A5002863279- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Protist diversity and phylogeny
- DNA Repair Mechanisms
- Genetic and Kidney Cyst Diseases
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Advanced Thermodynamics and Statistical Mechanics
- Cell death mechanisms and regulation
- Cellular Mechanics and Interactions
- Protein Structure and Dynamics
- Crystallization and Solubility Studies
- Enzyme Structure and Function
- Nonlinear Dynamics and Pattern Formation
- Legume Nitrogen Fixing Symbiosis
- X-ray Diffraction in Crystallography
- Cancer-related gene regulation
- Characterization and Applications of Magnetic Nanoparticles
- Advanced Materials and Mechanics
- Organometallic Complex Synthesis and Catalysis
- HIV/AIDS Research and Interventions
- Cancer-related Molecular Pathways
- Micro and Nano Robotics
- Mitochondrial Function and Pathology
Yale University
2020-2025
Fudan University Shanghai Cancer Center
2013-2021
Shanghai Medical College of Fudan University
2013-2021
Abstract Thousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Coordination among multiple OADs is essential for generating mechanical forces bend microtubule doublets (MTDs). Using electron microscopy, we determined high-resolution structures Tetrahymena thermophila OAD arrays bound MTDs two different states. preferentially binds MTD protofilaments with pattern resembling native tracks its distinct microtubule-binding domains. Upon binding, free...
Abstract Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival development of nearly all eukaryotic species. An essential regulator conserved central apparatus (CA) motile cilia, composed a pair microtubules (C1 C2) associated with hundreds protein subunits per repeating unit. It largely unclear how CA plays its regulatory roles motility. Here, we present high-resolution structures Chlamydomonas reinhardtii by...
Revealing high-resolution structures of microtubule-associated proteins (MAPs) is critical for understanding their fundamental roles in various cellular activities, such as cell motility and intracellular cargo transport. Nevertheless, large flexible molecular motors that dynamically bind release microtubule networks are challenging cryo-electron microscopy (cryo-EM). Traditional structure determination MAPs bound to microtubules needs alignment information from the reconstruction...
Axonemal dynein assembly occurs in the cytoplasm and numerous cytosolic factors are specifically required for this process. Recently, one factor (DNAAF3/PF22) was identified as a methyltransferase. Examination of Chlamydomonas dyneins found they methylated at sub-stoichiometric levels on multiple sites, including Lys Arg residues several nucleotide binding domains microtubule-binding region. Given highly conserved nature axonemal dyneins, key question is whether methylation happens only from...
Revealing high-resolution structures of microtubule-associated proteins (MAPs) is critical for understanding their fundamental roles in various cellular activities, such as cell motility and intracellular cargo transport. Nevertheless, large molecular motors that dynamically bind release microtubule networks are challenging cryo-electron microscopy (cryo-EM). Traditional structure determination MAPs bound to microtubules needs alignment information from the reconstruction microtubules, which...
Abstract Cytoplasmic dynein-1, a microtubule-based motor protein, requires dynactin and an adaptor to form the processive dynein-dynactin-adaptor (DDA) complex. The role of microtubules in DDA assembly has been elusive. Here, we reveal detailed structural insights into microtubule-mediated using cryo-electron microscopy. We find that adaptor-independent dynein- complex (DD) predominantly forms on intrinsic 2:1 stoichiometry, induced by spontaneous parallelization dynein upon microtubule...
Thousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Coordination among multiple OADs is essential for generating mechanical forces bend microtubule doublets (MTDs). Using electron microscopy, we determined high-resolution structures OAD arrays bound MTD two different states. preferentially binds protofilaments with pattern resembling native tracks its distinct microtubule-binding domains. Upon binding, free induced adopt stable parallel...
Eukaryotic cilia are evolutionarily conserved organelles that responsible for cellular motility, sensory reception, embryonic development and intercellular communication. A typical motile cilium is characterized by its ʹ9 + 2ʹ scaffold, composed of nine microtubule doublets (MTDs), a central pair complex (CPC). Two rows axonemal dyneins, the outer-arm dynein (OAD) inner-arm (IAD), power ciliary beating sliding two adjacent MTDs. OAD key motor protein generates majority mechanical forces...
Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival development of nearly all eukaryotic species. An essential regulator conserved central apparatus (CA) motile cilia, composed a pair microtubules (C1 C2) associated with hundreds protein subunits per repeating unit. It largely unclear how CA plays its regulatory roles motility. Here, we present high-resolution structures Chlamydomonas reinhardtii by cryo-electron...