Phycocyanin, a type of functional food colorant, is shown to have potent anti-cancer property. Non-small cell lung cancer (NSCLC) one the most aggressive form cancers with few effective therapeutic options. Previous studies demonstrated that phycocyanin exerts growth inhibitory effect on NSCLC A549 cells. However, its biological function and underlying regulatory mechanism other cells still remain unknown. Here, we investigated in vitro three typical lines, NCI-H1299, NCI-H460, LTEP-A2, for...

Biogenic amines produced during fermentation are harmful and deteriorate the sensory experience of food. However, methods for overcoming impact biogenic in Huangjiu, a traditional Chinese food, have not been considered research. This review analyzes various studies that evaluated content amine formation amines, associated factors. Furthermore, study discusses health hazards summarizes prevalent approaches to determine their Huangjiu. Finally, strategies reduce Huangjiu listed. Although...

Phycocyanin (PC), derived from cyanobacteria and Spirulina cells, is a type of natural antineoplastic marine protein. It has been reported that phycocyanin exerts an antitumor function in non-small cell lung cancer (NSCLC) but the underlying mechanism not elucidated. In this research, transcriptome study was performed to investigate regulatory mechanisms on human NSCLC A549 cells. The survival rate proliferation ability cells were markedly reduced by phycocyanin, along with abnormal...

Recently, the NLRP3 inflammasome activation in eyes has been known to be associated with pathogenesis of age-related macular degeneration. The aim this study was investigate protective effects cyanidin-3-glucoside (C3G), an important anthocyanin great potential for preventing eye diseases, against 4-hydroxyhexenal- (HHE-) induced inflammatory damages human retinal pigment epithelial cells, ARPE-19. We noticed that C3G pretreatment ARPE-19 cells rescued HHE-induced antiproliferative effects....

Phycocyanin is a type of marine functional food additive, exerting health care efficacy with no side effects. It has been shown that phycocyanin possesses anticancer function in non-small cell lung cancer (NSCLC) cells, but the underlying regulatory mechanism still remains unclear. Further investigation on antineoplastic would provide useful information NSCLC treatment. In this study, we explored vitro and three typical lines, H1975, H1650, LTEP-a2, for first time. Phenotypic experiments...

As a type of functional food additive, phycocyanin is shown to have potential antineoplastic property. However, its underlying anticancer mechanism in melanoma cells remains unknown. We previously reported 35S vivo/vitro labeling analysis for dynamic proteomic (SiLAD) technology. It could exclusively detect protein synthesis rates via pulse newly expressed proteins by 35S, providing high time-resolution method variations. In the present study, we performed time course A375 after treatment...

Phycocyanin, derived from Spirulina platensis, is a type of natural antineoplastic marine protein. It known that phycocyanin exerts anticancer effects on non-small-cell lung cancer (NSCLC) cells, but its underlying mechanism has not been elucidated. Herein, the antitumor function and regulatory were investigated in three NSCLC cell lines for first time: H358, H1650, LTEP-a2. Cell phenotype experiments suggested could suppress survival rate, proliferation, colony formation, migration...

Histone acetyltransferase (HAT) has been reported to be pivotal for various physiological processes in many fungi. However, the functions that HAT Rtt109 perform edible fungi Monascus and underlying mechanism remains unclear. Here, we identified rtt109 gene Monascus, constructed knockout strain (Δrtt109) its complementary (Δrtt109:com) by CRISPR/Cas9 methods, functionally characterized roles play Monascus. Deletion of significantly reduced conidia formation colony growth, whereas, it...

Huangjiu usually caused rapid-drunkenness and components such as β-benzyl ethanol (β-be), isopentanol (Iso), histamine (His), phenethylamine (PEA) have been reported linked with intoxication. However, the destructive effect of these on gut microbiota liver is unclear. In this study, we found oral treatment components, especially His, stimulated level oxidative stress inflammatory cytokines in serum mice. The community was changed lipopolysaccharide (LPS) increased significantly....

Polycystic ovary syndrome (PCOS) is a common endocrine disorder, which characterized by hyperandrogenism. Polyunsaturated fatty acids (PUFAs) are necessary for the body's metabolism, growth and development. Despite well-known benefits of omega-3 polyunsaturated acid supplementation on modulation PCOS ovarian function, relatively little known about precise regulation mechanism. The objective this study was to determine cellular molecular mechanisms regulates CYP51 expression steroid...

Hydroxytyrosol, a valuable plant-derived phenolic compound, is increasingly produced from microbial fermentation. However, the promiscuity of key enzyme HpaBC, two-component flavin-dependent monooxygenase Escherichia coli, often leads to low yields. To address this limitation, we developed novel strategy utilizing consortia catalysis for hydroxytyrosol production. We designed biosynthetic pathway using tyrosine as substrate and selected enzymes overexpressing glutamate dehydrogenase GdhA...

When Monascus purpureus was co-cultured with Saccharomyces cerevisiae, we noted significant changes in the secondary metabolism and morphological development of Monascus. In yeast co-culture, although pH not different from that a control, mycelial biomass increased during fermentation, Monacolin K yield significantly enhanced (up to 58.87% higher). However, pigment production did increase. Co-culture S. cerevisiae expression levels genes related (mokA-mokI), especially mokE, mokF, mokG....

Monascus is widely used in Asia and produces active metabolites–Monascus pigments monacolin K. Quorum sensing (QS) regulates mycelial development secondary metabolism filamentous fungi; however, QS studies are limited. Here, the effect of γ-butyrolactone, a possible molecule, on purpureus was investigated. γ-butyrolactone accelerated cell growth, promoted synthesis K, reduced citrinin synthesis. Scanning electron microscopy showed caused surface folding spore pitting. Real-time quantitative...

The hydroalcoholic extract of sea buckthorn (Hippophae rhamnoides L.) seed residues (HYD-SBSR) is a potential skin whitening agent. To test this material as agent, we identified and quantified the main chemical constituents HYD-SBSR by using ultra-performance liquid chromatography coupled with quadruple time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) ultra-high performance triple (UPLC-QQQ-MS). anti-melanogenesis properties on B16F10 mouse melanoma cells were analysed mechanism was...

Phycocyanin, derived from marine algae, is known to have noteworthy antineoplastic properties. However, the underlying mechanism involved in phycocyanin-mediated anti-growth function on non-small cell lung cancer (NSCLC) cells still ambiguous. Here, we investigated of action phycocyanin H1299, A549, and LTEP-a2 cells. According results obtained, insulin receptor substrate 1 (IRS-1) expression was reduced by phycocyanin. Cell phenotype tests showed that siRNA knockdown IRS-1 significantly...

Non-small cell lung cancer (NSCLC) remains a leading cause of mortality worldwide. Phycocyanin, type marine food additives, has been reported to exert antineoplastic effects in NSCLC. The aim this study was investigate roles autophagy the anticancer process phycocyanin Results showed that increased LC3-II/LC3-I ratio and decreased p62 level NSCLC cells dose- time-dependent manner. Laser scanning confocal observation autophagic LC3 puncta promotes flux cells. In addition, AMPK signaling...

Glochidion ellipticum Wight is a medicinal plant, rich in polyphenols, frequently used by the indigenous communities of Bangladesh and possess with multiple health benefits. It exerts anti-inflammatory antidiarrheal properties, but detailed chemical constituents are yet to be elucidated.Glochidion extracts were analyzed using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry then tested both lipopolysaccharide (LPS) induced inflammation Raw 264.7...

The translationally controlled tumor protein (TCTP) has been reported to promote progression of many physiological processes. However, whether TCTP is involved in liver regeneration rarely studied. This study aimed investigate the potential role serum after two-thirds partial hepatectomy.The synthesis rate and accumulated expression was assessed by phosphor imaging Western blot analysis, respectively. mRNA tctp analyzed quantitative real-time PCR. effect on hepatocyte proliferation...

Phycocyanin, a type of functional food additive, is shown to exert antineoplastic property in non-small cell lung cancer (NSCLC). Our previous work demonstrated that phycocyanin could suppress the viability NSCLC cells via regulating receptor-interacting serine/threonine-protein kinase 1 (RIPK1), but underlying mechanism remains unknown. Herein, high-throughput miRNA sequencing method was employed further explore variations for first time. 43 down-regulated and 18 up-regulated miRNAs...