A5074294571- Calcium signaling and nucleotide metabolism
- Ion Channels and Receptors
- Cellular transport and secretion
- Adenosine and Purinergic Signaling
- Ion channel regulation and function
- Semiconductor materials and interfaces
- Semiconductor materials and devices
- Neuroscience and Neuropharmacology Research
- Autophagy in Disease and Therapy
- Piperaceae Chemical and Biological Studies
- Plant Molecular Biology Research
- Copper Interconnects and Reliability
- Pain Mechanisms and Treatments
- Pineapple and bromelain studies
- Neonatal Health and Biochemistry
- Metal and Thin Film Mechanics
- ZnO doping and properties
- Pancreatic function and diabetes
- Advanced Memory and Neural Computing
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
Dalhousie University
2015-2024
Shanghai Jiao Tong University
2002-2022
Chinese National Human Genome Center at Shanghai
2022
Anhui Medical University
2019
Shanghai University of Medicine and Health Sciences
2018
University of Michigan
2007-2012
Augusta University
2006-2008
Howard Hughes Medical Institute
2007
Harvard University
2007
Augusta University Health
2007
Mammalian two-pore channel proteins (TPC1, TPC2; TPCN1, TPCN2) encode ion channels in intracellular endosomes and lysosomes were proposed to mediate endolysosomal calcium release triggered by the second messenger, nicotinic acid adenine dinucleotide phosphate (NAADP). By directly recording TPCs endolysosomes from wild-type TPC double-knockout mice, here we show that, contrast previous conclusions, are fact sodium-selective activated PI(3,5)P2 not NAADP. Moreover, primary is Na+, K+, as had...
Transient receptor potential (TRP) genes of the mucolipin subfamily (TRPML1–3 and MCOLN1–3) are presumed to encode ion channel proteins intracellular endosomes lysosomes. Mutations in human TRPML1 (mucolipin 1/MCOLN1) result mucolipidosis type IV, a severe inherited neurodegenerative disease associated with defective lysosomal biogenesis trafficking. A mutation mouse TRPML3 (A419P; Va ) results varitint–waddler ( phenotype. mice deaf, exhibit circling behavior due vestibular defects, have...
Intra-endolysosomal Ca(2+) release is required for endolysosomal membrane fusion with intracellular organelles. However, the molecular mechanisms intra-endolysosomal and downstream targets involved in remain elusive. Previously, we demonstrated that P2X4 forms channels activated by luminal adenosine triphosphate a pH-dependent manner. In this paper, show overexpression of P2X4, as well increasing activity alkalinization endolysosome lumen, promoted vacuole enlargement cells cell-free assay....
The mucolipin TRP (TRPML) proteins are a family of endolysosomal cation channels with genetically established importance in humans and rodent. Mutations human TRPML1 cause type IV mucolipidosis, devastating pediatric neurodegenerative disease. Our recent electrophysiological studies revealed that, although TRPML1-mediated current can only be recorded late endosome lysosome (LEL) using the patch clamp technique, proline substitution (TRPML1(V432P)) results large whole cell current. Thus, it...
Macroautophagy/autophagy is an evolutionarily conserved pathway that required for cellular homeostasis, growth and survival. The lysosome plays essential role in autophagy regulation. For example, the activity of MTORC1, a master regulator autophagy, regulated by nutrients within lysosome. Starvation inhibits MTORC1 causing induction. Given critical protein synthesis feedback regulatory mechanism must exist to restore during starvation. However, molecular underlying this regulation unclear....
Agrin is believed to be a factor used by motoneurons direct acetylcholine receptor (AChR) clustering at the neuromuscular junction. However, exactly how agrin mediates this effect remains unclear. Here we demonstrate that β-catenin interacts with rapsyn, molecule key for AChR clustering. stimulation increases association of surface AChRs. Suppression expression inhibited agrin-induced clustering, suggesting necessary role in event. The action did not appear require function T-cell factors...
Lysosomes contain abundant ATP, which is released through lysosomal exocytosis following exposure to various stimuli. However, the molecular mechanisms underlying ATP accumulation remain unknown. The vesicular nucleotide transporter, also known as solute carrier family 17 member 9 (SLC17A9), has been shown function in transport across secretory vesicles/granules membrane adrenal chromaffin cells, T and pancreatic cells. Here, using mammalian cell lines, we report that SLC17A9 highly enriched...
Macrophages are highly specialized in removing large particles including dead cells and cellular debris. When stimulated, delivery of the intracellular lysosomal membranes is required for formation plasmalemmal pseudopods phagosomes. As a key Ca2+ channel, Transient Receptor Potential Mucolipin-1 (TRPML1) regulates exocytosis subsequent phagosome biogenesis, thereby promoting phagocytosis extracellular particles. Recently, we have suggested that TRPML1-mediated essentially dependent on big...