A5036192386- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Virology and Viral Diseases
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Studies on Chitinases and Chitosanases
- RNA regulation and disease
- HIV Research and Treatment
- Malaria Research and Control
University of North Carolina at Chapel Hill
2017-2025
It is unclear why the ability of Zika virus (ZIKV) to cause serious disease, including Guillain-Barré syndrome and birth defects, was not recognized until recent outbreaks. One contributing factor could be genetic differences between contemporary ZIKV strains historical strains. All isolates from outbreaks encode a viral envelope protein that glycosylated, whereas many lack this glycosylation. We generated nonglycosylated mutants evaluated their virulence in mice. found viruses were...
ABSTRACT Zika virus (ZIKV), a mosquito-borne flavivirus discovered in 1947, has only recently caused large outbreaks and emerged as significant human pathogen. In 2015, ZIKV was detected Brazil, the resulting epidemic spread throughout Western Hemisphere. Severe complications from infection include neurological disorders such Guillain-Barré syndrome adults variety of fetal abnormalities, including microcephaly, blindness, placental insufficiency, demise. There is an urgent need for tools...
We currently have an incomplete understanding of why only a fraction human antibodies that bind to flaviviruses block infection cells. Here we define the footprint strongly neutralizing monoclonal antibody (mAb G9E) with Zika virus (ZIKV) by both X-ray crystallography and cryo-electron microscopy. Flavivirus envelope (E) glycoproteins are present as homodimers on virion surface, G9E bound quaternary structure epitope spanning E protomers forming homodimer. As mainly neutralized ZIKV blocking...
Recent Zika virus (ZIKV) outbreaks and unexpected clinical manifestations of ZIKV infection have prompted an increase in ZIKV-related research. Here, we identify two strain-specific determinants virulence mice. We found that strain H/PF/2013 caused 100% lethality Ifnar1-/- mice, whereas PRVABC59 no lethality; both strains Ifnar1-/-Ifngr1-/- double-knockout (DKO) Deep sequencing revealed a high-frequency variant not present H/PF/2013: G-to-T change at nucleotide 1965 producing Val-to-Leu...
Abstract Biomolecules continually sample alternative conformations. Consequently, even the most energetically favored ground conformational state has a finite lifetime. Here, we show that, in addition to 3D structure, lifetime of determines its biological activity. Using hydrogen-deuterium exchange nuclear magnetic resonance spectroscopy, found that Zika virus exoribonuclease-resistant RNA (xrRNA) encodes with is ~10 5 –10 7 longer than canonical base pairs. Mutations shorten apparent...
Abstract We currently have an incomplete understanding of why only a fraction human antibodies that bind to flaviviruses block infection cells. Here we define the footprint strongly neutralizing monoclonal antibody (mAb G9E) with Zika virus (ZIKV) by both X-ray crystallography and cryo-electron microscopy. Flavivirus envelope (E) glycoproteins are present as homodimers on virion surface, G9E bound quaternary structure epitope spanning E protomers forming homodimer. As mainly neutralized ZIKV...