A5056981580- Inflammatory Bowel Disease
- Cancer Genomics and Diagnostics
- Microscopic Colitis
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Gastrointestinal disorders and treatments
- Genetic factors in colorectal cancer
- Liver Diseases and Immunity
- Pediatric Hepatobiliary Diseases and Treatments
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- Radiomics and Machine Learning in Medical Imaging
- Digestive system and related health
- Liver Disease Diagnosis and Treatment
- Eosinophilic Esophagitis
- Gastrointestinal Tumor Research and Treatment
- Gallbladder and Bile Duct Disorders
- Cancer Cells and Metastasis
- Lymphoma Diagnosis and Treatment
- Genetics and Neurodevelopmental Disorders
- DNA Repair Mechanisms
- Asthma and respiratory diseases
- Cancer, Hypoxia, and Metabolism
Hospital for Sick Children
2016-2025
University of Toronto
2015-2024
McGill University Health Centre
2023-2024
McGill University
2023
SickKids Foundation
2017-2022
Fraunhofer Institute for Toxicology and Experimental Medicine
2022
Princess Margaret Cancer Centre
2016
University Health Network
2016
Western University
2006-2013
King Fahd Medical City
2012
Anaplastic thyroid carcinoma is arguably the most lethal human malignancy. It often co-occurs with differentiated cancers, yet molecular origins of its aggressivity are unknown. We sequenced tumor DNA from 329 regions cancer, including 213 patients primary anaplastic carcinomas. also whole genome 9 using multi-region sequencing both and cancer components. Using these data, we demonstrate thatanaplastic carcinomas have a higher burden mutations than other distinct mutational signatures...
Abstract Imaging mass cytometry was used for direct visualization of platinum localization in tissue sections from tumor and normal tissues cisplatin-treated mice bearing pancreas cancer patient-derived xenografts. This recently-developed technology enabled simultaneous detection multiple markers to define cell lineage, DNA damage response, proliferation functional state, providing a highly detailed view drug incorporation at the cellular level. A striking unanticipated finding extensive...
Background & AimsA proportion of infants and young children with inflammatory bowel diseases (IBDs) have subtypes associated a single gene variant (monogenic IBD). We aimed to determine the prevalence monogenic disease in cohort pediatric patients IBD.MethodsWe performed whole-exome sequencing analyses blood samples from an unselected 1005 IBD, aged 0–18 years (median age at diagnosis, 11.96 years) center Canada their family members (2305 total). Variants believed cause IBD were validated...
Increasingly, spasticity is managed with surgically implanted Intrathecal Baclofen pumps. pump revision surgery unrelated to programmable end-of-life not uncommon, requiring special attention during pre-, intra-, and postoperative management. We aimed identify describe complications of as well report avoidance management complications. Methods Materials. Through 2002-2006, at the department neurosurgery, Henry Ford Oakwood Health Systems, pumps were in 44 patients: 24 children versus 20...
Background & AimsMutations in the tetratricopeptide repeat domain 7A gene (TTC7A) cause intestinal epithelial and immune defects. Patients can become deficient develop apoptotic enterocolitis, multiple atresia, recurrent stenosis. The disease patients with TTC7A deficiency is severe untreatable, it recurs despite resection or allogeneic hematopoietic stem cell transplant. We screened drugs for those that prevent apoptosis of cells tested their effects an animal model disease.MethodsWe...
Background An epidemic of human papillomavirus (HPV)-related oropharyngeal squamous cell cancer (OPSCC) has been reported worldwide largely due to oral infection with HPV type-16, which is responsible for approximately 90% HPV-positive cases. The purpose this study was determine the rate in Southwestern Ontario, Canada. Methods A retrospective search identified ninety-five patients diagnosed OPSCC. Pre-treatment biopsy specimens were tested p16 expression using immunohistochemistry and...
β-arrestins play critical roles in chemotaxis and cytoskeletal reorganization downstream of several receptor types, including G protein-coupled receptors (GPCRs), which are targets for greater than 50% all pharmaceuticals. Among them, lysophosphatidic acid (LPA), namely LPA1 overexpressed breast cancer promote metastatic spread. We have recently reported that β-arrestin2 regulates LPA1-mediated cell migration invasion, although the underlying molecular mechanisms not clearly understood. show...
Abstract Background The co-occurrence of inflammatory bowel disease (IBD) and adenomatous polyposis syndrome is uncommon. Aims To highlight the challenges diagnosis management a rare case concurrent polyps in child with newly diagnosed Crohn’s disease. Methods Review patient charts, endoscopic histopathology images from electronic medical records. Results An 11-year-old boy Korean origin presented 6-month history periumbilical abdominal pain, diarrhea, 5 kg weight loss, perianal abscess,...
The pediatric inflammatory bowel disease (PIBD) classes algorithm was developed to bring consistency labelling of colonic IBD, but labels are exclusively based on features atypical for ulcerative colitis (UC).The aim the study develop an and identify that discriminate between UC Crohn (CD).Baseline clinical, endoscopic, radiologic, histologic data, including PIBD class in 74 IBD (56: UC, 18: CD) patients were collected. additional common used perform initial clustering, using similarity...
Abstract We report a case of Mismatch Repair Deficiency (MMRD) caused by germline homozygous EPCAM deletion leading to tissue-specific loss MSH2. Through the use patient-derived cells and organoid technologies, we performed stepwise in vitro differentiation colonic brain organoids from reprogrammed del iPSC derived patient fibroblasts. Differentiation epithelial-colonic exhibited continuous increased expression hypermethylation MSH2 promoter. This was associated with expression, mutational...
Local disease control is a major challenge in pancreatic cancer treatment, because surgical resection of the primary tumor only possible minority patients and radiotherapy cannot be delivered curative doses. Despite promise photothermal therapy (PTT) for focal ablation tumors, this approach remains underinvestigated. Using sensitizers combination with laser light irradiation PTT can result more efficient conversion energy to heat improved spatial confinement thermal destruction tumor....
Hypoxia, the state of low oxygenation that often arises in solid tumours due to their high metabolism and irregular vasculature, is a major contributor resistance radiation therapy (RT) other treatments. Conventional RT extends treatment over several weeks or more, nominally allows time for oxygen levels increase ("reoxygenation") as cancer cells are killed by RT, mitigating impact hypoxia. Recent advances have led an use stereotactic body radiotherapy (SBRT), which delivers doses five fewer...
Wilson disease (WD) has diverse presentations that frequently mimic other liver diseases. Distinguishing WD from non-alcoholic fatty (NAFLD) and autoimmune hepatitis (AIH), can be difficult critical implications for medical management. This study aimed to examine the utility of histological features in children compared those with NAFLD AIH.A review biopsy slides was performed a clinical and/or genetic diagnosis WD, seen at Hospital Sick Children between 1981 2019 controls AIH. 37 (20 NAFLD;...
Abstract Background Accurate identification of inflammatory cells from mucosal histopathology images is important in diagnosing ulcerative colitis. The eosinophils the colonic mucosa has been associated with disease course. Cell counting not only time-consuming but can also be subjective to human biases. In this study we developed an automatic eosinophilic cell tool images, using deep learning. Method Four pediatric IBD pathologists two North American hospitals annotated 530 crops 143...