐F4TCNQ presents higher degree (72 %) than that TMB‐TCNQ (48 in nanoaggregates. Therefore, exhibits stronger...
Abstract The emergence of multidrug resistant bacteria has resulted in plenty stubborn nosocomial infections and severely threatens human health. Developing novel bactericide therapeutic strategy is urgently needed. Herein, mesoporous silica supported silver–bismuth nanoparticles (Ag‐Bi@SiO 2 NPs) are constructed for synergistic antibacterial therapy. In vitro experiments indicate that the hyperthermia originating from Bi NPs can disrupt cell integrity accelerate Ag ions release, further...
Mutations of human PHF8 cluster within its JmjC encoding exons and are linked to mental retardation (MR) a cleft lip/palate phenotype. Sequence comparisons, employing structural insights, suggest that contains the double stranded beta-helix fold ferrous iron binding residues present in 2-oxoglutarate-dependent oxygenases. We report recombinant is an Fe(II) N(epsilon)-methyl lysine demethylase, which acts on histone substrates. selective vitro for N(epsilon)-di- mono-methylated does not...
Abstract The response to hypoxia in animals involves the expression of multiple genes regulated by αβ-hypoxia-inducible transcription factors (HIFs). hypoxia-sensing mechanism oxygen limited hydroxylation prolyl residues N- and C-terminal oxygen-dependent degradation domains (NODD CODD) HIFα isoforms, as catalysed hydroxylases (PHD 1–3). Prolyl promotes binding von Hippel–Lindau protein (VHL)–elongin B/C complex, thus signalling for proteosomal HIFα. We reveal that certain PHD2 variants...
Significance The processing of DNA sequences into proteins is fine-tuned to meet the conflicting demands accuracy and speed. mutations can introduce premature stop codons, leading inactive proteins. We report that oxygen-dependent posttranslational modification ribosomal decoding center affects codon readthrough in an mRNA sequence-dependent manner. Our work demonstrates oxygenases catalyzing RPS23 hydroxylation are conserved eukaryotes, including yeasts, flies, humans. In basal undergoes...
Abstract Tumor hypoxia strengthens tumor resistance to different therapies especially oxygen involved strategies, such as photodynamic therapy (PDT). Herein, the thermal responsive phase change materials (PCM) are utilized coencapsulate ultrasmall manganese dioxide (sMnO 2 ) and organic photosensitizer IR780 obtain IR780‐sMnO ‐PCM nanoparticles for controlled modulation enhanced phototherapy. The protective PCM layer can not only prevent from photodegradation, but also immediately release...
Tumor cells of colorectal cancer (CRC) release exosomes into the circulation. These can mediate communication between and affect various tumor‐related processes in their target cells. We present a quantitative proteomics analysis purified from serum patients with CRC normal volunteers; data are available via ProteomeXchange identifier PXD003875. identified 918 proteins an overlap 725 Gene IDs Exocarta list. Compared serum‐purified (SPEs) volunteers, we found 36 upregulated 22 downregulated...
Significance Members of the 2-oxoglutarate (2OG)-dependent oxygenase superfamily catalyze a range important biological oxidations. Structurally informed bioinformatic predictions suggest that human genome encodes as yet unassigned members superfamily. We describe work demonstrating 2OG and Fe(II)-dependent domain-containing protein 1 (OGFOD1) is hydroxylase modifies small ribosomal subunit RPS23 at conserved prolyl residue in ribosome-decoding center suppression or deletion OGFOD1 associated...
Efficient stop codon recognition and peptidyl-tRNA hydrolysis are essential in order to terminate translational elongation maintain protein sequence fidelity. Eukaryotic termination is mediated by a release factor complex that includes eukaryotic 1 (eRF1) eRF3. The N terminus of eRF1 contains highly conserved motifs couple at the ribosomal A site hydrolysis. We reveal Jumonji domain-containing 4 (Jmjd4), 2-oxoglutarate- Fe(II)-dependent oxygenase, catalyzes carbon (C4) lysyl hydroxylation...
Abstract Introduction We investigated the proteomic profiles of amyloid plaques (APs) from Alzheimer's disease (AD) and age‐matched non‐AD brains APP/PS1 transgenic model mice. Methods APs adjacent control regions were collected fresh‐frozen brain sections using laser capture dissection. Proteins quantitated tag‐labeling coupled high‐throughput mass spectra. Results Over 4000 proteins accurately quantified, more than 40 identified as highly enriched in both AD APs, including apoE, midkine,...
Signal transduction from toll-like receptors (TLRs) is important for innate immunity against infections, but deregulated TLR signalling contributes to inflammatory disorders. Here we show that myeloid cell-specific ablation of TRAF2 greatly promotes TLR-stimulated proinflammatory cytokine expression in macrophages and exacerbates colitis an animal model bowel disease. deficiency does not enhance upstream events, it causes accumulation two transcription factors, c-Rel IRF5, known mediate...
microRNA (miRNA) dysregulation is involved in the development and progression of various human cancers, including hepatocellular carcinoma (HCC). However, how to identify miRNAs targeting specific mRNA cells a significant challenge because interaction complexity limited knowledge rules governing these processes. Some are not predictable by current computer algorithms available. Here, using p21 as target, we established new method, called miRNA vivo precipitation (miRIP), which kind can...