A5088499046- Psychotherapy Techniques and Applications
- RNA modifications and cancer
- Academic and Historical Perspectives in Psychology
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Mental Health and Psychiatry
- Plant biochemistry and biosynthesis
- Marine Invertebrate Physiology and Ecology
- NF-κB Signaling Pathways
- Personality Disorders and Psychopathology
- Transactional Analysis in Psychotherapy
- RNA and protein synthesis mechanisms
- Chemical synthesis and alkaloids
- Microbial Natural Products and Biosynthesis
- Metal-Catalyzed Oxygenation Mechanisms
- Mitochondrial Function and Pathology
- Pancreatic and Hepatic Oncology Research
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Allelopathy and phytotoxic interactions
- Autophagy in Disease and Therapy
- Biochemical and biochemical processes
- Nicotinic Acetylcholine Receptors Study
- Cellular transport and secretion
Qiagen (Germany)
2016-2023
Lead Discovery Center (Germany)
2011-2023
Helmholtz Zentrum München
2013-2020
University of Arkansas at Fayetteville
2019
University of Oxford
2011-2014
Justus-Liebig-Universität Gießen
2011
Radboud University Nijmegen
2011
Radboud University Medical Center
2011
University Medical Center Hamburg-Eppendorf
2011
Universität Hamburg
2011
Hydra, first described by Anton van Leeuwenhoek in a letter to the Royal Society 1702, has been studied biologists for centuries and now is an important model work on axial patterning, stem cell biology regeneration. Its genome, over half of which made up mobile elements, sequenced, as genome Curvibacter sp. bacterium stably associated with Hydra magnipapillata. Comparisons those other animals provide insights into evolution epithelia, contractile tissues, developmentally regulated...
Modifying the Modifier Covalent modification of proteins provides an important means whereby their function is regulated. Hydroxylation, catalyzed by oxygenase enzymes, plays role in response to hypoxia, for example. The human protein Jmjd6 has been thought act as oxygenase, catalyzing demethylation histone H3 at arginine-2 and H4 arginine-3. Webby et al. (p. 90 ) now show that interacts with messenger RNA splicing factor U2AF65 acts hydroxylate this lysine residues, modifications also seen...
Significance The processing of DNA sequences into proteins is fine-tuned to meet the conflicting demands accuracy and speed. mutations can introduce premature stop codons, leading inactive proteins. We report that oxygen-dependent posttranslational modification ribosomal decoding center affects codon readthrough in an mRNA sequence-dependent manner. Our work demonstrates oxygenases catalyzing RPS23 hydroxylation are conserved eukaryotes, including yeasts, flies, humans. In basal undergoes...
Significance Members of the 2-oxoglutarate (2OG)-dependent oxygenase superfamily catalyze a range important biological oxidations. Structurally informed bioinformatic predictions suggest that human genome encodes as yet unassigned members superfamily. We describe work demonstrating 2OG and Fe(II)-dependent domain-containing protein 1 (OGFOD1) is hydroxylase modifies small ribosomal subunit RPS23 at conserved prolyl residue in ribosome-decoding center suppression or deletion OGFOD1 associated...
ABSTRACT Protein kinases represent central and multifunctional regulators of a balanced virus-host interaction. Cyclin-dependent protein kinase 7 (CDK7) plays crucial regulatory roles in cell cycle transcription, both connected with the replication many viruses. Previously, we developed CDK7 inhibitor, LDC4297, that inhibits vitro nano-picomolar range. Novel data from kinome-wide evaluation (>330 profiled ) demonstrate selectivity. Importantly, provide first evidence for antiviral...
De novo mutations in ATAD3A (ATPase family AAA-domain containing protein 3A) were recently found to cause a neurological syndrome with developmental delay, hypotonia, spasticity, optic atrophy, axonal neuropathy, and hypertrophic cardiomyopathy. Using whole-exome sequencing, we identified dominantly inherited heterozygous variant c.1064G > A (p.G355D) mother presenting hereditary spastic paraplegia (HSP) neuropathy her son dyskinetic cerebral palsy, both disease onset childhood. HSP is...
Background Methylation of residues in histone tails is part a network that regulates gene expression. JmjC domain containing proteins catalyze the oxidative removal methyl groups on lysine residues. Here, we report studies to test involvement Jumonji domain-containing protein 6 (Jmjd6) demethylation. Jmjd6 has recently been shown hydroxylate RNA splicing factors and known be essential for differentiation multiple tissues cells during embryogenesis. However, there have conflicting reports as...
The Fe(II) and 2-oxoglutarate dependent oxygenase Jmjd6 has been shown to hydroxylate lysine residues in the essential splice factor U2 auxiliary 65 kDa subunit (U2AF65) act as a modulator of alternative splicing. We describe further evidence for role regulation pre-mRNA processing including interactions with multiple arginine–serine-rich (RS)-domains SR- SR-related proteins U2AF65, Luc7-like protein 3 (Luc7L3), SRSF11 Acinus S′, but not bona fide RS-domain SRSF1. identified target are...
Abstract Nerve cells and spontaneous coordinated behavior first appeared near the base of animal evolution in common ancestor cnidarians bilaterians. Experiments on cnidarian Hydra have demonstrated that nerve are essential for this behavior, although organized a diffuse network do not form ganglia. Here we show gap junction protein innexin-2 is expressed small group lower body column an anti-innexin-2 antibody binds to junctions same region. Treatment live animals with eliminates staining...
Jmjd6 (jumonji-domain-containing protein 6) is an Fe(II)- and 2OG (2-oxoglutarate)-dependent oxygenase that catalyses hydroxylation of lysine residues in proteins involved pre-mRNA splicing. plays essential role vertebrate embryonic development has been shown to modulate alternative splicing response hypoxic stress. In the present study we show alternatively spliced version lacking polyS (polyserine) domain localizes nucleolus, predominantly fibrillar centre. with deleted also interacts...
ABSTRACT The Fe(II) and 2-oxoglutarate-dependent oxygenase Alkb homologue 1 (Alkbh1) has been shown to act on a wide range of substrates, like DNA, tRNA histones. Thereby different enzymatic activities have identified including, among others, demethylation N3-methylcytosine (m3C) in RNA- single-stranded DNA oligonucleotides, N1-methyladenosine (m1A) or formation 5-formyl cytosine (f5C) tRNA. In accordance with the Alkbh1 also proposed reside distinct cellular compartments human mouse cells,...
Colony-stimulating factor-1 receptor (CSF1R) is a tyrosine kinase that controls the differentiation and maintenance of most tissue-resident macrophages, inhibition CSF1R has been suggested as possible therapy for range human disorders. Herein, we present synthesis, development, structure–activity relationship series highly selective pyrrolo[2,3-d]pyrimidines, showing subnanomolar enzymatic this with excellent selectivity toward other kinases in platelet-derived growth factor (PDGFR) family....
Objective— Inhibitor of apoptosis proteins (IAPs), such as X-linked or cellular IAP 1/2 (XIAP, cIAP1/2), are important regulators apoptosis. antagonists currently under clinical investigation anticancer agents. Interestingly, IAPs participate in the inflammation-associated TNF receptor signaling complex and regulate NFκB signaling. This raises question about role inflammation. Here, we investigated anti-inflammatory potential inhibitors inflammatory processes endothelial cells. Methods...