Christoph Klein
A5103124036- Immunodeficiency and Autoimmune Disorders
- Blood disorders and treatments
- Cardiac Imaging and Diagnostics
- Immune Cell Function and Interaction
- Advanced MRI Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Inflammatory Bowel Disease
- CAR-T cell therapy research
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Viral-associated cancers and disorders
- Cell Adhesion Molecules Research
- Autoimmune and Inflammatory Disorders Research
- Cardiovascular Function and Risk Factors
- Lymphoma Diagnosis and Treatment
- IL-33, ST2, and ILC Pathways
- Cellular Mechanics and Interactions
- Neutropenia and Cancer Infections
- Cytomegalovirus and herpesvirus research
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Polyomavirus and related diseases
- Pluripotent Stem Cells Research
- Chronic Lymphocytic Leukemia Research
Ludwig-Maximilians-Universität München
2016-2025
Charité - Universitätsmedizin Berlin
1999-2025
Deutsches Herzzentrum der Charité
1998-2025
LMU Klinikum
2018-2024
Humboldt-Universität zu Berlin
1999-2024
München Klinik
2012-2024
Freie Universität Berlin
2024
Deutsches Herzzentrum München
2002-2024
German Center for Pediatric and Adolescent Rheumatology
2024
University Hospital Münster
2024
We performed genetic-linkage analysis and candidate-gene sequencing on samples from two unrelated consanguineous families with children who were affected by early-onset inflammatory bowel disease. screened six additional patients colitis for mutations in candidate genes carried out functional assays patients' peripheral-blood mononuclear cells. an allogeneic hematopoietic stem-cell transplantation one patient.
Abstract We report the updated classification of Inborn Errors Immunity/Primary Immunodeficiencies, compiled by International Union Immunological Societies Expert Committee. This documents key clinical and laboratory features 430 inborn errors immunity, including 64 gene defects that have either been discovered in past 2 years since previous update (published January 2018) or were characterized earlier but confirmed expanded upon subsequent studies. The application next-generation sequencing...
Abstract The Human Phenotype Ontology (HPO, https://hpo.jax.org) was launched in 2008 to provide a comprehensive logical standard describe and computationally analyze phenotypic abnormalities found human disease. HPO is now worldwide for phenotype exchange. has grown steadily since its inception due considerable contributions from clinical experts researchers diverse range of disciplines. Here, we present recent major extensions the neurology, nephrology, immunology, pulmonology, newborn...
Abstract We report the updated classification of inborn errors immunity, compiled by International Union Immunological Societies Expert Committee. This documents key clinical and laboratory features 55 novel monogenic gene defects, 1 phenocopy due to autoantibodies, that have either been discovered since previous update (published January 2020) or were characterized earlier but confirmed expanded in subsequent studies. While variants additional genes associated with immune diseases reported...
Background —The analysis of wall motion abnormalities with dobutamine stress echocardiography (DSE) is an established method for the detection myocardial ischemia. With ultrafast magnetic resonance tomography, identical protocols as used can be applied. Methods and Results —In 208 consecutive patients (147 men, 61 women) suspected coronary artery disease, DSE harmonic imaging (DSMR) (1.5 T) were performed before cardiac catheterization. DSMR images acquired during short breath-holds in 3...
Beginning in 1970, a committee was constituted under the auspices of World Health Organization (WHO) to catalog primary immunodeficiencies. Twenty years later, International Union Immunological Societies (IUIS) took remit this committee. The current report details categorization and listing 354 (as February 2017) inborn errors immunity. growth increasing complexity field have been impressive, encompassing an variety conditions, classification described here will serve as critical reference...
We report the updated classification of primary immunodeficiency diseases, compiled by ad hoc Expert Committee International Union Immunological Societies. As compared to previous edition, more than 15 novel disease entities have been added in version. For each disorders, key clinical and laboratory features are provided. This is meant help diagnostic approach patients with these diseases.
Background — Recent studies indicate that MRI, after administration of gadolinium-diethylenetriamine pentaacetic acid, can identify nonviable areas in dysfunctional myocardium. We compared MRI hyperenhancement with PET as a gold standard for detection and quantification myocardial scar tissue. Methods Results Thirty-one patients ischemic heart failure (ejection fraction, 28±9%) were imaged MRI. Scar was defined regionally increased signal intensity 20 minutes injection 0.2 mmol/kg acid...
With MRI, an index of myocardial perfusion reserve (MPRI) can be determined. We assessed the value this technique for noninvasive detection coronary artery disease (CAD) in patients with suspected CAD.Eighty-four referred a primary diagnostic angiography were examined 1.5 T MRI tomograph (Philips-ACS). For each heartbeat, 5 slices acquired during first pass 0.025 mmol gadolinium-diethylenetriamine pentaacetic acid/kg body weight before and adenosine vasodilation by using turbo-gradient...
Abstract Since 2013, the International Union of Immunological Societies (IUIS) expert committee (EC) on Inborn Errors Immunity (IEI) has published an updated phenotypic classification IEI, which accompanies and complements their genotypic into ten tables. This is user-friendly serves as a resource for clinicians at bedside. There are now 430 single-gene IEI underlying phenotypes diverse infection, malignancy, allergy, autoimmunity, autoinflammation. We herein report 2019 classification,...
Myocardial perfusion reserve can be noninvasively assessed with cardiovascular MR. In this study, the diagnostic accuracy of technique for detection significant coronary artery stenosis was evaluated.In 15 patients single-vessel disease and 5 without disease, signal intensity-time curves first pass a gadolinium-DTPA bolus injected through central vein catheter were evaluated before after dipyridamole infusion to validate technique. A linear fit used determine upslope, cutoff value...
The Wiskott-Aldrich syndrome (WAS) is an X-linked recessive primary immunodeficiency disorder associated with thrombocytopenia, eczema, and autoimmunity. We treated two patients who had this a transfusion of autologous, genetically modified hematopoietic stem cells (HSC). found sustained expression WAS protein in HSC, lymphoid myeloid cells, platelets after gene therapy. T B natural killer (NK) monocytes were functionally corrected. After treatment, the patients' clinical condition markedly...
Wiskott-Aldrich syndrome gene therapy is feasible, but γ-retroviral vectors contribute a substantial risk of leukemogenesis.
Since the 1990s, International Union of Immunological Societies (IUIS) PID expert committee (EC), now called Inborn Errors Immunity Committee, has published every other year a classification inborn errors immunity. This complete catalog serves as reference for immunologists and researchers worldwide. However, it was unadapted clinicians at bedside. For those, IUIS EC is publishing phenotypical since 2013, which proved to be more user-friendly. There are 320 single-gene immunity underlying...
The main features of severe congenital neutropenia are the onset bacterial infections early in life, a paucity mature neutrophils, and an increased risk leukemia. In many patients, genetic causes unknown.We performed genomewide genotyping linkage analysis on two consanguineous pedigrees with total five children affected neutropenia. Candidate genes from interval were sequenced. Functional assays reconstitution experiments carried out.All index patients susceptible to had very few neutrophils...
Autosomal recessive, complete TYK2 deficiency was previously described in a patient (P1) with intracellular bacterial and viral infections features of hyper-IgE syndrome (HIES), including atopic dermatitis, high serum IgE levels, staphylococcal abscesses. We identified seven other TYK2-deficient patients from five families four different ethnic groups. These were homozygous for one null mutations, that seen P1. They displayed mycobacterial and/or infections, but no HIES. All eight impaired...