A5029898737- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Genetics, Aging, and Longevity in Model Organisms
- Renal and related cancers
- Plant tissue culture and regeneration
Shandong Agricultural University
2022-2024
Abundantly expressed factors in the oocyte cytoplasm can remarkably reprogram terminally differentiated germ cells or somatic into totipotent state within a short time. However, mechanism of different underlying reprogramming process remains uncertain.On basis Yamanaka OSKM induction method, MEF were induced and reprogrammed iPSCs under conditions oocyte-derived factor Wdr82 overexpression and/or knockdown, so as to assess efficiency. Meanwhile, cellular metabolism was monitored evaluated...
Abstract Critical reprogramming factors resided predominantly in the oocyte or male pronucleus can enhance efficiency quality of induced pluripotent stem cells (iPSCs) induction. However, few exist had been verified. Here, we demonstrated that granulin ( Grn ), a factor enriched specifically pronucleus, significantly improve generation iPSCs from mouse fibroblasts. is highly expressed on Day 1, 3, 14 by four Yamanaka and functions at initial stage reprogramming. Transcriptome analysis...