A5019902631- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Head and Neck Anomalies
- Hearing, Cochlea, Tinnitus, Genetics
- Teratomas and Epidermoid Cysts
- Tracheal and airway disorders
- Head and Neck Cancer Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Shoulder Injury and Treatment
- Shoulder and Clavicle Injuries
- Salivary Gland Tumors Diagnosis and Treatment
- Otolaryngology and Infectious Diseases
- Head and Neck Surgical Oncology
- Gut microbiota and health
- Viral-associated cancers and disorders
- Airway Management and Intubation Techniques
- Hearing Loss and Rehabilitation
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Pluripotent Stem Cells Research
- Foreign Body Medical Cases
- RNA Research and Splicing
Children's Hospital of Zhejiang University
2017-2025
University of South China
2024-2025
Peking Union Medical College Hospital
2009-2024
Nanjing University
2003-2024
Guangxi Medical University
2023-2024
Nanjing Medical University
2021-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine
2024
Zhejiang University
2008-2024
Nanjing Drum Tower Hospital
2022
MicroRNAs (miRNAs) are naturally occurring, small non-coding RNAs that mediate posttranscriptional regulation. Based on the level of sequence complementarity, miRNAs lead to degradation target mRNAs or suppression mRNA translation, thereby inhibiting synthesis proteins and achieving regulation genes. miRNAs, which exhibit tissue- temporal- specific expression, important negative regulatory decrease levels other functional play a crucial role in disease progression prognosis thus potential...
Hypertrophic cardiomyopathy (HCM) is the most common cause of sudden cardiac death in young individuals. A potential role mtDNA mutations HCM known. However, underlying molecular mechanisms linking to remain poorly understood due lack cell and animal models. Here, we generated induced pluripotent stem cell-derived cardiomyocytes (HCM-iPSC-CMs) from human patients a maternally inherited family who carry m.2336T>C mutation mitochondrial 16S rRNA gene (MT-RNR2). The results showed that resulted...
// Yang Ning 1, * , Fu Yong Zhang Haibin 1 Sima Hui Zhu Nan Guangshun Hepatobiliary Surgery Department V, Eastern Hospital, Second Military Medical University, Shanghai, China These authors have contributed equally to this work Correspondence to: Guangshun, e-mail: yangguangshunlab@sina.com Keywords: lincRNA-p21, hepatocellular carcinoma, ER stress, sorafenib Received: April 18, 2015 Accepted: July 08, Published: 20, 2015 ABSTRACT LincRNA-p21 is a downstream...
Abstract Peripheral nerve injuries are one of the most common types traumatic damage to nervous system. Treatment peripheral aims promote axon regrowth by imitating and improving microenvironment for sciatic regeneration. In this study, regeneration efficiency behavior nerves compared under three treatment strategies: 1) transplantation Schwann cell progenitors induced from purified neural crest stem cells; 2) implantation a multiscale scaffold based on high‐resolution 3D printing; 3) bionic...
Inner ear hair cells as mechanoreceptors are extremely important for hearing. Defects in a major cause of deafness. Induced pluripotent stem (iPSCs) promising regenerating inner and treating hearing loss. Here, we investigated migration, differentiation, synaptic connections transplanted otic epithelial progenitors (OEPs) derived from human iPSCs mouse cochlea. Human urinary isolated healthy donor were reprogramed to form that induced differentiate into OEPs cell-like cells....
Abstract Orthodontic force-induced osteogenic differentiation and bone formation at tension side play a pivotal role in orthodontic tooth movement (OTM). Platelet-derived growth factor-BB (PDGF-BB) is clinically proven factor during regeneration process with unclear mechanisms. Fibroblasts periodontal ligament (PDL) are considered to be mechanosensitive under force. Thus, we established OTM model investigate the correlation between PDGF-BB fibroblasts side. We confirmed that tensile force...
Given the complex nature of ulcerative colitis, combination therapy targeting multiple pathogenic genes and pathways colitis may be required. Unfortunately, current therapeutic strategies are usually based on independent chemical compounds or monoclonal antibodies, full potential has not yet been realized for treatment colitis. Here, we develop a synthetic biology strategy that integrates naturally existing circulating system small extracellular vesicles with artificial genetic circuits to...
Hypertrophic cardiomyopathy (HCM) is the most prominent cause of sudden cardiac death in young individuals. Due to heterogeneity clinical manifestations, conventional HCM drugs have limitations for mitochondrial hypertrophic cardiomyopathy. Discovering more effective compounds would be substantial benefit further elucidating pathogenic mechanisms and treating patients with this condition. We previously reported MT-RNR2 variant associated that results dysfunction. Here, we screened a...
Arthroscopic acromioclavicular joint (ACJ) reconstruction is widely applied in ACJ dislocation. A 4.0-mm bone tunnel the most used size clavicle; drilling a coracoid process also commonly applied. However, large-diameter tunnels clavicle and may cause many complications. Therefore, we present pragmatic surgical technique of with 2.0-mm looping suture around process. Due to minimal trauma bone, risk fracture can be significantly reduced; repeated will not occur, difficulty time greatly...
Objective:To explore the diagnosis and minimal invasive treatment of neonatal pyriform sinus fistula. Methods:A retrospective analysis was conducted on clinical data newborns diagnosed with fistula in Children's Hospital, Zhejiang University School Medicine from January 2016 to December 2023, including diagnostic process methods. Results:There were 8 children, 2 males 6 females, 7 cases left side 1 case right side. Six revealed a lump fetal neck during prenatal examination, two found have...
Objective: To evaluate device survival and identify risk factors for failure in pediatric cochlear implant (CI) surgery to guide strategies minimizing rates improving outcomes. Methods: A retrospective analysis was conducted on patients who underwent CI at the Children’s Hospital, Zhejiang University School of Medicine, from September 2008 2023. Device assessed using Kaplan-Meier method while independent influencing were analyzed log-rank test Cox regression model. Results: Among 602...
Background Liposarcoma of the spermatic cord is an extremely rare urological malignancy, with fewer than 300 cases reported in literature worldwide, and it often difficult to distinguish from inguinal hernias epididymal cysts. Typically, presents as asymptomatic, slow-growing paratesticular mass. Case presentation The case described herein involves a 59-year-old man who presented our hospital painless mass left scrotum. Physical examination revealed fixed, firm Ultrasonography scrotum...
Abstract The role of microRNA-1 (miR-1) in ischemia/reperfusion (I/R)-induced injury is not well illustrated. present study aimed to investigate the expression and potential target miR-1 myocardium a rat model I/R. apoptosis cardiomyocytes ischemic increased on day 1, then attenuated 3 7 post-I/R. Heat shot protein 90 (Hsp90) aa1 mRNA was decreased post-I/R Hsp90aa1 level day1 post-I/R, but reversed MiR-1 downregulated repression cultured neonatal ventricular cells (NRVCs) led an increase...
Optimization of nanofiber surface properties can lead to enhanced tissue regeneration outcomes in the context bone engineering. Herein, we developed a facile strategy decorate elctrospun nanofibers using extracellular matrix (ECM) order improve their performance for Electrospun PLLA (PLLA NF) were seeded with MC3T3-E1 cells and allowed grow two weeks harvest layer ECM on surface. After decellularization, found that was successfully preserved while maintaining nanostructure electrospun...
Most intrahepatic cholangiocarcinoma (ICC) patients experienced tumor recurrences even after curative resection, but the optimal cut-off time point and specific risk factors for early late of ICC have not been clearly defined. The objective current study was to define radical hepatectomy.Included in this were 259 who underwent surgery at our hospital between January 2005 December 2009. Recurrences these followed-up prospectively. Piecewise regression model minimum P value approach used...
Abstract Defects in the posttranscriptional modifications of mitochondrial tRNAs have been linked to human diseases, but their pathophysiology remains elusive. In this report, we investigated molecular mechanism underlying a deafness-associated tRNAIle 4295A>G mutation affecting highly conserved adenosine at position 37, 3′ adjacent tRNA’s anticodon. Primer extension and methylation activity assays revealed that m.4295A>G introduced tRNA methyltransferase 5 (TRMT5)-catalyzed...
Keratoacanthomas (KAs) and cutaneous squamous cell carcinomas (cuSCCs) develop in 15–30% of patients with BRAFV600E metastatic melanoma treated BRAF inhibitors (BRAFi). These lesions resemble mouse skin tumors induced by the two‐stage DMBA/TPA carcinogenesis protocol; this protocol BRAFi accelerates tumor induction. Since prior studies demonstrated cyclooxygenase 2 (COX‐2) is necessary for induction, we hypothesized that COX‐2 inhibition might prevent BRAFi‐accelerated tumors. Celecoxib, a...