A5012420216- Cholangiocarcinoma and Gallbladder Cancer Studies
- Chromosomal and Genetic Variations
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Plant Pathogens and Resistance
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Circular RNAs in diseases
- Viral-associated cancers and disorders
- Genomics and Chromatin Dynamics
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogens and Fungal Diseases
- Plant Molecular Biology Research
- Immunotherapy and Immune Responses
- Tissue Engineering and Regenerative Medicine
- Hepatocellular Carcinoma Treatment and Prognosis
- Flowering Plant Growth and Cultivation
- Plant Virus Research Studies
- Congenital heart defects research
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Cancer Mechanisms and Therapy
- Pluripotent Stem Cells Research
Michigan State University
2019-2025
Fudan University
2017-2024
Zhongshan Hospital
2019-2024
The First Affiliated Hospital, Sun Yat-sen University
2020-2024
Sun Yat-sen University
2020-2024
Nanjing Forestry University
2016-2024
Burning Rock Biotech (China)
2024
Tianjin Medical University
2024
Second Hospital of Tianjin Medical University
2024
Tianjin University
2024
There is growing evidence that single-stranded, circular RNA (circRNA) plays a key role in the development of certain cancers, including hepatocellular carcinoma (HCC). It less clear, however, what circRNA HCC metastasis.In this study, through sequencing, we identified circRNA: circASAP1 (a derived from exons 2 and 3 ASAP1 gene, hsa_circ_0085616), which associated with pulmonary metastasis after curative resection patients HCC. CircASAP1 was overexpressed cell lines high metastatic potential...
Tumor-associated neutrophils (TANs) play a crucial role in tumor development and progression the cancer microenvironment. Despite increased understanding of TAN contributions to hepatocellular carcinoma (HCC) prognosis, direct interaction between TANs HCC cells is not fully understood. In this study, we tested effect on vitro vivo investigated mechanism them. Our results showed that secreted bone morphogenetic protein 2 transforming growth factor beta triggered microRNA 301b-3p (miR-301-3p)...
Abstract Background and Aims Macrophages are prominent components of solid tumors exhibit distinct functions in different tumor microenvironments. Exosomes emerging as necessary mediators the cross‐talk between cells microenvironment. However, underlying mechanisms exosomes involving into crosstalk macrophages during disease progression intrahepatic cholangiocarcinoma (ICC) have not been yet fully realized. Approach Results We found that ICC tissues up‐regulated expression levels...
Background Tumor-associated neutrophils (TANs) and macrophages (TAMs) can each influence cancer growth metastasis, but their combined effects in intrahepatic cholangiocarcinoma (ICC) remain unclear. Methods We explored the distributions of TANs TAMs patient-derived ICC samples by multiplex immunofluorescent staining tested separate on vitro vivo. then investigated mechanistic basis using PCR array, western blot analysis ELISA experiments. Finally, we validated our results a tissue microarray...
Abstract Potato (Solanum tuberosum) is the third most important food crop in world. tubers must be stored at cold temperatures to minimize sprouting and losses due disease. However, strongly induce expression of potato vacuolar invertase gene (VInv) cause reducing sugar accumulation. This process, referred as “cold-induced sweetening,” a major postharvest problem for industry. We discovered that cold-induced VInv controlled by 200 bp enhancer, VInvIn2En, located its second intron. identified...
Early recurrence of hepatocellular carcinoma (HCC) after curative resection is common. However, the association between genetic mechanisms and early HCC recurrence, especially in Chinese patients, remains largely unknown.We performed whole-genome sequencing (49 cases), whole-exome (18 deep targeted (115 cases) on 182 primary samples. Focusing WNK2, we used Sanger qPCR to evaluate all coding exons copy numbers that gene an additional 554 We also explored functional effect mechanism WNK2 tumor...
The karyotype represents the basic genetic make-up of a eukaryotic species. Comparative cytogenetic analysis related species based on individually identified chromosomes has been conducted in only few plant groups and not yet woody plants. We have developed complete set 19 chromosome painting probes reference genome model Populus trichocarpa. Using sequential fluorescence situ hybridization we were able to identify all poplar same metaphase cells, which led development karyotypes...
Super-enhancers (SEs) are exceptionally large enhancers and recognized to play prominent roles in cell identity mammalian species. We surveyed the genomic regions containing clusters of accessible chromatin (ACRs) marked by deoxyribonuclease (DNase) I hypersensitivity Arabidopsis thaliana . identified a set 749 putative SEs, which have minimum length 1.5 kilobases represent top 2.5% largest ACR clusters. demonstrate that associating with these SEs were more sensitive DNase than other...
Importance BRAF variants are associated with tumor progression; however, the prevalence of variant subtypes and their association disease characteristics, prognosis, targeted therapy response in patients intrahepatic cholangiocarcinoma (ICC) largely unknown. Objective To explore ICC. Design, Setting, Participants In this cohort study, 1175 who underwent curative resection for ICC from January 1, 2009, through December 31, 2017, were evaluated at a single hospital China. Whole-exome...
Potato (Solanum tuberosum) is the third-most important food crop in world. Although potato genome has been fully sequenced, functional genomics research of lags behind that other major crops, largely due to lack a model experimental line. Here, we present diploid line, 'Jan,' which possesses all essential characteristics for facile studies. Jan exhibits high level homozygosity after seven generations self-pollination. vigorous, highly fertile and produces tubers with outstanding traits....
<h3>Importance</h3> <i>KRAS</i>variants are associated with tumor progression; however, the prevalence of<i>KRAS</i>variant subtypes and their association survival recurrence in patients intrahepatic cholangiocarcinoma (ICC) after curative resection largely unknown. <h3>Objective</h3> To explore prognostic ICC. <h3>Design, Setting, Participants</h3> In this cohort study, who underwent for ICC from January 2009 through December 2016 at a single hospital China were recruited, whole-exome...
Abstract Patients with hepatocellular carcinoma (HCC) have poor long-term survival following curative resection because of the high rate tumor early recurrence. Little is known about trajectory genomic evolution from primary to early-recurrent HCC. In this study, we performed whole-genome sequencing (WGS) on 40 pairs and hepatitis B virus (HBV)-related HCC tumors patients who received resection, four whose recurrent were extensively sampled. We identified two recurrence patterns: de novo...
Abstract The majority of sequenced genomes in the monocots are from species belonging to Poaceae, which include many commercially important crops. Here, we expand number 4 related cyperids: Carex cristatella and scoparia Cyperaceae Juncus effusus inflexus Juncaceae. high-quality, chromosome-scale genome sequences these cyperids were assembled by combining whole-genome shotgun sequencing Nanopore long reads, Illumina short Hi-C data. Some members Juncaceae known possess holocentric...
Most infarctions occur in the left anterior descending coronary artery and cause myocardium damage of ventricle. Although current pluripotent stem cells (PSCs) directed cardiac differentiation techniques are able to generate fetal-like human cardiomyocytes, isolation pure ventricular cardiomyocytes has been challenging. For repairing damage, we aimed establish a highly efficient purification system obtain homogeneous prepare engineered heart muscles dish. The used TALEN-mediated genomic...
Plasmacytoid dendritic cells (pDCs) are present in various primary and metastatic human neoplasms; however, their clinical significance intrahepatic cholangiocarcinoma is not clear.To evaluate pDCs' distributions around tumors as well potential function predictive value for prognosis patients undergoing curative resection, we performed immunohistochemistry to examine the expression of pDC marker BDCA2, CD3, CD4, CD8 Foxp3 intratumoral peritumoral tissues from 359 with compared prognostic...
Backgrounds/Aims: Intrahepatic cholangiocarcinoma (ICC) is a highly desmoplastic tumor with poor prognosis even after curative resection. We investigated the associations between composition of ICC stroma and immune cell infiltration aimed to develop stromal-immune signature predict in surgically treated ICC.Methods: recruited 359 patients performed immunohistochemistry detect α-smooth muscle actin (α-SMA), CD3, CD4, CD8, Foxp3, CD68, CD66b. Aniline was used stain collagen deposition....
Abstract Structural variations (SVs) influence the development and progression of multiple types cancer. The genes affected by SVs in hepatocellular carcinoma (HCC) their contribution to tumor growth metastasis remain unknown. In this study, through whole-genome sequencing (WGS), we identified MACROD2 as gene most frequently SVs, which were associated with low expression levels. Low was predictive recurrence poor overall survival. decreased HCC cell lines, especially those high metastatic...
Accumulating evidence indicates that tumor heterogeneity is characterized by distinct immunosubtypes. However, prior studies have mainly focused on the functions of T cells. The role tumor-infiltrating B cells in microenvironment hepatocellular carcinoma (HCC) requires further investigation.We conducted an integrative analysis single cell RNA sequencing (scRNA-seq) datasets HCC samples from Gene Expression Omnibus database. We analyzed features normal liver tissue and HCC. Additionally, we a...