A5100663149- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Pancreatic and Hepatic Oncology Research
- Cancer Mechanisms and Therapy
- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- Organ Transplantation Techniques and Outcomes
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- Hepatitis B Virus Studies
- Cancer, Lipids, and Metabolism
- Liver Diseases and Immunity
- Liver Disease Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Epigenetics and DNA Methylation
- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- interferon and immune responses
- Liver Disease and Transplantation
Fudan University
2016-2025
Zhongshan Hospital
2016-2025
Peking University People's Hospital
2025
Peking University
2025
Cancer Institute (WIA)
2009-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Ministry of Education of the People's Republic of China
2018-2025
China Tobacco Guangxi Industrial (China)
2025
Affiliated Hospital of Southwest Medical University
2025
Fudan University Shanghai Cancer Center
2005-2024
Abstract A newly identified coronavirus, 2019-nCoV, has been posing significant threats to public health since December 2019. ACE2, the host cell receptor for severe acute respiratory syndrome coronavirus (SARS), recently demonstrated in mediating 2019-nCoV infection. Interestingly, besides system, substantial proportion of SARS and patients showed signs various degrees liver damage, mechanism implication which have not yet determined. Here, we performed an unbiased evaluation type specific...
Abstract Purpose: The aberrant expression of programmed cell death 1 ligands and 2 (PD-Ls) on tumor cells dampens antitumor immunity, resulting in immune evasion. In this study, we investigated the PD-Ls human hepatocellular carcinoma (HCC) to define their prognostic significance after curative surgery. Experimental Design: Immunohistochemistry was used investigate as well granzyme B+ cytotoxic FoxP3+ regulatory T infiltration tissue microarrays containing 240 randomly selected HCC patients...
The Asia-Pacific Primary Liver Cancer Expert (APPLE) Consensus Statement on the treatment strategy for patients with intermediate-stage hepatocellular carcinoma (HCC) was established August 31, 2019, in Sapporo, Hokkaido during 10th Annual APPLE Meeting. This manuscript summarizes international consensus statements developed at 2019. Transarterial chemoembolization (TACE) is only guideline-recommended global standard of care HCC. However, not all benefit from TACE because HCC a heterogeneous...
Objective The lack of highly sensitive and specific diagnostic biomarkers is a major contributor to the poor outcomes patients with hepatocellular carcinoma (HCC). We sought develop non-invasive approach using circulating cell-free DNA (cfDNA) for early detection HCC. Design Applying 5hmC-Seal technique, we obtained genome-wide 5-hydroxymethylcytosines (5hmC) in cfDNA samples from 2554 Chinese subjects: 1204 HCC, 392 chronic hepatitis B virus infection (CHB) or liver cirrhosis (LC) 958...
Combined therapy with tyrosine kinase inhibitors (TKIs) and anti-PD-1 antibodies has shown high tumor response rates for patients unresectable hepatocellular carcinoma (HCC). However, using this treatment strategy to convert initially HCC resectable was not reported.Consecutive who received first-line combined TKI/anti-PD-1 were analyzed. Tumor resectability evaluated via imaging every 2 months (±2 weeks) RECIST v1.1. Resectability criteria (1) R0 resection could be achieved sufficient...
Little is known about the transcriptomic plasticity and adaptive mechanisms of circulating tumor cells (CTCs) during hematogeneous dissemination. Here we interrogate transcriptome 113 single CTCs from 4 different vascular sites, including hepatic vein (HV), peripheral artery (PA), (PV) portal (PoV) using single-cell full-length RNA sequencing in hepatocellular carcinoma (HCC) patients. We reveal that transcriptional dynamics were associated with stress response, cell cycle immune-evasion...
Hepatocellular carcinoma (HCC) is an immunotherapy-resistant malignancy characterized by high cellular heterogeneity. The diversity of cell types and the interplay between tumor non-tumor cells remain to be clarified. Single RNA sequencing human mouse HCC tumors revealed heterogeneity cancer-associated fibroblast (CAF). Cross-species analysis determined prominent CD36+ CAFs exhibited high-level lipid metabolism expression macrophage migration inhibitory factor (MIF). Lineage-tracing assays...
The overall response rate for anti-PD-1 therapy remains modest in hepatocellular carcinoma (HCC). We found that a combination of IFNα and anti-PD-1-based immunotherapy resulted enhanced antitumor activity patients with unresectable HCC. In both immunocompetent orthotopic spontaneous HCC models, synergized the treatment led to significant enrichment cytotoxic CD27+CD8+ T cells. Mechanistically, suppressed HIF1α signaling by inhibiting FosB transcription cells, resulting reduced glucose...
Abstract Although circular RNAs (circRNA) are known to modulate tumor initiation and progression, their role in hepatocellular carcinoma (HCC) metastasis remains poorly understood. Here, three metastasis-associated circRNAs identified a previous circRNA-sequencing study were screened validated two HCC cohorts. CircRPN2 was downregulated highly metastatic cell lines tissues with metastasis. Patients lower circRPN2 levels displayed shorter overall survival higher rates of cumulative...
Despite remarkable advances in treatment, most patients with hepatocellular carcinoma (HCC) respond poorly to anti-programmed cell death 1 (anti-PD1) therapy. A deeper insight into the tolerance mechanism of HCC against this therapy is urgently needed.We performed next-generation sequencing, multiplex immunofluorescence, and dual-color immunohistochemistry constructed an orthotopic xenograft tumor model identify key gene associated anti-PD1 tolerance. spontaneously tumorigenic transgenic...
Abstract Dissecting and understanding the cancer ecosystem, especially that around tumor margins, which have strong implications for cell infiltration invasion, are essential exploring mechanisms of metastasis developing effective new treatments. Using a novel border scanning digitization model enabled by nanoscale resolution-SpaTial Enhanced REsolution Omics-sequencing (Stereo-seq), we identified 500 µm-wide zone centered in patients with liver cancer, referred to as “the invasive zone”. We...
Abstract Intrahepatic cholangiocarcinoma (iCCA) is a highly heterogeneous cancer with limited understanding of its classification and tumor microenvironment. Here, by performing single-cell RNA sequencing on 144,878 cells from 14 pairs iCCA tumors non-tumor liver tissues, we find that S100P SPP1 are two markers for perihilar large duct type (iCCA phl ) peripheral small pps ). + SPP1− has significantly reduced levels infiltrating CD4 T cells, CD56 NK increased CCL18 macrophages PD1 CD8...
Abstract Advanced intrahepatic cholangiocarcinoma (ICC) has a dismal prognosis. Here, we report the efficacy and safety of combining toripalimab, lenvatinib, gemcitabine plus oxaliplatin (GEMOX) as first-line therapy for advanced ICC. Thirty patients with pathologically confirmed ICC received intravenous (1 g/m 2 ) on Days 1 8 (85 mg/m Q3W six cycles along toripalimab (240 mg) oral lenvatinib (8 once daily one year. The expression programmed death-ligand (PD-L1) genetic status was...
Abstract As the first rate-limiting enzyme in fatty acid oxidation (FAO), CPT1 plays a significant role metabolic adaptation cancer pathogenesis. FAO provides an alternative energy supply for cells and is required cell survival. Given high proliferation rate of cells, nucleotide synthesis gains prominence rapidly proliferating cells. In present study, we found that CPT1A determining factor abnormal activation nasopharyngeal carcinoma (NPC) highly expressed NPC biopsies. dramatically affects...
Abstract Background and Aims Macrophages are prominent components of solid tumors exhibit distinct functions in different tumor microenvironments. Exosomes emerging as necessary mediators the cross‐talk between cells microenvironment. However, underlying mechanisms exosomes involving into crosstalk macrophages during disease progression intrahepatic cholangiocarcinoma (ICC) have not been yet fully realized. Approach Results We found that ICC tissues up‐regulated expression levels...