A5101820502- Circular RNAs in diseases
- Cholangiocarcinoma and Gallbladder Cancer Studies
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Peptidase Inhibition and Analysis
- Cancer Mechanisms and Therapy
- Cancer Cells and Metastasis
- Liver Disease and Transplantation
- Cancer Immunotherapy and Biomarkers
- Hepatocellular Carcinoma Treatment and Prognosis
- Liver Disease Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Ubiquitin and proteasome pathways
- Cancer, Lipids, and Metabolism
- Viral-associated cancers and disorders
- RNA regulation and disease
- Chemokine receptors and signaling
- Organ Transplantation Techniques and Outcomes
- Caveolin-1 and cellular processes
Southwest Medical University
2023-2025
Affiliated Hospital of Southwest Medical University
2021-2025
Chongqing University
2024
Fudan University
2015-2024
Zhongshan Hospital
2015-2024
Sun Yat-sen University
2018-2024
The First Affiliated Hospital, Sun Yat-sen University
2018-2024
Xijing Hospital
2021-2023
Cancer Institute (WIA)
2016-2023
Air Force Medical University
2021-2023
Neutrophils can either promote or inhibit tumor progression, depending on the microenvironment, via release of cytokines. Neither factors produced by tumor-associated neutrophils (TANs) nor their effects progression have been characterized. We investigated roles TANs in hepatocellular carcinoma (HCC) using cell lines and immune cells isolated from patients.We performed studies with HepG2, PLC/PRF/5, MHCC97H, HCCLM3 human Hepa1-6 H22 mouse HCC lines; expression chemokines cytokines were...
CXCL5 (epithelial neutrophil-activating peptide-78) is a member of proangiogenic subgroup the CXC-type chemokine family small, secreted proteins. Recently, evidence that involved in carcinogenesis and cancer progression has emerged. To investigate role tumor growth, invasion, prognosis hepatocellular carcinoma (HCC), we examined messenger RNA (mRNA) protein levels HCC cell lines with various metastatic potentials three independent cohorts 919 patients. We found expression was increased...
Macrophages are a major component of the leukocyte infiltrate tumors and play pivotal role in progression hepatocellular carcinoma (HCC). However, molecular mechanisms by which macrophages promote HCC invasion poorly understood. The present study was undertaken to investigate relationship between epithelial-mesenchymal transition (EMT) HCC. Double-staining immunohistochemistry used observe association EMT markers clinical samples it showed that primarily occurred at edge tumor nest,...
MicroRNAs (miRNAs) play a critical role in regulation of tumor metastasis. However, the these molecules hepatocellular carcinoma (HCC) has not been fully elucidated. In this study, we employed miRNA‐sequencing and identified 22 miRNAs involved HCC One these, miR‐28‐5p, was down‐regulated HCCs. This down‐regulation correlated with metastasis, recurrence, poor survival. Biofunctional investigations revealed that miR‐28‐5p deficiency promoted growth metastasis nude mice without altering vitro...
There is growing evidence that single-stranded, circular RNA (circRNA) plays a key role in the development of certain cancers, including hepatocellular carcinoma (HCC). It less clear, however, what circRNA HCC metastasis.In this study, through sequencing, we identified circRNA: circASAP1 (a derived from exons 2 and 3 ASAP1 gene, hsa_circ_0085616), which associated with pulmonary metastasis after curative resection patients HCC. CircASAP1 was overexpressed cell lines high metastatic potential...
Tumor-associated neutrophils (TANs) play a crucial role in tumor development and progression the cancer microenvironment. Despite increased understanding of TAN contributions to hepatocellular carcinoma (HCC) prognosis, direct interaction between TANs HCC cells is not fully understood. In this study, we tested effect on vitro vivo investigated mechanism them. Our results showed that secreted bone morphogenetic protein 2 transforming growth factor beta triggered microRNA 301b-3p (miR-301-3p)...
Abstract Although circular RNAs (circRNA) are known to modulate tumor initiation and progression, their role in hepatocellular carcinoma (HCC) metastasis remains poorly understood. Here, three metastasis-associated circRNAs identified a previous circRNA-sequencing study were screened validated two HCC cohorts. CircRPN2 was downregulated highly metastatic cell lines tissues with metastasis. Patients lower circRPN2 levels displayed shorter overall survival higher rates of cumulative...
Abstract Background and Aims Macrophages are prominent components of solid tumors exhibit distinct functions in different tumor microenvironments. Exosomes emerging as necessary mediators the cross‐talk between cells microenvironment. However, underlying mechanisms exosomes involving into crosstalk macrophages during disease progression intrahepatic cholangiocarcinoma (ICC) have not been yet fully realized. Approach Results We found that ICC tissues up‐regulated expression levels...
CXCL5 is a member of the CXC-type chemokine family that may play role in carcinogenesis and cancer progression. This study investigates biological function clinical significance intrahepatic cholangiocarcinoma (ICC). We demonstrated was overexpressed ICC cell lines tumor samples compared with paired normal tissues. had direct chemoattractant effect on neutrophils vitro through PI3K-Akt extracellular signal-regulated kinase 1/2 signaling pathways. In animal studies, promoted growth metastasis...
Heterogeneous ribonucleoprotein (hnRNP) A1 is a member of the A/B subfamily ubiquitously expressed hnRNPs, which have wide variety functions in gene expression and signal transduction. To investigate biological function clinical significance hnRNP hepatocellular carcinoma (HCC), we measured four HCC cell lines two independent cohorts patients. We found that was overexpressed highly metastatic tumor tissues patients with recurrent HCC. Knockdown cells caused significant decrease invasion,...
Expression of heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) has been reported to be dysregulated in tumors, but its specific contributions tumor formation and progression are not fully understood. Here, we demonstrate that HNRNPAB is overexpressed highly metastatic cells tissues from patients with hepatocellular carcinoma (HCC) recurrence. We found overexpression promoted epithelial-mesenchymal transition (EMT) a manner associated HCC metastasis vitro vivo. RNA interference-mediated...
Natural killer (NK) cell can inhibit tumor initiation and regulates metastatic dissemination, acting as key mediators of the innate immune response. Intrinsic factors modulating NK cells infiltration its anticancer activity remain poorly characterized. We investigated roles dysregulation micro(mi)RNAs in progression hepatocellular carcinoma (HCC). Methods: Small RNA sequencing were used to detect miRNA profiles tissues from HCC patients with (n=14) or without (n=13) pulmonary metastasis...
Background Tumor-associated neutrophils (TANs) and macrophages (TAMs) can each influence cancer growth metastasis, but their combined effects in intrahepatic cholangiocarcinoma (ICC) remain unclear. Methods We explored the distributions of TANs TAMs patient-derived ICC samples by multiplex immunofluorescent staining tested separate on vitro vivo. then investigated mechanistic basis using PCR array, western blot analysis ELISA experiments. Finally, we validated our results a tissue microarray...
Elongator is a histone acetyl-transferase complex consisting of six subunits, and highly conserved in eukaryotic organisms. Here, we isolated two novel mutants, elp2 elp6, during genetic screening for ABA-hypersensitive Arabidopsis mutants. Map-based cloning identified ELP2 ELP6, which encode the orthologs yeast respectively. Another subunit mutant, elp4/elo1, was obtained from SALK T-DNA collection. The elp1/abo1/elo2 mutant previous study. All four mutants had narrow leaves, reduced root...
A high preoperative peripheral blood neutrophil-to-lymphocyte ratio (NLR) has been reported to be a predictor of poor survival in patients with various cancers. The aim this study was evaluate the predictive significance NLR undergoing hepatectomy for intrahepatic cholangiocarcinoma (ICC). From 2005 2011, 322 who underwent ICC were enrolled retrospective study. Clinicopathological parameters, including NLR, evaluated identify predictors overall and recurrence-free after hepatectomy. best...
Abstract Ten eleven translocation (TET) enzymes convert 5-methylcytosine (5-mC) to 5-hydroxy-methylcytosine (5-hmC) and have crucial roles in biological pathological processes by mediating DNA demethylation, however, the functional role of this epigenetic mark related hepatocellular carcinoma (HCC) progression remains unknown. Here, we demonstrated that TET-family downregulation was one likely mechanism underlying 5-hmC loss HCC. We found miR-29a overexpression increased methylation...
Early recurrence of hepatocellular carcinoma (HCC) after curative resection is common. However, the association between genetic mechanisms and early HCC recurrence, especially in Chinese patients, remains largely unknown.We performed whole-genome sequencing (49 cases), whole-exome (18 deep targeted (115 cases) on 182 primary samples. Focusing WNK2, we used Sanger qPCR to evaluate all coding exons copy numbers that gene an additional 554 We also explored functional effect mechanism WNK2 tumor...
Background & AimsCancer stemness and immune evasion are closely associated play critical roles in tumor development resistance to immunotherapy. However, little is known about the underlying molecular mechanisms that coordinate this association.MethodsThe expressions of heterogeneous nuclear ribonucleoprotein M (HNRNPM) 240 hepatocellular carcinoma (HCC) samples, public databases, liver databases were analyzed. Chromatin immunoprecipitation assays performed explore associations between...
Objective:The low clinical utility of immune checkpoint inhibitors (ICIs) against PD-1 or PD-L1 has recently been associated with the activation Wnt/β-catenin signaling pathway in hepatocellular carcinoma (HCC), which promotes tumor escape and resistance to anti-PD-1/PD-L1 therapy.Hence, we aimed fabricate a supramolecular peptide could target coupled ICIs blockage therapy for optimizing HCC immunotherapy.Methods: A racemic spherical termed sBBI&PDP nanoparticle was constructed by...
Importance BRAF variants are associated with tumor progression; however, the prevalence of variant subtypes and their association disease characteristics, prognosis, targeted therapy response in patients intrahepatic cholangiocarcinoma (ICC) largely unknown. Objective To explore ICC. Design, Setting, Participants In this cohort study, 1175 who underwent curative resection for ICC from January 1, 2009, through December 31, 2017, were evaluated at a single hospital China. Whole-exome...
Calpain small subunit 1 (Capn4) has been identified as a major gene that promotes metastasis of hepatocellular carcinoma (HCC). However, the mechanism by which Capn4 progression HCC is not understood. In this study, we found expression was increased in highly metastatic cell lines and tumour tissue from patients compared to healthy patient tissue. Over-expression cells enhanced growth vitro invasiveness, tumourigenicity lung vivo. Protein microarray analyses showed multiple proteins...
Abstract Increasing numbers of evidences have demonstrated that microRNAs (miRNAs) are implicated in metastasis and progression hepatocellular carcinoma (HCC). However, their detailed expression levels actual functions HCCs not been fully clarified yet. Results from our recent study revealed some miRNAs were particularly related to HCCs. As one these newly found miRNAs, miR-501-3p showed highly involve into metastatic process Here we reported the was decreased both HCC cell lines tissue...