A5100323229- Protein Tyrosine Phosphatases
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Galectins and Cancer Biology
- RNA Research and Splicing
- PI3K/AKT/mTOR signaling in cancer
- Carbohydrate Chemistry and Synthesis
- Wnt/β-catenin signaling in development and cancer
- Metabolomics and Mass Spectrometry Studies
- Helicobacter pylori-related gastroenterology studies
- CRISPR and Genetic Engineering
- Hydrogels: synthesis, properties, applications
- Cell Image Analysis Techniques
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Bioinformatics and Genomic Networks
- Microbial Natural Products and Biosynthesis
- Lipid metabolism and biosynthesis
- Genomics and Chromatin Dynamics
- Seaweed-derived Bioactive Compounds
- Polyamine Metabolism and Applications
- Fungal and yeast genetics research
- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
Center for Excellence in Brain Science and Intelligence Technology
2021-2025
University of Chinese Academy of Sciences
2014-2025
Chinese Academy of Sciences
2004-2024
Shanghai Institutes for Biological Sciences
2014-2024
Institute of Geochemistry
2024
Leiden University
2023-2024
Institute of Chemistry
2023
Chinese PLA General Hospital
2023
Beijing National Laboratory for Molecular Sciences
2023
Institute of Zoology
2022
Elucidating the cellular organization of cerebral cortex is critical for understanding brain structure and function. Using large-scale single-nucleus RNA sequencing spatial transcriptomic analysis 143 macaque cortical regions, we obtained a comprehensive atlas 264 transcriptome-defined cell types mapped their distribution across entire cortex. We characterized layer region preferences glutamatergic, GABAergic, non-neuronal types, as well regional differences in cell-type composition...
Pancreatic cancer is a highly malignant and lacks effective therapeutic targets. Protein-tyrosine phosphatase 1B (PTP1B), validated target for diabetes obesity, also plays critical positive or negative role in tumorigenesis. However, the of PTP1B pancreatic remains elusive. Here, we initially demonstrated that was expressed tumors, positively correlated with distant metastasis tumor staging, indicated poor survival. Then, inhibition either by shRNA specific small-molecule inhibitor...
The molecular and cellular organization of the primate cerebellum remains poorly characterized. We obtained single-cell spatial transcriptomic atlases macaque, marmoset, mouse cerebella identified primate-specific cell subtypes, including Purkinje cells molecular-layer interneurons, that show different expression glutamate ionotropic receptor Delta type subunit 2 ( GRID2 ) gene. Distinct gene profiles were found in anterior, posterior, vestibular regions all species, whereas region-selective...
Claustrum orchestrates brain functions via its connections with numerous regions, but molecular and cellular organization remains unresolved. Single-nucleus RNA sequencing of 227,750 macaque claustral cells identified 48 transcriptome-defined cell types, most glutamatergic neurons similar to deep-layer insular neurons. Comparison macaque, marmoset, mouse transcriptomes revealed macaque-specific types. Retrograde tracer injections at 67 cortical 7 subcortical regions defined four distinct...
Abstract Global profile of gene expression at single-cell resolution remains to be determined for primates. Using a recently developed technology (“Stereo-seq”), we have obtained comprehensive spatial transcriptome map the whole-brain level cynomolgus monkeys, with ∼600 genes per cell 10 μm-thick coronal sections (up 15 cm 2 in size). Large-scale single-nucleus RNA-seq analysis ∼1 million cells helped identify types corresponding Stereo-seq profiles, providing 3-D type atlas monkey brain....
Cartilage tissue engineering has attracted great attention in defect repair and regeneration. The utilization of bioactive scaffolds to effectively regulate the phenotype proliferation chondrocytes become an elemental means for cartilage On account simultaneous requirement mechanical biological performances tissue-engineered scaffolds, this work we prepared a naturally derived hydrogel composed kartogenin (KGN)-linked chitosan (CS-KGN) aldehyde-modified oxidized alginate (OSA) via highly...
Endothelial and fibroblast niches are crucial for epithelial organs. How these heterotypic cells interact is of great interest. In this study, we reveal an axis signaling in which fibroblasts relay Wnt signals from the endothelial niche to organize patterning. We generate Axin2-membrane GFP (mGFP) reporter mouse observe robust Wnt/β-catenin activities surrounding mammary epithelium. To enable cell-type-specific gene manipulation vitro, establish organoid system via coculture (ECs),...
MicroRNAs (miRNAs or miRs) have been shown to regulate hepatocellular carcinoma (HCC) metastasis. In the present study, we focused on functions of miR-1271 in HCC The downregulation was found be associated with venous infiltration, an advanced TNM stage (III+IV stage) and a shorter survival time. Our vitro vivo data demonstrated that prevented cell migration invasion, as well formation lung metastatic clusters. addition, markedly inhibit epithelial-mesenchymal transition (EMT) cells....
Abstract Summary: The studies of chromatin 3D structure help us to understand its formation and function. Techniques combining chromosome conformation capture next generation sequencing can information has been applied several different species cell lines. We built 3DGD (3D Genome Database), a database that currently collected Hi-C data on four species, for easy accessing visualization data. With the integration other omics such as genome-wide protein–DNA-binding data, this source would be...
Protein tyrosine phosphatase (PTP) 1B (PTP1B) plays a critical role in the regulation of obesity, Type 2 diabetes mellitus and other metabolic diseases. However, drug candidates exhibiting PTP1B selectivity oral bioavailability are currently lacking. Here, enzyme inhibitory characteristics pharmacological benefits 3-bromo-4,5-bis(2,3-dibromo-4,5-dihydroxybenzyl)-1,2-benzenediol (BDB) were investigated vitro vivo.Surface plasmon resonance (SPR) assay was performed to validate direct binding...
RNA-binding proteins (RBPs) lie at the center of posttranscriptional regulation and dysregulation RBPs contributes to diabetes. Therefore, modulation is anticipated become a potential therapeutic approach CYC27 synthetic derivative marine bromophenol BDB, which isolated from red alga Rhodomela confervoides. In this study, we found that significantly lowered blood glucose levels diabetic BKS db mice. Moreover, effectively ameliorated dyslipidemia in mice by reducing their total serum...
Chromatin interactions medicated by genomic elements located throughout the genome play important roles in gene regulation and can be identified with technologies such as high-throughput chromosome conformation capture (Hi-C), followed next-generation sequencing. These techniques were wildly used to reveal relative spatial disposition of chromatins human, mouse yeast. Unlike metazoan where CTCF plays major mediating chromatin interactions, yeast, transcription factors (TFs) involved this...
Protein tyrosine phosphatase 1B (PTP1B) is a widely confirmed target of the type 2 diabetes mellitus (T2DM) treatment. Herein, we reported highly specific PTP1B inhibitor 2,2',3,3'-tetrabromo-4,4',5,5'-tetrahydroxydiphenylmethane (compound 1), which showed promising hypoglycemic activity in diabetic BKS db mice. With IC