A5101630281- Mitochondrial Function and Pathology
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Nanoparticle-Based Drug Delivery
- Metabolism and Genetic Disorders
- Cancer, Hypoxia, and Metabolism
- Inflammasome and immune disorders
- interferon and immune responses
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Protein Tyrosine Phosphatases
- Nanoplatforms for cancer theranostics
- Cancer-related molecular mechanisms research
- Renal cell carcinoma treatment
- Ferroptosis and cancer prognosis
- Polymer Surface Interaction Studies
- Graphene and Nanomaterials Applications
- TGF-β signaling in diseases
- Cancer Cells and Metastasis
- Microfluidic and Bio-sensing Technologies
- Bacteriophages and microbial interactions
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Nanomaterials in Catalysis
- Trace Elements in Health
- Galectins and Cancer Biology
Soochow University
2024
Zhejiang University
2013-2022
Sir Run Run Shaw Hospital
2022
Nanjing Medical University
2020-2021
Changzhou No.2 People's Hospital
2020-2021
Shandong Provincial Hospital
2020
Children's Hospital of Zhejiang University
2019-2020
Shandong University
2020
Ohio University
2017
Guangzhou Medical University
2011-2016
In this report, we investigated the pathophysiology of a novel hypertension-associated mitochondrial tRNA(Ala) 5655A → G (m.5655A G) mutation. The destabilization highly conserved base pairing (A1-U72) at aminoacyl acceptor stem by an m.5655A mutation altered function. An in vitro processing analysis showed that reduced efficiency precursor 5' end cleavage catalyzed RNase P. By using cybrids constructed transferring mitochondria from lymphoblastoid cell lines derived Chinese family into DNA...
Abstract Previously, we demonstrated that CD28 and CTLA-4 signaling control Casitas-B-lineage lymphoma (Cbl)-b protein expression, which is critical for T cell activation tolerance induction. However, the molecular mechanism(s) of this regulation remains to be elucidated. In study, found Cbl-b fails undergo tyrosine phosphorylation upon CD3 stimulation because SHP-1 recruited dephosphorylates Cbl-b, whereas costimulation abrogates interaction. support finding, cells lacking display...
Abstract Defects in the posttranscriptional modifications of mitochondrial tRNAs have been linked to human diseases, but their pathophysiology remains elusive. In this report, we investigated molecular mechanism underlying a deafness-associated tRNAIle 4295A>G mutation affecting highly conserved adenosine at position 37, 3′ adjacent tRNA’s anticodon. Primer extension and methylation activity assays revealed that m.4295A>G introduced tRNA methyltransferase 5 (TRMT5)-catalyzed...
Leber's hereditary optic neuropathy (LHON) is a maternal inheritance of eye disease because the mitochondrial DNA (mtDNA) mutations. We previously discovered 3866T>C mutation within gene for ND1 subunit complex I as possibly amplifying progression patients bearing disease-causing 11778G>A ND4 I. However, whether and how exacerbates were unknown. In this report, we showed that four Chinese families both m.3866T>C m.11778G>A mutations exhibited higher penetrances LHON than 6 pedigrees carrying...
Tubular epithelial-mesenchymal transition (EMT) has been widely accepted as the underlying mechanisms of renal interstitial fibrosis (RIF). The production reactive oxygen species (ROS) plays a vital role in tubular EMT process. purpose this study was to investigate involved molecular TGF-beta-induced and identify potential glutathione S-transferase alpha 3 (GSTA3) iTRAQ screening performed protein alterations rats underwent unilateral-ureteral obstruction (UUO). Protein expression GSTA3...
Mitochondrial tRNA processing defects were associated with human diseases but their pathophysiology remains elusively. The hypertension-associated m.4401A>G mutation resided at a spacer between mitochondrial tRNAMet and tRNAGln genes. An in vitro experiment revealed that the caused 59% 69% decreases 5' end efficiency of precursors, catalyzed by RNase P, respectively. Using umbilical vein endothelial cells-derived cybrids, we demonstrated all 8 tRNAs ND6 increases longer uncleaved precursors...
Mutations in the mitochondrial tRNAs have been reported to be important cause of hearing loss. However, only a few cases identified thus far and prevalence tRNA mutations hearing-impaired patients remain unclear. Here we performed mutational analysis 22 genes large cohort 887 Han Chinese subjects with loss by Sanger sequencing. The systemic evaluation putative pathogenic variants was further carried out frequency controls (<1%), phylogenetic analysis, structural...
Mammalian mitochondrial tRNA (mt-tRNA) plays a central role in the synthesis of 13 subunits oxidative phosphorylation complex system (OXPHOS). However, many aspects context-dependent expression mt-tRNAs mammals remain unknown. To investigate tissue-specific effects mt-tRNAs, we performed comprehensive analysis across five mice tissues (brain, heart, liver, skeletal muscle, and kidney) using Northern blot analysis. Striking differences 22 were observed, some cases differing by as much tenfold...
Abstract In this report, we investigated the molecular mechanism underlying a deafness-associated m.5783C &gt; T mutation that affects canonical C50-G63 base-pairing of TΨC stem tRNACys and immediately adjacent to 5′ end light-strand origin mitochondrial DNA (mtDNA) replication (OriL). Two dimensional agarose gel electrophoresis revealed marked decreases in intermediates including ascending arm Y-fork arcs spanning OriL mutant cybrids bearing mutation. mtDNA alterations were further...
A novel versatile system combining chemotherapy and photothermal therapy for cancer cells using CuS NCs is reported. were synthesized by a solid–liquid reaction between Cu2O nanocubes thiourea. While the hollow nanocage can be used to load anticancer drugs chemotherapy, convert NIR light into heat therapy. More importantly, display exceptionally high drug loading capacity of DOX with 500 wt% release pH-controllable, which spontaneous process under intracellular conditions. Compared or alone,...
Controllable biomineralization modification of cisplatin can alter the drug biodistribution with extended circulation time in blood. These changes increase passive tumor target and decrease non-specific accumulation significantly, which improve chemotherapeutic effect minimum side effects. As a service to our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed may be re-organized for online delivery, but not copy-edited or...
Abstract In this report, we investigated the molecular mechanism underlying a deafness-associated m.7516delA mutation affecting 5′ end processing sites of mitochondrial tRNAAsp and tRNASer(UCN). An in vitro experiment demonstrated that caused aberrant tRNASer(UCN) precursors, catalyzed by RNase P. Using cytoplasmic hybrids (cybrids) derived from one hearing-impaired Chinese family bearing control, asymmetrical effects on tRNAs heavy (H)-strand light (L)-strand polycistronic transcripts....
One-pot solution mineralization can encapsulate epirubicin (EPI) and pooled siRNAs (Pgp Bcl-2 siRNAs) in calcium phosphate (CaP). The resulting EPI-RNA-CaP nanocomplexes achieve a spatial–temporal synchronous effect to full-scale overcome sophisticated multiple drug resistance (MDR) by simultaneous inhibitions of efflux intracellular anti-apoptotic defense maximize the therapeutic efficacy. As service our authors readers, this journal provides supporting information supplied authors. Such...
Hematogenous metastatic spread causes most cancer patient deaths. Because circulating tumor cells (CTCs) are highly relevant to early spread, the capture or detection of these provides a diagnostic tool for patients with conditions. Herein, we demonstrate programmable electroactive multilayered material platform smart electrically induced "switch" that captures CTCs from biological plasma high efficiency and releases captured flexibly. The released still viable proliferative, which...
Mutations of mitochondrial transfer RNAs (mt-tRNAs) play a major role in wide range diseases because the vital these molecules translation.It has previously been reported that overexpression aminoacyl tRNA synthetases is effective at partially suppressing defects resulting from mutations their cognate mt-tRNAs cells.Here we report detailed analysis suppressive activities alanyl-tRNA synthetase (AARS2) on mt-tRNA Ala 5655 A>G mutant.Mitochondrial respiration, activity oxidative...
This study aimed to explore the regulative mechanisms of miR-27a-3p in chemo-resistance breast cancer cells.qRT-PCR was employed determine expression two cell lines, MCF-7 and MCF-7/adriamycin-resistant line (MCF-7/ADR). The lines were treated with mimics or inhibitors corresponding negative control (NC), respectively. changes investigated by qRT-PCR, CCK-8 assay, Western blot (WB), colony formation flow cytometry assay. Moreover, luciferase reporter assay analyzed verify downstream target...
Abstract The NLRP3 inflammasome is a multi-protein complex that triggers the activation of inflammatory caspase-1 and maturation IL-1β IL-18 in response to microbes danger signals cytosol host cells. However, how regulated not well defined. Here, we showed undergoes tyrosine phosphorylation macrophages upon stimulation with ATP, inflammsome stimulus, correlates its ubiquitination. Pretreating specific Src kinase inhibitor inhibits ubiquitination, which leads enhanced production IL-1β....
<title>Abstract</title> Background Disulfidptosis is a form of cell death, where generation nicotinamide adenine dinucleotide phosphate (NADPH) through the pentose pathway (PPP) play an important role. The discovery disulfidptosis provides new in-sights into lung adenocarcinoma (LUAD) therapeutics. Research Design and Methods: regulators (DSRs) was used to identify subgroups. Meanwhile, WGCNA single-cell analysis were performed genes related PPP (DPRGs). To determine risk signature, clinical...
Abstract NLRP3 inflammasome has been shown to be involved in antiviral and antibacterial innate immunity, as well the response adjuvants critical for adaptive immunity. However, inappropriate activation of contributes deleterious inflammatory syndromes. Although recent studies show that undergoes ubiquitination, mechanisms negatively regulating PAMPs or endogenous DAMPs are largely unknown. In this study, we found Cbl-b selectively inhibits activation, leaving other key pathways. Further...
Abstract Background: Gemcitabine is considered a classical agent for the treatment of patients with pancreatic cancer. However, gemcitabine resistance common cause failure, leading to poor survival. Therapy overcome would benefit This study investigated impact aspirin (ASA) in biological function cancer cells and potential mechanism. Methods: The MTT assay, wound healing assay annexin V-FITC/PI test was used determine whether ASA could inhibit cells. Besides, expression Bcl-2, Bax,...
Abstract Casitas B cell lymphoma protein-b (Cbl-b) is a RING finger family E3 ubiquitin ligase. It has been well documented that Cbl-b plays crucial role in T activation and tolerance induction. Our recent study also indicates regulates proallergic development allergic airway inflammation. Therefore, the regulation of expression cells important for its biological functions. previous studies demonstrate CD28 costimulation potentiates sel-ubiquitination, CTLA-4-B7 interaction induces...