A5004607331- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- RNA Research and Splicing
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Pharmacological Receptor Mechanisms and Effects
- RNA regulation and disease
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Circular RNAs in diseases
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Mass Spectrometry Techniques and Applications
- Immune Cell Function and Interaction
- RNA modifications and cancer
University of North Carolina at Chapel Hill
2019-2025
Abstract We present the nuclear magnetic resonance spectroscopy (NMR) solution structure of 5′-terminal stem loop 5_SL1 (SL1) SARS-CoV-2 genome. SL1 contains two A-form helical elements and regions with non-canonical structure, namely an apical pyrimidine-rich asymmetric internal one nucleotides at 5′- 3′-terminal part sequence, respectively. The conformational ensemble representing averaged was validated using NMR residual dipolar coupling (RDC) small-angle X-ray scattering (SAXS) data....
RNA molecules undergo conformational transitions in response to cellular and environmental stimuli. Site-specific protonation, a fundamental chemical property, can alter the landscape of regulate their functions. However, characterizing protonation-coupled ensembles on large scale remains challenging. Here, we present pH-differential mutational profiling (PD-MaP) with dimethyl sulfate probing for high-throughput detection RNA. We demonstrated this approach microRNA-21 precursor (pre-miR-21)...
An array of carbohydrates masks the HIV-1 surface protein Env, contributing to evasion humoral immunity. In most isolates 'glycan holes' occur due natural sequence variation, potentially revealing underlying immune system. Here we computationally design epitopes that mimic such features (carbohydrate-occluded neutralization or CONE) Env through 'epitope transplantation', in which target region is presented on a carrier scaffold with preserved structural properties. Scaffolds displaying four...
Residual dipolar couplings (RDCs) are increasingly used for high-throughput NMR-based structural studies and to provide long-range angular constraints validate refine structures of various molecules determined by X-ray crystallography NMR spectroscopy. RDCs a given molecule can be measured in an anisotropic environment that aligns external magnetic field. Here, we demonstrate the first application polymer-based nanodiscs measurement from nucleic acids. Polymer-based prepared using negatively...
Abstract Background MeCP2 and MBD2 are members of a family proteins that possess domain selectively binds 5-methylcytosine in CpG context. Members the interact with other to modulate DNA packing. Stretching DNA–protein complexes nanofluidic channels cross-section few persistence lengths allows us probe degree compaction by proteins. Results We demonstrate while does not affect configuration. By using atomic force microscopy (AFM), we determined mechanism for is formation bridges between...
Abstract The G protein‐coupled μ‐opioid receptor (μ‐OR) mediates the majority of analgesia effects for morphine and other pain relievers. Despite extensive studies its structure activation mechanisms, inherently low maturation efficiency μ‐OR represents a major hurdle to understanding function. Here we computationally designed mutants with altered stability probe relationship between cell‐surface targeting, signal transduction, agonist efficacy. stabilizing mutation T315Y enhanced...
Abstract Our group recently discovered that the circular RNA (circRNA), CDR1as, promotes lung cancer metastasis in part through stabilization of coding gene, CDR1. The purpose this study was to identify intramolecular secondary structures CDR1as and determine whether there is a functional relationship between structure non-small cell (NSCLC) metastasis. We employed chemical probing approach selective 2’- hydroxyl acylation analyzed by primer extension mutational profiling (SHAPE-MaP)...