A5100329174- Mesenchymal stem cell research
- Sirtuins and Resveratrol in Medicine
- Hair Growth and Disorders
- Telomeres, Telomerase, and Senescence
- Pluripotent Stem Cells Research
- Monoclonal and Polyclonal Antibodies Research
- Plant Molecular Biology Research
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- Cancer Mechanisms and Therapy
- Gout, Hyperuricemia, Uric Acid
- Inflammasome and immune disorders
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Radiopharmaceutical Chemistry and Applications
- Autophagy in Disease and Therapy
- Macrophage Migration Inhibitory Factor
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Liver physiology and pathology
- Alzheimer's disease research and treatments
Jilin Jianzhu University
2024-2025
International Peace Maternity & Child Health Hospital
2025
Shanghai Jiao Tong University
2025
Anhui Medical University
2023-2024
Ocean University of China
2023-2024
Jilin University
2014-2024
Shaanxi University of Chinese Medicine
2024
Baylor University
2024
Southern Medical University
2024
Air Force Medical University
2024
Mitochondrial damage is involved in the pathogenesis of osteoarthritis. Metformin, one most common prescriptions for patients with type 2 diabetes, can reportedly activate Sirtuin 3 (SIRT3) expression which protects mitochondria from oxidative stress. In this study, we investigated inhibitory property metformin on mitochondrial by focusing interleukin-1 beta (IL-1β)-stimulated osteoarthritis model using primary murine chondrocytes. Our results demonstrated that SIRT3 was downregulated...
Abstract Peptide hormones and neuropeptides are complex signaling molecules that predominately function through G protein-coupled receptors (GPCRs). Two unanswered questions remaining in the field of peptide-GPCR systems pertain to basis for diverse binding modes peptide ligands specificity protein coupling. Here, we report structures a neuropeptide, galanin, bound its receptors, GAL1R GAL2R, with their primary subtypes i q , respectively. The reveal unique pose which almost ‘lays flat’ on...
A scalable self-priming fractal branching microchannel net chip for digital PCR is developed the first time.
PBX homeobox 1 (PBX1) is involved in the maintenance of pluripotency human embryonic and hematopoietic stem cells; however, effects PBX1 self-renewal reprogramming hair follicle mesenchymal cells (HF-MSCs) are unclear. The AKT/glycogen synthase kinase (GSK) 3β pathway regulates cell metabolism, proliferation, apoptosis, reprogramming, p16 p21, which act downstream this pathway, regulate cycle, apoptosis induced by reprogramming. Here, we aimed to elucidate roles regulating proliferation...
Chondrocyte dysfunction is a key mechanism underlying osteoarthritis. Metformin has shown protective effects in many diseases. The present study aimed to investigate the of metformin on autophagy and apoptosis process A mouse osteoarthritis model was set up by surgically destabilizing medial meniscus knee. Intra-articular injection or vehicle applied right knee for eight weeks. Mouse articular chondrocytes were isolated passaged vitro experiments. Small interfering RNA (siRNA) transfection...
Chlorogenic acid (CGA) is a bioactive substance with anti-inflammatory activities. Clusters of CD36 have been suggested to be widely involved in inflammatory damage. However, the mechanism CGA protecting against LPS-induced inflammation involving regulation unclear. Here, we demonstrated that protected cell death and decreased production ROS. Moreover, SOD, CAT, GSH-Px activities were also upregulated CGA-treated cells during LPS stimulation. reduced COX-2 iNOS expression IL-1β, IL-6, TNF-α...
Hair loss, or alopecia, is a prevalent condition in modern society that imposes substantial mental and psychological burden on individuals. The types of hair include androgenetic alopecia areata, telogen effluvium; them, the most common condition. Traditional treatment modalities mainly involve medical options, such as minoxidil, finasteride surgical interventions, transplantation. However, these treatments still have many limitations. Therefore, exploring pathogenesis specifically focusing...
As the global population ages, incidence of elderly patients with dementia, represented by Alzheimer's disease (AD), will continue to increase. Previous studies have suggested that β-amyloid protein (Aβ) deposition is a key factor leading AD. However, clinical efficacy treating AD anti-Aβ antibodies not satisfactory, suggesting Aβ amyloidosis may be pathological change rather than Identification causes and development corresponding prevention treatment strategies an important goal current...
Reprogramming of somatic cells into inducible pluripotent stem (iPSCs) provides an alternative to using embryonic (ESCs). Mesenchymal derived from human hair follicles (hHF-MSCs) are easily accessible, reproducible by direct plucking hairs. Whether these hHF-MSCs can be reprogrammed has not been previously reported. Here we report the generation iPSCs obtained several were isolated follicle tissues and their mesenchymal nature confirmed detecting cell surface antigens multilineage...
Abstract Phase contrast time-lapse microscopy is a non-destructive technique that generates large volumes of image-based information to quantify the behaviour individual cells or cell populations. To guide development algorithms for computer-aided tracking and analysis, 48 image sequences, each spanning approximately 3.5 days, were generated with accompanying ground truths C2C12 myoblast cultured under 4 different media conditions, including fibroblast growth factor 2 (FGF2), bone...
Lanthanide metal clusters are composed of rigid multinuclear cores encapsulated by organic ligands, which have become one the most interesting research frontiers because their fantastic architecture, intriguing physical and chemical properties, potential applications. However, very little attention has been paid to exploring as highly efficient optical materials. Gd16 a new cluster structure that rich varied coordination environment, is conducive doping thus controlling luminescence color...
Abstract Background To determine the pathogenicity of a novel splicing variant in SMARCC2 gene identified from pair adult male monozygotic twins with neurodevelopmental disorder, and to investigate genotype-phenotype characteristics associated variants. Methods Whole-exome sequencing (WES) was conducted on proband, candidate variants were validated using Sanger within family. The effect mRNA processing analyzed reverse transcription PCR (RT-PCR) TA-clone samples derived proband. clinical...
We propose a novel deep learning segmentation algorithm (DSRF) based on multi-scale supervision and residual feedback strategy for precise of the optic chiasm nerves in CT images nasopharyngeal carcinoma (NPC) patients. collected 212 NPC their ground truth labels from SegRap2023, StructSeg2019 HaN-Seg2023 datasets. Based hybrid pooling strategy, we designed decoder (HPS) to reduce small organ feature loss during convolutional neural networks. This uses adaptive average refine high-level...
Stem cells derived from elderly donors or harvested by repeated subculture exhibit a marked decrease in proliferative capacity and multipotency, which not only compromises their therapeutic potential but also raises safety concerns for regenerative medicine. NANOG—a well-known core transcription factor—plays an important role maintaining the self-renewal pluripotency of stem cells. Unfortunately, mechanism that NANOG delays mesenchymal cell (MSC) senescence is until now. In our study, we...
Three nonviral gene vectors, TPA-BI-A/B/C, have been designed and synthesized by the combination of one or two hydrophilic [12]aneN3 moieties two-photon fluorescent triphenylamine-benzylideneimidazolone (TPA-BI) units through different ester linkage. Spectroscopic characterization demonstrated that TPA-BI-A/B/C had strong aggregation-induced emissions (AIE), large Stokes shifts (230, 284, 263 nm), absorption cross sections (δ2PA) (67, 592, 80 GM). Gel electrophoresis indicated three...
Stem cell senescence and depletion are major causes of aging aging-related diseases. The NAD (Nicotinamide adenine dinucleotide) - SIRT1 (Silent Information Regulator 1) PARP1 (Poly (ADP-ribose) polymerase-1) axis has gained interest owing to its significant role in regulating stem organismal aging. A recent study from our lab showed that pre-B-cell leukemia transcription factor1 (PBX1) overexpression attenuates hair follicle-derived mesenchymal cells (HF-MSCs) apoptosis by ROS-mediated DNA...