A5100372071- Extracellular vesicles in disease
- Microtubule and mitosis dynamics
- MicroRNA in disease regulation
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Liver Disease Diagnosis and Treatment
- Genetic and Kidney Cyst Diseases
- Ubiquitin and proteasome pathways
- Product Development and Customization
- Influenza Virus Research Studies
- Quality Function Deployment in Product Design
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Reproductive Biology and Fertility
- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- Cancer-related molecular mechanisms research
- Nonlinear Waves and Solitons
- Cervical Cancer and HPV Research
- Color perception and design
- Enzyme Catalysis and Immobilization
- Respiratory viral infections research
- Nonlinear Photonic Systems
- Epigenetics and DNA Methylation
Education University of Hong Kong
2024-2025
Second Hospital of Tianjin Medical University
2025
First Affiliated Hospital Zhejiang University
2024
Yale University
2008-2024
Baylor Genetics
2022-2024
Chinese University of Hong Kong
2024
Henan University of Science and Technology
2024
Nanjing Agricultural University
2021-2024
China Jiliang University
2024
Whitney Museum of American Art
2024
The histone demethylase PHF8 has been implicated in multiple pathological disorders, including X-linked mental retardation and tumorigenesis. However, it is not clear how the abundance function of are regulated. Here, we report that physically associates with deubiquitinase USP7. Specifically, demonstrated USP7 promotes deubiquitination stabilization PHF8, leading to upregulation a group genes, cyclin A2, critical for cell growth proliferation. USP7-encoding gene was also transcriptionally...
Diaryl ketones are important building blocks for synthesizing pharmaceuticals and generally regarded as "difficult-to-reduce" due to the large steric hindrance of their two bulky aromatic side chains. Alcohol dehydrogenase from Kluyveromyces polyspora (KpADH) has been identified a robust biocatalyst its high conversion diaryl ketone substrate (4-chlorophenyl)(pyridine-2-yl)ketone (CPMK) with moderate R-selectivity 82% ee. To modulate stereoselectivity KpADH, "polarity scanning" strategy was...
Abstract Thousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Coordination among multiple OADs is essential for generating mechanical forces bend microtubule doublets (MTDs). Using electron microscopy, we determined high-resolution structures Tetrahymena thermophila OAD arrays bound MTDs two different states. preferentially binds MTD protofilaments with pattern resembling native tracks its distinct microtubule-binding domains. Upon binding, free...
Abstract Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival development of nearly all eukaryotic species. An essential regulator conserved central apparatus (CA) motile cilia, composed a pair microtubules (C1 C2) associated with hundreds protein subunits per repeating unit. It largely unclear how CA plays its regulatory roles motility. Here, we present high-resolution structures Chlamydomonas reinhardtii by...
A hydrogel system loaded with mesenchymal stem cell-derived exosome (MSC-Exos) is an attractive new tool for tissue regeneration. However, the effect of stiffness exosome-loaded hydrogels on regeneration unclear. Here, role stiffness, during injured nerves, was assessed in vivo. The results showed that photocrosslinkable hyaluronic acid methacrylate plays important repairing nerve injury. Compared stiff exosomes, soft exosomes better repair peripheral nerves. promoted by quickly releasing to...
Leukemia stem cells (LSCs) play the major role in relapse of acute myeloid leukemia (AML). Recent evidence indicates that microvesicles (MVs) released from cancer can promote tumor growth and invasion. In this study, we investigated whether LSCs-released MVs (LMVs) regulate malignance AML overexpression suppressive microRNA (miR), miR34a, is able to interrupt process. LSCs were transfected with miRNA control (miRCtrl) or miR34a mimic for producing LMVs, respectively, defined as LMVs(miRCtrl)...
A hydroclassified combinatorial saturation mutagenesis (HCSM) strategy was proposed for reshaping the substrate binding pocket by dividing 20 amino acids into four groups based on their hydrophobicity and size. These smart HCSM libraries could significantly reduce screening effort especially simultaneous of three or more residues lacking high throughput methods. Employing strategy, stereoselectivity KpADH, an alcohol dehydrogenase from Kluyveromyces polysporus, efficiently improved to 99.4%...
Whether transcriptional regulators are functionally involved in mitosis is a fundamental question cell biology. Here we report that the RNF20/40 complex, major ubiquitin ligase catalysing histone H2B monoubiquitination, interacts with motor protein Eg5 during and participates spindle assembly. We show complex monoubiquitinates stabilizes Eg5. Loss of results assembly defects, cycle arrest apoptosis. Consistently, depletion either or suppresses breast cancer vivo. Significantly, concurrently...
Establishing a successful pregnancy depends on the endometrium and embryo. It is estimated that suboptimal endometrial receptivity account for one-third of implantation failures. Despite indepth understanding processes associated with embryo-endometrial cross-talk, little progress has been achieved diagnosis treatments receptivity.This retrospective study included women undergoing their first frozen-thawed embryo transfer (FET) cycles at our reproductive medicine center from March 2021 to...
Abstract Ossification of the posterior longitudinal ligament (OPLL) is an emerging spinal disease caused by heterotopic ossification ligament. The pathological mechanism poorly understood, which hinders development nonsurgical treatments. Here, we set out to explore function and small extracellular vesicles (sEVs) in OPLL. Global miRNA sequencings are performed on sEVs derived from cells normal OPLL patients, have showed that miR-320e abundantly expressed OPLL-derived compare other sEVs....
The binary Darboux transformation method is applied to the coupled Sasa-Satsuma equations, which can be used describe propagation dynamics of femtosecond vector solitons in birefringent fibers with third-order dispersion, self-steepening, and stimulated Raman scattering higher-order effects. An N-fold iterative formula resulting presented terms quasideterminants. Via simplest case this formula, a few illustrative explicit solutions equations are generated from vanishing non-vanishing...
Although accumulating evidence indicates that exosomes have a positive therapeutic effect on hepatic ischemia-reperfusion injury (HIRI), studies focusing the alleviation of liver by derived from adipose-derived mesenchymal stem cells (ADSCs-Exo) based inhibition cell pyroptosis not yet been reported. Exosomes contain different kinds biologically active substances such as proteins, lipids, mRNAs, miRNAs, and signaling molecules. These molecules are widely involved in cell-cell communication,...
Bone marrow mesenchymal stem cells (BMMSCs) exert protective effects against myocardial infarction (MI). Here, we focused on the function and mechanism of miR-455-3p from BMMSCs-derived exosomes (BMMSCs-Exo) in infarction.BMMSCs were isolated rat bone marrow, culture medium BMMSCs separated, administered to H9C2 under hypoxia-reperfusion (H/R) stimulation. MTT TUNEL staining analyzed cell viability apoptosis, respectively. RT-qPCR determined expression. Apoptosis-related proteins,...
Abstract A real‐time PCR assay was developed to identify varicella‐zoster virus (VZV) and herpes simplex (HSV) DNA in clinical specimens from subjects with suspected zoster (HZ; shingles). Three sets of primers probes were used separate reactions detect discriminate among wild‐type VZV (VZV‐WT), Oka vaccine strain (VZV‐Oka), HSV DNA, the reaction for each multiplexed probe specific human β‐globin gene assess specimen adequacy. Discrimination all VZV‐WT strains, including Japanese isolates...