A5100718218- RNA and protein synthesis mechanisms
- Ion Transport and Channel Regulation
- Lipid Membrane Structure and Behavior
- Membrane-based Ion Separation Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Plant Stress Responses and Tolerance
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Plant Molecular Biology Research
- Pancreatic function and diabetes
- biodegradable polymer synthesis and properties
- Monoclonal and Polyclonal Antibodies Research
- Nanopore and Nanochannel Transport Studies
- Amino Acid Enzymes and Metabolism
- Photoreceptor and optogenetics research
- Carbon dioxide utilization in catalysis
- Ion channel regulation and function
- Advanced Photocatalysis Techniques
- Bacteriophages and microbial interactions
- CO2 Reduction Techniques and Catalysts
- Transgenic Plants and Applications
- Machine Learning in Bioinformatics
- Biotin and Related Studies
Zhejiang University
2006-2025
Ministry of Education of the People's Republic of China
2025
Zhejiang Cancer Hospital
2025
Huazhong University of Science and Technology
2017-2024
Jiangsu Normal University
2018-2024
Southwest University
2024
Chengdu University
2023-2024
Ningxia Medical University
2024
Jimei University
2024
Shanghai University
2024
This experiment was conducted to evaluate the effects of dietary inclusion Bacillus licheniformis on laying performance, egg quality, antioxidant enzyme activities, and intestinal barrier function hens. Hy-Line Variety W-36 hens (n = 540; 28 wk age) were randomized into 6 groups, each group with replications 15). The control received basal diet formulated maize soybean meal. treatment groups same diets supplemented 0.01, 0.02, 0.03, 0.06, 0.09% powder (2 × 1010 cfu/g) for an 8-wk trial....
Abstract The systematic identification and functional characterization of noncanonical translation products, such as novel peptides, will facilitate the understanding human genome provide new insights into cell biology. Here, we constructed a high-coverage peptide sequencing reference library with 11,668,944 open reading frames employed an ultrafiltration tandem mass spectrometry assay to identify peptides. Through these methods, discovered 8945 previously unannotated peptides from normal...
Aquaporins are involved in CO2 transport from the leaf intercellular air space to chloroplast, which contributes assimilation. However, mechanism of by rice (Oryza sativa L.) aquaporins is unknown. Here, we investigated function aquaporin OsPIP1;2 diffusion-associated photosynthesis and phloem sucrose transport. Moreover, grain yield lines overexpressing was determined. localized plasma membrane relative expression approximately 5-fold higher leaves presence an elevated concentration....
The unmanned surface vessel (USV) is an emerging marine tool with its advantages of automation and intelligence in recent years; the good trajectory tracking performance important capability. This paper proposes a novel prescribed fixed-time fault-tolerant control scheme for USV model parameter uncertainties, unknown external disturbances, actuator faults, based on improved disturbances observer. Firstly, proposed observer can not only accurately quickly estimate compensate lumped...
Abstract Background This study examined the effect of nuclear factor erythroid 2-related 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway on chronic obstructive pulmonary disease (COPD) and potential molecular mechanism. Methods A COPD mouse model was established by cigarette smoke exposure administered with either ML385 or dimethyl fumarate (DMF). Airway resistance mice detected. IL-1β IL-6 levels in alveolar lavage fluid were enzyme-linked immunosorbent assay. Hematoxylin eosin staining...
Routine strategies for the cell-free production of membrane proteins in presence detergent micelles and their efficient co-translational solubilization have been developed. Alternatively, expression rationally designed lipid bilayers becomes interesting particular biochemical studies. The synthesized would be directed into a more native-like environment transporters, channels or other supplied artificial membranes could allow subsequent functional analysis without any exposure to detergents....
To assess the optimal dietary protein requirement of adult red swamp crayfish (Procambarus clarkii) farming in rice field, six isoenergetic and isolipid diets were formulated to contain graded levels 28%, 31%, 34%, 37%, 40% 43% crude (called Diet 28–43 group). Each diet was assigned a triplicate 20 experimental with average initial body weight (15.17 ± 0.23) g for 42 days. Our results showed that final weight, gain rate specific growth 34, 37, 40 43 groups significantly higher than those...
Abstract Background: To investigate the effect of short-term cast immobilization on prognosis ankle fractures. Methods: A total 60 patients who underwent fracture surgery in our hospital from September 2021 to 2022 were included and divided into group (n=30) control (n=30). Both groups given open reduction internal fixation. After operation, used plaster bandage fix joint affected side functional position for 2 weeks; did not receive was only bandaged with routine wound dressings. The visual...
Black lipid bilayers, a general model system for biomembranes were studied diffusion rates of small molecules such as water or CO2 using advanced analysis techniques and cell free synthesized proteins. We provide evidence that by simple insertion proteins sterols the those decrease. Insertion permeable aquaporins restored well CO2-facilitating diffusion. impermeable decreased respective rates. Therefore, normal high cellular specific are mandatory.
Abstract High‐fat diet (HFD)‐induced obesity is a crucial risk factor for metabolic syndrome, mainly due to adipose tissue dysfunctions associated with it. However, the underlying mechanism remains unclear. This study has used genetic screening identify an obesity‐associated human lncRNA LINK‐A as critical molecule bridging microenvironment and energy expenditure in vivo by establishing HFD‐induced knock‐in (KI) mouse model. Mechanistically, HFD KI mice induce infiltration of inflammatory...
The continuous progress in the structural and functional characterization of aquaporins increasingly attracts attention to study their roles certain mammalian diseases. Although several structures have already been solved by crystallization, challenge producing sufficient amounts proteins still remains. CF (cell free) expression has emerged recent times as a promising alternative option order synthesize large quantities membrane proteins, focus this report was evaluate potential technique...
An approach for designing individual expression environments that reduce or prevent protein aggregation and precipitation is described. Inefficient folding of difficult proteins in unfavorable translation can cause significant losses overexpressed as precipitates inclusion bodies. A number chemical chaperones including alcohols, polyols, polyions polymers are known to have positive effects on stability. However, conventional approaches use such stabilizing agents only post-translationally...