A5023303944- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Flowering Plant Growth and Cultivation
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Bioenergy crop production and management
- Plant Pathogens and Resistance
- Plant Genetic and Mutation Studies
- Powdery Mildew Fungal Diseases
- Plant Gene Expression Analysis
- Genetic diversity and population structure
- Plant nutrient uptake and metabolism
- Ecology and Conservation Studies
- Botanical Studies and Applications
- Plant biochemistry and biosynthesis
- Genomics and Chromatin Dynamics
- Plant responses to water stress
- Seed Germination and Physiology
- Yeasts and Rust Fungi Studies
- Medicinal Plant Pharmacodynamics Research
- RNA modifications and cancer
- Nuts composition and effects
Nanjing Forestry University
2015-2024
Ministry of Education of the People's Republic of China
2022
Sustainable Innovation (Sweden)
2016-2017
Wageningen University & Research
2015
The karyotype represents the basic genetic make-up of a eukaryotic species. Comparative cytogenetic analysis related species based on individually identified chromosomes has been conducted in only few plant groups and not yet woody plants. We have developed complete set 19 chromosome painting probes reference genome model Populus trichocarpa. Using sequential fluorescence situ hybridization we were able to identify all poplar same metaphase cells, which led development karyotypes...
Centromeres in most multicellular eukaryotes are composed of long arrays repetitive DNA sequences. Interestingly, several transposable elements, including the well-known terminal repeat centromeric retrotransposon maize (CRM), were found to be enriched functional centromeres marked by histone H3 (CENH3). Here, we report a interspersed nuclear element (LINE), Celine, Populus species. Celine has colonized preferentially CENH3-associated chromatin every poplar chromosome, with 84% elements...
Poplar was one of the first woody species whose individual chromosomes could be identified using chromosome specific painting probes. Nevertheless, high-resolution karyotype construction remains a challenge. Here, we developed based on meiotic pachytene Populus simonii which is Chinese native with many excellent traits. This anchored by oligonucleotide (oligo)-based probes, centromere-specific repeat (Ps34), ribosomal DNA, and telomeric DNA. We updated known formula for P. to 2n = 2x 38 26m...
Lilium regale E.H. Wilson is endemic to a narrow geographic area in the Minjiang River valley southwestern China, and considered an important germplasm for breeding commercially valuable lily varieties, due its vigorous growth, resistance diseases tolerance low moisture. We analyzed genetic diversity of eight populations L. sampled across entire natural distribution range species using Inter-Simple Sequence Repeat markers. The (expected heterozygosity= 0.3356) was higher than those reported...
The camphor tree (Cinnamomum camphora (L.) Presl.) is the representative species of subtropical evergreen broadleaved forests in eastern Asia and an important raw material for essential oil production worldwide. Although MYBs have been comprehensively characterized their functions partially resolved many plants, it has not explored C. camphora. In this study, 121 CcMYBs were identified on 12 chromosomes whole genome found that mainly expanded by segmental duplication. They divided into 28...
Agrobacterium-mediated genetic transformation is well established in the model grass Brachypodium distachyon. However, most protocols employ immature embryos because of their better regenerative capacity. A major problem associated with embryo system that they are available only during a limited time window growing plants. In this study, we have developed an optimized protocol utilizes mature embryos. We adopted seed shearing and photoautotrophic rooting (PR) callus induction root...
Brachypodium distachyon, a new monocotyledonous model plant, has received wide attention in biological research due to its small genome and numerous genetic resources. Codon usage bias is an important feature of genes genomes, it can be used transgenic evolutionary studies. In this study, the nucleotide compositions patterns codon were calculated using W. Additionally, ENC plot, Parity rule 2 correspondence analyses explore major factors influencing patterns. The numbers hydrogen bonds skews...
Two Asiatic lily cultivars, 'Golden Horn', 'Pollyanna', and their six progenies were analyzed using Fluorescence in situ hybridization (FISH) with 45S rDNA as probe. It was shown that five diploid (2n=2x=24), one progeny triploid (2n=3x=36), We observed a total of 11 signals on chromosome No 1, 2, 3, 4, 7 10 occurred 6, 'Pollyanna'. loci variable 3 6 these two cultivars. Chromosome Horn' contained which at the centromere long arm, while locus 'Pollyanna' arm. In addition, 1 located near...
Karyotype comparison and fluorescence in situ hybridization (FISH) were conducted to analyze the wild Lilium species distributed China. The karyotype results revealed that all except lancifolium (2 n = 3 X 36) diploid had two pairs of metacentric or submetacentric chromosomes. karyotypes are similar. FISH analysis there 5–12 45S rRNA gene loci dispersed on chromosomes 14 species, 15 detected triploid L. . Most signals long arms centromeric regions. Three samples brownii [Hubei, China (lat....
Lily ( Lilium L.) species produce among the most important cut flowers worldwide. China has ≈55 of . Although many plants from this genus have been used in hybridization efforts, their cytology remained unclear. The goal current study was to characterize chromosomes rosthornii Diels. Root tips were Giemsa C-banding, propidium iodide (PI) banding, and 45S rDNA locations. karyotype L. belongs type 3B. C-banding revealed polymorphic banding patterns with following formula: 2n = 24 CI 4C + 14CI...
Abstract A technique that produces large numbers of good quality pachytene chromosome preparations has been developed for Populus . Anthers at the stage meiosis are used as materials. There two main modifications in our method relative to traditional squashing address challenges thick cytoplasm observed during phase presents. One is temperature squashing, i.e., slide placed on a 52°C heater squashing. The other removal cover slip using 45% acetic acid. © 2016 by John Wiley & Sons, Inc.