A5101652217- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cancer-related gene regulation
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Head and Neck Cancer Studies
- Genetics and Neurodevelopmental Disorders
- Lung Cancer Treatments and Mutations
- interferon and immune responses
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Fibroblast Growth Factor Research
- Nuclear Structure and Function
- Single-cell and spatial transcriptomics
- Drug Transport and Resistance Mechanisms
- Ferroptosis and cancer prognosis
- Obstructive Sleep Apnea Research
- Protease and Inhibitor Mechanisms
- Kruppel-like factors research
- Cancer Mechanisms and Therapy
- Angiogenesis and VEGF in Cancer
- Ear and Head Tumors
Shandong University
2014-2025
Qilu Hospital of Shandong University
2015-2025
The University of Texas MD Anderson Cancer Center
2010-2016
Baylor College of Medicine
2010
Circular RNAs (circRNAs), a novel class of endogenous noncoding RNAs, have been shown to important roles in number diseases, including several types cancers. We hypothesized that circRNAs are involved the pathogenesis hypopharyngeal squamous cell carcinoma (HSCC). To test our hypothesis, we initially compared expression profiles 4 paired HSCC and adjacent normal tissue samples by using circRNA microarray. The microarray data showed 2392 circRNAs, 1304 upregulated 1088 downregulated...
Researchers have shown that long noncoding RNAs are closely associated with the pathogenesis of laryngeal squamous cell carcinoma (LSCC). However, role RNA taurine-upregulated gene 1 (TUG1) in LSCC remains unclear, although it is recognized as an oncogenic regulator for several types carcinoma.qRT-PCR was performed to measure expression TUG1 tissues and lines. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) used effect on proliferation. Transwell assay flow cytometry...
Hypopharyngeal squamous cell carcinoma (HSCC) is a kind of head and neck (HNSCC) with poor prognosis. Metabolic reprogramming may regulate the tumor microenvironment (TME) by adapting quickly to cellular stress regulating immune response, but its role in HSCC has not been reported. We used nCounter ® Pathways Panel investigate metabolic reprogramming, stress, their relationship tissues adjacent normal tissues. Metabolism-related pathways nucleotide synthesis glycolysis were significantly...
// Jieyu Zhou 1, * Maocai Li Wenbin Yu 2, Wenming 1 , Juan Wang Xuan Xiang Guojun 3 Xinliang Pan Dapeng Lei Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key Laboratory Otolaryngology, Ministry Health, Shandong, P. R. China 2 Carcinogenesis and Translational Research (Ministry Education/Beijing), Head Neck surgery, Peking University Cancer Hospital & Institute, Beijing, Surgery, The Texas MD Anderson Center, Houston, TX, USA These authors contributed equally to this...
Head and neck squamous cell carcinoma (HNSC) is the most common malignant tumor of head neck. Due to insidious nature HNSC lack effective early diagnostic indicators, development novel biomarkers improve patient prognosis particularly urgent. In this study, we explored validated correlation between cytochrome P450 family 4 subfamily F member 12 (CYP4F12) expression levels progression using data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) datasets collected samples. We...
Human hypopharygeal squamous cell carcinoma (HSCC) is a common head and neck cancer with poor prognosis in advanced stages. The occurrence development of tumor the result mutual influence co-evolution between cells microenvironment (TME). Tumor immune (TIME) refers to surrounding cells. Studying TIME HSCC could provide new targets therapeutic strategies for HSCC.We performed single-cell RNA sequencing (scRNA-seq) analysis hypopharyngeal carcinoma, paracancerous, lymphoid tissues from five...
Hypopharyngeal squamous cell carcinoma (HSCC) remains one of the most lethal malignancies in head and neck. Long noncoding RNA (lncRNA) HOXA11-AS is proven to function as an oncogene a therapeutic target various tumors. Our previous study others have demonstrated that upregulated lncRNAs HSCC. However, role HSCC has not yet been identified. The current expression was significantly tumors positively associated with lymph node metastasis. Moreover, functional experiments revealed knockdown...
Abstract Hypopharyngeal squamous cell carcinoma (HSCC) accounts 95% of hypopharyngeal cancer, which is characterized by high early metastasis rate and poor prognosis. It reported that circular RNA involved in the occurrence development cancer; however, role circRNA cancer has little been investigated. We performed microarray qRT-PCR verification. The results showed circ_0058106 expression level was significantly upregulated tumor tissues than corresponding normal tissues. found upregulation...
Abstract Background: Single-nucleotide polymorphisms in the promoter region of FAS and FASLG may alter transcriptional activity these genes. We therefore investigated association between risk for second primary malignancy (SPM) after index squamous cell carcinoma head neck (SCCHN). Methods: used log-rank test Cox proportional hazard models to assess four single-nucleotide (FAS -1377 G > A, -670 A G, -844 C T, -124 G) with SPM-free survival SPM among 1,286 incident SCCHN patients....
Radioresistance remains a major problem in the treatment of patients with hypopharyngeal squamous cell carcinoma (HSCC). Long noncoding RNAs (lncRNAs) have important roles development, invasion, and metastasis various tumors, including HSCC, but little is known about role lncRNAs cancer radioresistance. The aim this study was to identify radioresistance-related mRNAs radioresistant (RS) subclone RS-FaDu cells. In study, we performed microarray analysis find differences time-course lncRNA...
Abstract Increasing number of circular RNAs (circRNAs) have been reported to play important role in gene regulation, carcinogenesis and pathogenesis various cancers. However, the biological functions underlying molecular mechanisms circRNAs hypopharyngeal squamous cell carcinoma (HSCC) remain elusive. Thus, secondary circRNA‐seq profiling was performed identify differentially expressed between HSCC tissues adjacent normal tissues, expression level circMATR3 (derived from human matrin3...
The PHLPP (pleckstrin homology [PH] domain leucine rich repeat protein phosphatase) family, which represents a family of novel Ser/Thr phosphatases, is composed 2 members: PHLPP1 and PHLPP2. PHLPPs partake in diverse cellular activities to exhibit their antitumor metastasis suppressor functions. It necessary investigate the expression patterns PHLPP2 hypopharyngeal squamous cell carcinomas (HSCCs) clarify clinical significance. A total 138 patients with primary HSCC who underwent curative...
<i>Purpose:</i> The aim of this study was to evaluate the suppression effect survivin shRNA on expression gene in human laryngeal cancer cell line Hep-2. <i>Procedures:</i> 60 cases squamous-cell carcinoma (LSCC) and 10 normal mucosa were examined using immunohistochemistry determine whether correlated with tumorigenesis. Three plasmid vectors short hairpin RNA (shRNA) specific for designed generated. Western blot real-time PCR analysis Hep-2 cells performed 48 h...
Abstract Since the prognosis of hypopharyngeal squamous cell carcinoma (HSCC) remains poor, identification miRNA as a potential prognostic biomarker for HSCC may help improve personalized therapy. In 2 cohorts with total 511 patients (discovery: N = 372 and validation: 139) after post‐operative radiotherapy, we used microarray qRT‐PCR to screen out significant miRNAs which might predict survival. Associations signature score these survival were performed by Kaplan‐Meier analysis multivariate...
Conclusion: These findings indicate that in hypopharyngeal squamous cell carcinoma (HSCC), hypermethylation and down-regulation of death-associated protein kinase-1 (DAPk1) are common events, which associated with a poor prognosis. Objectives: This study aimed to investigate the methylation expression DAPk1, tumor suppressor gene, HSCC, explore its clinical significance. Methods: The adjacent non-tumor tissues were collected from 53 patients HSCC. status DAPk1 was detected by...
FAS/FASL promoter variants are considered in altering transcriptional activity of those genes and consequently alter regulation cell death. However, no studies have investigated whether tumor sites contribute to the association between polymorphisms risk for second primary malignancy (SPM). In this study, FAS670 A > G, FAS1377 G A, FASL124 FASL844C T were genotyped 752 OPC 777 non-OPC patients. Both univariate multivariable cox proportional hazard models used assess associations. The...