A5087096006- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical effects in animals
- Aldose Reductase and Taurine
- Mitochondrial Function and Pathology
- Hearing, Cochlea, Tinnitus, Genetics
- Neurogenesis and neuroplasticity mechanisms
- Global Public Health Policies and Epidemiology
- Amino Acid Enzymes and Metabolism
- Biochemical Analysis and Sensing Techniques
- Electrolyte and hormonal disorders
- Alzheimer's disease research and treatments
- Neuroscience of respiration and sleep
- Epilepsy research and treatment
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Memory and Neural Mechanisms
- Photoreceptor and optogenetics research
- Prenatal Substance Exposure Effects
- Connexins and lens biology
- Cellular transport and secretion
- Neuropeptides and Animal Physiology
- Cerebrospinal fluid and hydrocephalus
University of Oslo
2016-2025
Karolinska Institutet
1995-2025
Charité - Universitätsmedizin Berlin
2024-2025
World Health Organization Regional Office for Europe
2023
Centre Virchow-Villermé
2023
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2004-2021
657 Oslo
2020
Oslo University Hospital
2009-2013
Johns Hopkins University
2001-2005
Yale University
2003-2005
Membrane water transport is critically involved in brain volume homeostasis and the pathogenesis of edema. The cDNA encoding aquaporin-4 (AQP4) channel protein was recently isolated from rat brain. We used immunocytochemistry high-resolution immunogold electron microscopy to identify cells membrane domains that mediate flux through AQP4. AQP4 abundant glial bordering subarachnoidal space, ventricles, blood vessels. also osmosensory areas, including supraoptic nucleus subfornical organ....
Abstract Antisera were raised against γ‐aminobutyric acid (GABA) or glutamate (Glu) conjugated to bovine serum albumin with glutaraldehyde. After purification, these antisera reacted strongly fixed GABA Glu, but not significantly other amino acids glutaraldehyde brain macromolecules. The used demonstrate the distributions of Glu‐like and GABA‐like immunoreactivities (Glu‐LI GABA‐LI) in parts perfusion‐fixed mouse rat brain, including olfactory bulb, cerebral neocortex, thalamus, basal...
Abstract The unravelling of the polarized distribution AQP4 in perivascular astrocytic endfeet has revitalized interest role astrocytes controlling water and ion exchange at brain–blood interface. importance is based on premise that they constitute a complete coverage vessel wall. Despite number studies different microscopic techniques this question yet to be resolved. We have made an electron 3D reconstruction CA1 (stratum moleculare) rat hippocampus. interdigitate overlap, leaving no slits...
The PARK8 gene responsible for late-onset autosomal dominant Parkinson's disease encodes a large novel protein of unknown biological function termed leucine-rich repeat kinase 2 (LRRK2). studies herein explore the localization LRRK2 in mammalian brain.Polyclonal antibodies generated against amino or carboxy termini were used to examine biochemical, subcellular, and immunohistochemical distribution LRRK2.LRRK2 is detected rat brain as an approximate 280kDa by Western blot analysis....
The water channel AQP4 is concentrated in perivascular and subpial membrane domains of brain astrocytes. These membranes form the interface between neuropil extracerebral liquid spaces. anchored at these by its carboxyl terminus to α-syntrophin, an adapter protein associated with dystrophin. To test functions pool, we studied mice homozygous for targeted disruption gene encoding α-syntrophin (α- Syn −/− ). animals show a marked loss from but no decrease other domains, as judged quantitative...
Abstract The corticoamygdaloid and intraamygdaloid projections of the rat were studied by use retrograde transport horseradish peroxidase (HRP). Observations based on anterograde enzyme exploited to determine course intrinsic connections. HRP was injected stereotactically means iontophoresis. Most amygdaloid nuclei selectively injected, all but a few reached more than one approach. vast majority fibers found originate in cortical areas defined as allocortical (Stephan, 1975). From medial...
The Aquaporin-4 (AQP4) water channel contributes to brain homeostasis in perivascular astrocyte endfeet where it is concentrated. We postulated that AQP4 tethered at this site by binding of the C terminus P SD95- D iscs large- Z O1 (PDZ) domain syntrophin, a component dystrophin protein complex. Chemical cross-linking and coimmunoprecipitations from demonstrated association with complex, including dystrophin, β-dystroglycan, syntrophin. expression was studied skeletal muscle mice lacking...
Abstract Two vesicular glutamate transporters, VGLUT1 and VGLUT2, have recently been identified, it has reported that they are expressed by largely nonoverlapping populations of glutamatergic neurons in the brain. We used immunocytochemistry with antibodies against both together markers for various spinal neurons, an attempt to identify interneurons dorsal horn mid‐lumbar cord rat. The great majority (94–100%) nonprimary axonal boutons contained somatostatin, substance P or neurotensin, as...
The water permeability of cell membranes differs by orders magnitude, and most this variability reflects the differential expression aquaporin channels. We have recently found that CNS contains a member family, aquaporin-4 (AQP4). As prerequisite for understanding cellular handling during neuronal activity, we investigated subcellular AQP4 in retina optic nerve where activity-dependent ion fluxes been studied detail. In situ hybridization with digoxigenin-labeled riboprobes immunogold...
Both homozygous (L166P, M26I, deletion) and heterozygous mutations (D149A, A104T) in the DJ-1 gene have been identified Parkinson's disease (PD) patients. The biochemical function subcellular localization of protein not clarified. To date has largely described studies over-expressing tagged vitro. It is known whether over-expressed identical to that endogenously expressed both vitro vivo. clarify DJ-1, we generated three highly specific antibodies investigated endogenous mouse brain tissues...
Sensitive and high-resolution immunocytochemical procedures were used to investigate the spatial organization of AMPA receptor subunits (GluR1–4) at synapse between inner hair cells afferent dendrites in rat organ Corti. This is a with special functional properties presynaptic dense body that defines center facilitates its morphometric analysis. A quantitative postembedding analysis was performed on specimens had been embedded metachrylate resin low temperature after freeze substitution....
Abstract The glymphatic system, that is aquaporin 4 (AQP4) facilitated exchange of CSF with interstitial fluid (ISF), may provide a clearance pathway for protein species such as amyloid-β and tau, which accumulate in the brain Alzheimer’s disease. Further, tau transference via extracellular space, compartment cleared by pathway, allows its neuron-to-neuron propagation, regional progression tauopathy disorder. system therefore represents an exciting new target Here we aim to understand...
Regulatory volume decrease (RVD) is a key mechanism for control that serves to prevent detrimental swelling in response hypo-osmotic stress. The molecular basis of RVD not understood. Here we show complex containing aquaporin-4 (AQP4) and transient receptor potential vanilloid 4 (TRPV4) essential astrocytes. Astrocytes from AQP4-KO mice astrocytes treated with TRPV4 siRNA fail respond hypotonic stress by increased intracellular Ca 2+ RVD. Coimmunoprecipitation immunohistochemistry analyses...
By the use of retrograde transport horseradish peroxidase (HRP), projections from thalamus to amygdala were investigated in rat and cat, with main emphasis on former species. HRP was injected stereotactically by microiontophoresis various amygdaloid nuclei. Several control procedures including different approaches for introduction micropipette undertaken eliminate possibility misinterpretation due uptake protein adjacent structures or fibers en passant. The paraventricular paratenial nuclei...