A5030678508- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Immune Cell Function and Interaction
- Protein Degradation and Inhibitors
- Cytomegalovirus and herpesvirus research
- Epigenetics and DNA Methylation
- Herpesvirus Infections and Treatments
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Viral-associated cancers and disorders
- T-cell and B-cell Immunology
- Attention Deficit Hyperactivity Disorder
- Animal Virus Infections Studies
- Cellular transport and secretion
- RNA modifications and cancer
- Prostate Cancer Treatment and Research
- DNA and Nucleic Acid Chemistry
- Calcium signaling and nucleotide metabolism
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Cell death mechanisms and regulation
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
Korea University
2009-2025
University of California, San Diego
2013-2024
Sejong University
2024
Samsung (South Korea)
2021-2024
Sungkyunkwan University
2024
Howard Hughes Medical Institute
2013-2021
Samsung Changwon Hospital
2021
Changwon National University
2021
UC San Diego Health System
2020
University of Southern California
2009-2015
Abstract Autophagy-related factors are implicated in metabolic adaptation and cancer metastasis. However, the role of autophagy progression their effect treatment response remain largely elusive. Recent studies have shown that UVRAG, a key autophagic tumour suppressor, is mutated common human cancers. Here we demonstrate cancer-related UVRAG frameshift (FS), which does not result null mutation, expressed as truncated FS colorectal (CRC) with microsatellite instability (MSI), promotes...
γ-herpesviruses (γHVs) have developed an interaction with their hosts wherein they establish a life-long persistent infection and are associated the onset of various malignancies. One critical virulence factor involved in persistency murine γ-herpesvirus 68 (γHV68) is viral homolog Bcl-2 protein (vBcl-2), which has been implicated to counteract both host apoptotic responses autophagy pathway. However, relative significance two activities vBcl-2 yet be elucidated. Here, by characterizing...
Significance The emergence of powerful new chemical library-screening approaches and the generation types structures makes novel methods available to link candidate chemicals potential target genes, e.g., as in interaction with effects on chromatin-bound targets. Here we report a method that can provide genome-wide location drug. One such synthetic chemical, SD70—first identified screen for inhibitors tumor translocation events—was resynthesized tag permitting ChIP-sequencing–like analysis,...
Cisplatin is an antineoplastic drug administered at suboptimal and intermittent doses to avoid life-threatening effects. Although this regimen shortly improves symptoms in the short term, it also leads more malignant disease long term. We describe a multilayered analysis ranging from chromatin translation-integrating immunoprecipitation sequencing (ChIP-seq), global run-on (GRO-seq), RNA (RNA-seq), ribosome profiling-to understand how cisplatin confers (pre)malignant features by using...
The eukaryotic genome is partitioned into distinct topological domains separated by boundary elements. Emerging data support the concept that several well-established nuclear compartments are ribonucleoprotein condensates assembled through physical process of phase separation. Here, based on our demonstration chemical disruption condensate assembly weakens insulation properties a specific subset (∼20%) topologically associated domain (TAD) boundaries, we report disrupted boundaries...
Abstract Metabolic dysfunction-associated steatohepatitis (MASH) is associated with the activation of Kupffer cells (KCs) and hepatic stellate cells, at which point a metabolically stressed hepatocyte becomes integral to progression disease. We observed significant reduction in level alpha-1-antitrypsin (A1AT), hepatocyte-derived secreted factor, both patients MASH mice fed fast-food diet (FFD). KC-mediated inflammation, most notably IL-1β, led transcriptional inhibition A1AT by HNF4α. In...
Significance Negative-strand RNA viruses are highly pathogenic and cause many severe diseases in humans animals. These generally use existing cellular pathways to enter cells, which involves intensive interaction with the endomembrane network, offering endocytic pathway as an attractive scheme for therapeutic intervention. Molecular mechanisms governing virus entry remain incompletely understood. We found that UV-radiation resistance-associated gene, well known regulating autophagy...
Introduction Diagnosis of Attention Deficit/Hyperactivity Disorder (ADHD) is based on clinical evaluation symptoms by a psychiatrist, referencing results psychological tests. When diagnosing ADHD, the child’s behavior and functionality in real-life situations are critical components. However, direct observation clinician often not feasible practice. Therefore, such information typically gathered from primary caregivers or teachers, which can introduce subjective elements. To overcome these...
Background Despite its initial positive response to hormone ablation therapy, prostate cancers invariably recur in more aggressive, treatment resistant forms. The lack of our understanding underlying genetic alterations for the transition from androgen-dependent (AD) ADI cancer growth hampers ability develop target-driven therapeutic strategies efficient cancer. Methodology/Principal Findings By screening a library activated human kinases, we have identified TPL2, encoding serine/threonine...
Significance Enhancers are crucial regulatory elements that dictate cellular processes by spatiotemporally controlling promoter activity. Recent studies have shown active enhancers themselves generate noncoding enhancer RNAs (eRNAs) play a critical role in fine tuning gene expression. Using genomic approaches, we demonstrate oncogenic KSHV modulates host activity PELs to control its lifecycle. During latency, expression levels of several key transcription factors and oncogenes elevated...
ABSTRACT Despite all the advantages that cell‐cultured influenza vaccines have over egg‐based vaccines, inferior productivity of cell‐culture systems is a major drawback must be addressed. BST‐2 (tetherin) host restriction factor which inhibits budding‐out various enveloped viruses from infected cells. We developed BST‐2‐deficient MDCK and Vero cell lines to increase virus release in culture. gene knock‐out resulted increased viral particles into culture medium, by at least 2‐fold up 50‐fold...