A5011835067- Hepatocellular Carcinoma Treatment and Prognosis
- Immune cells in cancer
- Circular RNAs in diseases
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Liver physiology and pathology
- Ferroptosis and cancer prognosis
- Cancer Cells and Metastasis
- Cancer Mechanisms and Therapy
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Liver Disease Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Hepatitis B Virus Studies
- Pancreatic and Hepatic Oncology Research
- Organ Transplantation Techniques and Outcomes
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Cancer-related gene regulation
- Hepatitis C virus research
Fudan University
2016-2025
Zhongshan Hospital
2016-2025
Capital Medical University
2020-2025
Ministry of Education of the People's Republic of China
2013-2025
Southern Medical University Shenzhen Hospital
2021-2024
Capital University
2024
Beijing Shijitan Hospital
2024
Sun Yat-sen University
2009-2024
The First Affiliated Hospital, Sun Yat-sen University
2009-2024
Chinese PLA General Hospital
2024
Neutrophils can either promote or inhibit tumor progression, depending on the microenvironment, via release of cytokines. Neither factors produced by tumor-associated neutrophils (TANs) nor their effects progression have been characterized. We investigated roles TANs in hepatocellular carcinoma (HCC) using cell lines and immune cells isolated from patients.We performed studies with HepG2, PLC/PRF/5, MHCC97H, HCCLM3 human Hepa1-6 H22 mouse HCC lines; expression chemokines cytokines were...
<b><i>Background:</i></b> Primary liver cancer, around 90% are hepatocellular carcinoma in China, is the fourth most common malignancy and second leading cause of tumor-related death, thereby posing a significant threat to life health Chinese people. <b><i>Summary:</i></b> Since publication <i>Guidelines for Diagnosis Treatment Liver Cancer (2017 Edition)</i> 2018, additional high-quality evidence has emerged with relevance...
More than 60% of patients with hepatocellular carcinoma (HCC) do not receive curative therapy as a result late clinical presentation and diagnosis. We aimed to identify plasma microRNAs for diagnosing hepatitis B virus (HBV) -related HCC.Plasma microRNA expression was investigated three independent cohorts including 934 participants (healthy, chronic B, cirrhosis, HBV-related HCC), recruited between August 2008 June 2010. First, we used microarray screen 723 in 137 samples HCC. Quantitative...
Abstract Liver metastasis, the leading cause of colorectal cancer mortality, exhibits a highly heterogeneous and suppressive immune microenvironment. Here, we sequenced 97 matched samples by using single-cell RNA sequencing spatial transcriptomics. Strikingly, metastatic microenvironment underwent remarkable reprogramming immunosuppressive cells such as MRC1+ CCL18+ M2-like macrophages. We further developed scMetabolism, computational pipeline for quantifying metabolism, observed that those...
CXCL5 (epithelial neutrophil-activating peptide-78) is a member of proangiogenic subgroup the CXC-type chemokine family small, secreted proteins. Recently, evidence that involved in carcinogenesis and cancer progression has emerged. To investigate role tumor growth, invasion, prognosis hepatocellular carcinoma (HCC), we examined messenger RNA (mRNA) protein levels HCC cell lines with various metastatic potentials three independent cohorts 919 patients. We found expression was increased...
Objective The lack of highly sensitive and specific diagnostic biomarkers is a major contributor to the poor outcomes patients with hepatocellular carcinoma (HCC). We sought develop non-invasive approach using circulating cell-free DNA (cfDNA) for early detection HCC. Design Applying 5hmC-Seal technique, we obtained genome-wide 5-hydroxymethylcytosines (5hmC) in cfDNA samples from 2554 Chinese subjects: 1204 HCC, 392 chronic hepatitis B virus infection (CHB) or liver cirrhosis (LC) 958...
MicroRNAs (miRNAs) play a critical role in regulation of tumor metastasis. However, the these molecules hepatocellular carcinoma (HCC) has not been fully elucidated. In this study, we employed miRNA‐sequencing and identified 22 miRNAs involved HCC One these, miR‐28‐5p, was down‐regulated HCCs. This down‐regulation correlated with metastasis, recurrence, poor survival. Biofunctional investigations revealed that miR‐28‐5p deficiency promoted growth metastasis nude mice without altering vitro...
There is growing evidence that single-stranded, circular RNA (circRNA) plays a key role in the development of certain cancers, including hepatocellular carcinoma (HCC). It less clear, however, what circRNA HCC metastasis.In this study, through sequencing, we identified circRNA: circASAP1 (a derived from exons 2 and 3 ASAP1 gene, hsa_circ_0085616), which associated with pulmonary metastasis after curative resection patients HCC. CircASAP1 was overexpressed cell lines high metastatic potential...
Tumor-associated neutrophils (TANs) play a crucial role in tumor development and progression the cancer microenvironment. Despite increased understanding of TAN contributions to hepatocellular carcinoma (HCC) prognosis, direct interaction between TANs HCC cells is not fully understood. In this study, we tested effect on vitro vivo investigated mechanism them. Our results showed that secreted bone morphogenetic protein 2 transforming growth factor beta triggered microRNA 301b-3p (miR-301-3p)...
Primary liver cancer, of which around 75-85% is hepatocellular carcinoma in China, the fourth most common malignancy and second leading cause tumor-related death, thereby posing a significant threat to life health Chinese people.Since publication Guidelines for Diagnosis Treatment Liver Cancer China June 2017, were updated by National Health Commission December 2019, additional high-quality evidence has emerged from researchers worldwide regarding diagnosis, staging, treatment that requires...
Abstract Background and Aims We previously demonstrated that cancer‐associated fibroblasts (CAFs) promote tumor growth through recruitment of myeloid‐derived suppressor cells (MDSCs). 5‐lipoxygenase (5‐LO) is highly expressed in myeloid critical for synthesizing leukotriene B4 (LTB4), which involved progression by activating its receptor type 2 (BLT2). In this study, we investigated whether how CAFs regulate MDSC function to enhance cancer stemness, the driving force aggressiveness...
Abstract Although circular RNAs (circRNA) are known to modulate tumor initiation and progression, their role in hepatocellular carcinoma (HCC) metastasis remains poorly understood. Here, three metastasis-associated circRNAs identified a previous circRNA-sequencing study were screened validated two HCC cohorts. CircRPN2 was downregulated highly metastatic cell lines tissues with metastasis. Patients lower circRPN2 levels displayed shorter overall survival higher rates of cumulative...
Abstract Advanced intrahepatic cholangiocarcinoma (ICC) has a dismal prognosis. Here, we report the efficacy and safety of combining toripalimab, lenvatinib, gemcitabine plus oxaliplatin (GEMOX) as first-line therapy for advanced ICC. Thirty patients with pathologically confirmed ICC received intravenous (1 g/m 2 ) on Days 1 8 (85 mg/m Q3W six cycles along toripalimab (240 mg) oral lenvatinib (8 once daily one year. The expression programmed death-ligand (PD-L1) genetic status was...
Abstract Background and Aims Macrophages are prominent components of solid tumors exhibit distinct functions in different tumor microenvironments. Exosomes emerging as necessary mediators the cross‐talk between cells microenvironment. However, underlying mechanisms exosomes involving into crosstalk macrophages during disease progression intrahepatic cholangiocarcinoma (ICC) have not been yet fully realized. Approach Results We found that ICC tissues up‐regulated expression levels...
Abstract Purpose: Novel therapeutic strategies are needed to prevent the tumor recurrence or metastasis after liver transplantation for hepatocellular carcinoma (HCC). This study was investigate effect of rapamycin, alone and in combination with sorafenib, on HCC vivo. Experimental Design: Xenograft a highly metastatic human (LCI-D20) used evaluate primary growth lung treatment rapamycin sorafenib. Tumor cell proliferation determined by Ki-67 immunostaining. To detect apoptosis, terminal...
Mitochondria lie at the heart of innate immunity, and aberrant mitochondrial activity contributes to immune activation chronic inflammatory diseases, including liver cancers. Mitophagy is a selective process for removing dysfunctional mitochondria. The link between mitophagy inflammation in tumorigenesis remains largely unexplored. We observed that FUN14 domain-containing 1 (FUNDC1), previously characterized receptor, accumulates most human hepatocellular carcinomas (HCCs), we thus explored...
Circulating tumor DNA (ctDNA) is increasingly recognized as liquid biopsy to profile genome. Droplet digital PCR (ddPCR) a highly sensitive and easily operable platform for mutant detection. Here, we tried detect ctDNA in hepatocellular carcinoma (HCC) patients using ddPCR.Studies sequencing the genome of HCCs COSMIC (Catalogue Somatic Mutations Cancer) database were reviewed identify hotspot mutations. cell-free DNAs (cfDNAs) extracted from 1 ml preoperative plasma sample analyzed...
The Wnt/β-catenin (β-cat) pathway plays a critical role in cancer. Using hydrocarbon-stapled peptide technologies, we aim to develop potent, selective inhibitors targeting this by disrupting the interaction of β-cat with its coactivators B-cell lymphoma 9 (BCL9) and 9-like (B9L). We identified set peptides, including hsBCL9CT-24, that robustly inhibits activity suppresses cancer cell growth. In animal models, these peptides exhibit potent anti-tumor effects, favorable pharmacokinetic...
Abstract Aim Circulating tumor cells (CTC) have shown promise in predicting the outcomes of adjuvant treatments for several malignancies. The clinical significance CTC efficacy tyrosine kinase inhibitor (TKI) administration patients with hepatocellular carcinoma (HCC) was unclear. Methods A total 429 who underwent liver transplantation HCC had provided 335 preoperative and 373 postoperative blood samples that could be used detection (pre‐CTC post‐CTC). association pre‐CTC post‐CTC findings...
CD133+ cancer stem cells (CSC) contribute to hepatocellular carcinoma (HCC) progression and resistance therapy. Bone morphogenetic protein BMP4 plays an important role in hepatogenesis hepatic cell differentiation, but little is known about its function CSCs. In this study, we showed that high-dose exogenous promotes HCC CSC differentiation inhibits the self-renewal, chemotherapeutic resistance, tumorigenic capacity of these cells. Interestingly, found low-dose upregulated CD133 expression...
The clinical significance of circulating cell-free DNA (cfDNA) integrity as diagnostic and surveillance biomarker in hepatocellular carcinoma (HCC) was investigated compared to that alpha fetoprotein (AFP).Liver cancer patients had lower cfDNA than those with benign diseases (P = 0.0167) healthy individuals 0.0025).Patients HCC non-HCC liver cancers 0.7356), 0.9138) comparable respectively.cfDNA increased after hepatectomy 0.0003).The AUCs for detecting by AFP were 0.705 0.005) 0.605 0.156),...
Early recurrence of hepatocellular carcinoma (HCC) after curative resection is common. However, the association between genetic mechanisms and early HCC recurrence, especially in Chinese patients, remains largely unknown.We performed whole-genome sequencing (49 cases), whole-exome (18 deep targeted (115 cases) on 182 primary samples. Focusing WNK2, we used Sanger qPCR to evaluate all coding exons copy numbers that gene an additional 554 We also explored functional effect mechanism WNK2 tumor...