A5100782047- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Prostate Cancer Treatment and Research
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Signaling Pathways in Disease
- Cancer-related Molecular Pathways
- RNA regulation and disease
- Soil Carbon and Nitrogen Dynamics
- Environmental and Agricultural Sciences
- Cancer-related gene regulation
- RNA modifications and cancer
- Ophthalmology and Eye Disorders
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Hippo pathway signaling and YAP/TAZ
- Thyroid Cancer Diagnosis and Treatment
- Sepsis Diagnosis and Treatment
- Glaucoma and retinal disorders
- Nanopore and Nanochannel Transport Studies
- Kruppel-like factors research
- Crop Yield and Soil Fertility
- COVID-19 Clinical Research Studies
- Microtubule and mitosis dynamics
National Center for Nanoscience and Technology
2024-2025
Fudan University Shanghai Cancer Center
2022-2025
Shanghai Medical College of Fudan University
2022-2025
Zhejiang University
2021-2025
Ministry of Education of the People's Republic of China
2025
Zhejiang Cancer Hospital
2025
Xiamen University
2017-2024
Sichuan University
2011-2024
Umeå University
2021-2024
Shijiazhuang Academy of Agriculture and Forestry Sciences
2022-2024
The response of AMP-activated protein kinase (AMPK) to oxidative stress has been recently reported but the downstream signals this are largely unknown. Meanwhile, upstream events for activation nuclear factor erythroid-2-related factor-2 (Nrf2), a critical transcriptional activator antioxidative responses, remain unclear. In present study, we investigated relationship between AMPK and Nrf2 signal pathways in lipopolysaccharide (LPS)-triggered inflammatory system, which berberine (BBR), known...
Abstract Reactive oxygen species (ROS) production in phagocytes is a major defense mechanism against pathogens. However, the cellular self-protective such potential damage from oxidative stress remains unclear. Here we show that kinases Mst1 and Mst2 (Mst1/2) sense ROS maintain redox balance by modulating stability of antioxidant transcription factor Nrf2. Site-specific release recruits Mst1/2 cytosol to phagosomal or mitochondrial membrane, with subsequently activating phosphorylate kelch...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic poses an unprecedented public health crisis. Evidence suggests that SARS-CoV-2 infection causes dysregulation of the immune system. However, unique signature early responses remains elusive. We characterized transcriptome rhesus macaques and mice infected with SARS-CoV-2. Alarmin S100A8 was robustly induced in SARS-CoV-2-infected animal models as well COVID-19 patients. Paquinimod, a specific inhibitor S100A8/A9, could...
TLR4 signaling plays key roles in the innate immune response to microbial infection. Innate cells encounter different mechanical cues both health and disease adapt their behaviors. However, impact of sensing signals on signal-mediated remains unclear. Here we show that signalling augments macrophage bactericidal activity through sensor Piezo1. Bacterial infection or LPS stimulation triggers assembly complex Piezo1 remodel F-actin organization augment phagocytosis, mitochondrion-phagosomal...
The CRISPR-Cas13 system is an RNA-guided RNA-targeting and has been widely used in transcriptome engineering with potentially important clinical applications. However, it still controversial whether Cas13 exhibits collateral activity mammalian cells.
Emerging evidence has shown that open reading frames inside long noncoding RNAs (lncRNAs) could encode micropeptides. However, their roles in cellular energy metabolism and tumor progression remain largely unknown. Here, we identified a 94 amino acid–length micropeptide encoded by lncRNA LINC00467 colorectal cancer. We also characterized its conservation across higher mammals, localization to mitochondria, the concerted local functions. This peptide enhanced ATP synthase construction...
Abstract Proper follicle development is very important for the production of mature oocytes, which essential maintenance female fertility. This complex biological process requires precise gene regulation. The most abundant modification mRNA, N 6 -methyladenosine (m A), involved in many RNA metabolism processes, including splicing, translation, stability, and degradation. Here, we report that m A plays roles during oocyte development. Oocyte-specific inactivation key methyltransferase Mettl3...
Abstract Circulating extracellular vesicles (EVs) have emerged as a valuable source of cancer biomarkers. However, the high degree EV heterogeneity and complexity clinical samples pose challenge in sensitive identification tumor‐derived EVs. Here we introduce one‐step thermophoretic AND gate operation (Tango) assay that integrates polyethylene glycol (PEG)‐enhanced accumulation EVs simultaneous on membranes by dual‐aptamers recognition. By using Tango to detect with co‐presence EpCAM PSMA...
Abstract The systematic identification and functional characterization of noncanonical translation products, such as novel peptides, will facilitate the understanding human genome provide new insights into cell biology. Here, we constructed a high-coverage peptide sequencing reference library with 11,668,944 open reading frames employed an ultrafiltration tandem mass spectrometry assay to identify peptides. Through these methods, discovered 8945 previously unannotated peptides from normal...
AIMTo investigate the role of nuclear division cycle (NDC)80 in human hepatocellular carcinogenesis. METHODSNDC80 gene expression was analyzed by real-time reverse transcription polymerase chain reaction 47 paired carcinoma (HCC) and adjacent tissues.The HCC cell line SMMC-7721 transfected with lentivirus to silence endogenous NDC80 expression, which confirmed western blotting.The effects silencing on proliferation were evaluated Cellomics ArrayScan VTI imaging.Cell analysis apoptosis...
c-Myc, a well-established oncogene, plays an important role in the initiation and progression of various cancers, including prostate cancer. However, its mechanism cancer cell remains largely unknown whether there exist deubiquitinase targeting c-Myc also elusive.Bioinformatic analysis shRNA screening methods were used to identify potential deubiquitinases that correlate with gene signature. Cell proliferation viability measured by Cell-Counting-Kit 8 colony formation assays. A mouse...
Although conservation tillage has been widely implemented to address the challenge improve crop yield and soil quality with fewer environmental costs, its long-term effects on yields stoichiometry balance remain uncertain. Here, four different (17-year) practices (conventional (CT), deep scarification (DS), no (NT), ridge (RT)) were conducted in northern China evaluate their yield, nutrients, C sequestration, stoichiometry. The (DS, NT, RT) increased recent 5-year average by 12.2 %-20.1 %...
Abstract The ability to generate germ cells from pluripotent stem (PSCs) is valuable for human regenerative medicine and animal breeding. Germ cell-like (GCLCs) have been differentiated mouse PSCs, but not porcine which are considered an ideal model cell applications. Here, we developed a defined culture system the induction of primordial (PGCLCs) induced PSCs (piPSCs). identity PGCLCs was characterized by observing morphology, detecting marker gene expression evaluating epigenetic...
Abstract Chemo‐immunotherapy, combining systemic chemotherapeutic drugs and immune checkpoint blockers, is a promising paradigm in cancer treatment. However, challenges such as limited induction of responses toxicity have hindered its clinical applications. Here, zeolite imidazolate framework‐8 (ZIF‐8) that encapsulates mitoxantrone (MIT), an cell death (ICD)‐inducing agent (MIT@ZIF‐8), synthesized using one‐pot aqueous‐phase process. ZIF‐8 serves dual‐functional nanomaterial for...
D-Galactose (D-gal) can induce oxidative stress in non-cancer cells and result cell damage by disturbing glucose metabolism. However, the effect of D-gal on cancer is yet to be explored. In this study, we investigated toxicity malignant specifically neuroblastoma cells. As results, high concentrations had significant cells, whereas same no; viability loss via treatment was prominent (Neuro2a, SH-SY5Y, PC-3, HepG2) comparing non-malignant (NIH3T3 LO2). Differing from apoptosis induced H2O2,...
We conducted a follow-up survey for 55 Japanese encephalitis (JE) cases 1–2 years after hospital discharge in Gansu province, China. Community-, education-, and gender-matched healthy individuals without history of neurologic disease were selected as the comparison group. All subjects received neurological examination, intelligence quotient (IQ) measurement, adaptive behavior Wechsler memory scale (WMS) assessment. found 43.6% JE had at least one nervous system sequelae compared with 3.6%...