A5043326130- Cancer-related molecular mechanisms research
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- RNA modifications and cancer
- RNA Research and Splicing
- Receptor Mechanisms and Signaling
- Machine Learning in Bioinformatics
- RNA and protein synthesis mechanisms
- Hippo pathway signaling and YAP/TAZ
- Advanced Fluorescence Microscopy Techniques
- Lipid metabolism and biosynthesis
- Cancer, Lipids, and Metabolism
- Gene Regulatory Network Analysis
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Cancer Immunotherapy and Biomarkers
- Ubiquitin and proteasome pathways
- Single-cell and spatial transcriptomics
- ATP Synthase and ATPases Research
- Signaling Pathways in Disease
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Protein Kinase Regulation and GTPase Signaling
- Calpain Protease Function and Regulation
- Phosphodiesterase function and regulation
Zhejiang University
2017-2025
Ministry of Education of the People's Republic of China
2025
Zhejiang Cancer Hospital
2022-2025
Southwestern Medical Center
2024
The University of Texas Southwestern Medical Center
2024
Johns Hopkins Medicine
2012-2021
Johns Hopkins University
2012-2021
University of Baltimore
2015
State Key Laboratory of Turbulence and Complex Systems
2008
Peking University
2008
Emerging evidence has shown that open reading frames inside long noncoding RNAs (lncRNAs) could encode micropeptides. However, their roles in cellular energy metabolism and tumor progression remain largely unknown. Here, we identified a 94 amino acid–length micropeptide encoded by lncRNA LINC00467 colorectal cancer. We also characterized its conservation across higher mammals, localization to mitochondria, the concerted local functions. This peptide enhanced ATP synthase construction...
Abstract Immune checkpoint blockade therapies targeting the PD-L1/PD-1 axis have demonstrated clear clinical benefits. Improved understanding of underlying regulatory mechanisms might contribute new insights into immunotherapy. Here, we identify transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) as a modulator PD-L1 post-translational modifications in tumor cells. Mechanistically, TMUB1 competes with HECT, UBA WWE (HUWE1), E3 ubiquitin ligase, to interact inhibit its...
Notch has been implicated in human cancers and is a putative therapeutic target. However, the regulation of activation nucleus remains largely uncharacterized. Therefore, characterizing detailed mechanisms governing degradation will identify attractive strategies for treating Notch-activated cancers. Here, we report that long noncoding RNA (lncRNA) BREA2 drives breast cancer metastasis by stabilizing Notch1 intracellular domain (NICD1). Moreover, reveal WW containing E3 ubiquitin protein...
Abstract Iron metabolism dysregulation is tightly associated with cancer development. But the underlying mechanisms remain poorly understood. Increasing evidence has shown that long noncoding RNAs (lncRNAs) participate in various metabolic processes via integrating signaling pathway. In this study, we revealed one iron-triggered lncRNA, target of YAP, LncRIM (LncRNA Related to Metabolism, also named ZBED5-AS1 and Loc729013 ), which effectively links Hippo pathway iron largely independent on...
Abstract The systematic identification and functional characterization of noncanonical translation products, such as novel peptides, will facilitate the understanding human genome provide new insights into cell biology. Here, we constructed a high-coverage peptide sequencing reference library with 11,668,944 open reading frames employed an ultrafiltration tandem mass spectrometry assay to identify peptides. Through these methods, discovered 8945 previously unannotated peptides from normal...
Hypertrophic cardiomyopathy (HCM) is the most prominent cause of sudden cardiac death in young individuals. Due to heterogeneity clinical manifestations, conventional HCM drugs have limitations for mitochondrial hypertrophic cardiomyopathy. Discovering more effective compounds would be substantial benefit further elucidating pathogenic mechanisms and treating patients with this condition. We previously reported MT-RNR2 variant associated that results dysfunction. Here, we screened a...
Abstract The regulation of L-type Ca 2+ channels by protein kinase A (PKA) represents a crucial element within cardiac, skeletal muscle and neurological systems. Although much work has been done to understand this in cardiac V 1.2 channels, relatively little is known about the closely related 1.4 which feature prominently visual system. Here we find that are indeed modulated PKA phosphorylation inhibitor -dependent inactivation (ICDI) motif. Phosphorylation region promotes occupancy...
Biology has been revolutionized by tools that allow the detection and characterization of protein-protein interactions (PPIs). Förster resonance energy transfer (FRET)-based methods have become particularly attractive as they quantitative studies PPIs within convenient relevant context living cells. We describe here an approach allows rapid construction live-cell FRET-based binding curves using a commercially available flow cytometer. illustrate simple method for absolutely calibrating...
Novel fluorescence resonance energy transfer-based genetically encoded reporters of calcineurin are constructed by fusing the two subunits with P2A-based linkers retaining expected native conformation calcineurin. Calcineurin display robust responses to calcium transients in HEK293 cells. The sensor correlated NFATc1 translocation dynamics sensors uniformly distributed neonatal myocytes and respond efficiently single electrically evoked show cumulative activation at frequencies 0.5 1 Hz. In...
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ABSTRACT Lipids are essential for tumours because of their structural, energetic, and signaling roles. While many cancer cells upregulate lipid synthesis, growing evidence suggests that simultaneously intensify the uptake circulating lipids carried by lipoproteins. Which mechanisms promote extracellular lipids, how this pool contributes to progression, poorly understood. Here, using functional genetic screens, we find lipoprotein confers resistance peroxidation ferroptotic cell death....
David T. Yue was a renowned biophysicist who dedicated his life to the study of Ca2+ signaling in cells. In wake passing, we are left not only with feeling great loss, but tremendous and impactful body work contributed by remarkable man. David's research spanned spectrum from atomic structure organ systems, quantitative rigor aimed at understanding fundamental mechanisms underlying biological function. Along way he developed new tools approaches, enabling own that contemporaries those will...