A5101415246- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Renal and related cancers
- RNA Research and Splicing
- Intracerebral and Subarachnoid Hemorrhage Research
- Acute Ischemic Stroke Management
- Dialysis and Renal Disease Management
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Bioactive Compounds and Antitumor Agents
- Peptidase Inhibition and Analysis
- Pancreatitis Pathology and Treatment
- Hypothalamic control of reproductive hormones
- Astro and Planetary Science
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- Lipid metabolism and biosynthesis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Hemophilia Treatment and Research
- Cancer-related gene regulation
- Signaling Pathways in Disease
- Nuclear Structure and Function
- Reproductive Physiology in Livestock
- Neurosurgical Procedures and Complications
Augusta University
2010-2024
Shandong Agricultural University
2013-2024
Fujian Medical University
2024
Fujian Provincial Hospital
2024
Chongqing Dazu District People's Hospital
2022
China Academy of Space Technology
2021
China Agricultural University
2015-2020
China Pharmaceutical University
2019
State Key Laboratory of Natural Medicine
2019
Nankai University
2018
Abstract Spatially ordered embryo-like structures self-assembled from blastocyst-derived stem cells can be generated to mimic embryogenesis in vitro. However, the assembly system and developmental potential of such needs further studied. Here, we devise a nonadherent-suspension-shaking generate (ETX-embryoids) using mouse embryonic, trophoblast extra-embryonic endoderm cells. When cultured together, three cell types aggregate sort into lineage-specific compartments. Signaling among these...
Preimplantation embryos undergo zygotic genome activation and lineage specification resulting in three distinct cell types the late blastocyst. The molecular mechanisms underlying this progress are largely unknown bovines. Here, we sought to analyze an extensive set of regulators at single-cell level define events involved development bovine Using a quantitative microfluidics approach single cells, analyzed mRNA levels 96 genes known function early embryonic maintenance stem pluripotency...
Matrix metalloproteinases (MMPs) are a specific class of proteolytic enzymes that play critical roles in follicular development and luteinization mammals. However, the role MMPs avian ovary remains largely unknown. We found three MMP genes (MMP1, MMP3, MMP9) were significantly up-regulated 23-wk-old (laying phase) chicken ovaries compared with 6-wk-old (prepubertal phase). In reproductively active ovary, MMP1 expression (both mRNA protein) remained low prehierarchical preovulatory follicles...
Abstract The ability to generate germ cells from pluripotent stem (PSCs) is valuable for human regenerative medicine and animal breeding. Germ cell-like (GCLCs) have been differentiated mouse PSCs, but not porcine which are considered an ideal model cell applications. Here, we developed a defined culture system the induction of primordial (PGCLCs) induced PSCs (piPSCs). identity PGCLCs was characterized by observing morphology, detecting marker gene expression evaluating epigenetic...
The pluripotency of stem cells determines their developmental potential. While the states pluripotent are variable and interconvertible, mechanisms underlying acquisition maintenance remain largely elusive. Here, we identified that methylenetetrahydrofolate dehydrogenase (NAD+-dependent), methenyltetrahydrofolate cyclohydrolase (Mthfd2) plays an essential role in maintaining embryonic cell promoting complete reprogramming induced cells. Mechanistically, mitochondria, Mthfd2 maintains...
Abstract Background Fatty liver is a high incidence of perinatal disease in dairy cows caused by negative energy balance, which seriously threatens the postpartum health and milk production. It has been reported that lysine acetylation plays an important role substance metabolism. Predictably, most metabolic processes liver, as vital organ, are subjected to acetylation. Comparative acetylome study were used quantify hepatic tissues from severe fatty group normal group. Combined with...
After zygotic genome activation and lineage specification, zygotes develop into late blastocysts comprising three distinct cell types. The molecular mechanisms underlying this progress are largely unknown in pigs. Here, we intended to analyze an extensive set of regulators at the single-cell level define events involved development porcine blastocysts. Using a quantitative microfluidics approach single cells, detected mRNA levels 96 genes known function early embryonic maintenance stem...
Abundantly expressed factors in the oocyte cytoplasm can remarkably reprogram terminally differentiated germ cells or somatic into totipotent state within a short time. However, mechanism of different underlying reprogramming process remains uncertain.On basis Yamanaka OSKM induction method, MEF were induced and reprogrammed iPSCs under conditions oocyte-derived factor Wdr82 overexpression and/or knockdown, so as to assess efficiency. Meanwhile, cellular metabolism was monitored evaluated...
Abstract Induced pluripotent stem cells (iPSCs) have variable expression levels of a series genes that affect their potential, but the regulatory mechanisms controlling reprogramming remain unclear. By testing efficiency iPSC generation using Oct4, Sox2, Klf4 (termed OSK) plus one additional gene, we found Rab32 improved efficiency. We established system for detecting number and size lipid droplets autophagosomes per cell tracking morphological changes during reprogramming. Our results...
The simulation of the target capture process space manipulator is great significance for and task verification. By integrating physical numerical simulation, hardware-in-the-loop (HIL) very suitable to simulate complicated contact dynamics multibody in three-dimensional space. However, time delays system loop lead HIL error even instability. In this article, a distributed delay compensation approach proposed hybrid capture. force delay, calculation actuation mechanism are compensated situ....
Abstract Long non-coding RNAs (lncRNA) play a key role in the orchestration of transcriptional regulation during development and many other cellular processes. The importance regulatory co-expression network was highlighted identification mechanism these processes humans mice. However, elucidation properties porcine lncRNAs involved pre-implantation embryonic fibroblast reprogramming to induced pluripotent stem cell (iPSC) has been limited date. Using weighted gene analysis, we constructed...
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Insulin-like growth factor binding protein-2 (IGFBP-2), a protein of insulin-like (IGF) system, regulates the activity IGFs and also influences cellular function with endogenous activity. In mammals, IGFBP-2 is reported to affect ovarian follicle development steroidogenesis; however, its role in chicken ovary unknown. this study, we investigated mRNA expression novel transcript effect reproductive hormones 1 (IGF1) on granulosa cells follicles. The was significantly increased after selection...
It is not fully clear why there a higher contribution of pluripotent stem cells (PSCs) to the chimera produced by injection PSCs into 4-cell or 8-cell stage embryos compared with blastocyst injection. Here, we show that only embryonic (ESCs) but also induced (iPSCs) can generate F0 nearly 100% donor cell-derived mice embryo injection, and approach has "dose effect". Through an analysis PSC-secreted proteins, Activin A was found impede epiblast (EPI) lineage development while promoting...
Background and aims: Endoscopic submucosal dissection (ESD) is a pivotal technique for excision of early-stage esophageal tumors. However, its primary complication, postoperative stricture, significant challenge owing to the absence effective preventive measures. Adipose-derived stem cells (ADSCs) have emerged as promising treatment modality address this concern. In study, we aimed investigate, first time, efficacy allogenic ADSC injections in preventing stenosis after ESD. Methods: We...
Abstract Critical reprogramming factors resided predominantly in the oocyte or male pronucleus can enhance efficiency quality of induced pluripotent stem cells (iPSCs) induction. However, few exist had been verified. Here, we demonstrated that granulin ( Grn ), a factor enriched specifically pronucleus, significantly improve generation iPSCs from mouse fibroblasts. is highly expressed on Day 1, 3, 14 by four Yamanaka and functions at initial stage reprogramming. Transcriptome analysis...
The chaperonin containing TCP-1 complex protein 1 subunit zeta (CCT6A) is the only cytosolic in eukaryotes assisting folding of cytoplasmic proteins. Previous study revealed that mRNA expression chicken CCT6A gene was remarkably elevated sexually mature ovaries. However, mechanism underlying changes remains largely unknown. In this study, haplotypes caused by two single nucleotide polymorphisms (SNPs) promoter (g.-2215 T>C and g.-1959 T>C) were identified their associations with egg...