A5101535276- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Radioactivity and Radon Measurements
- Chemical Synthesis and Analysis
- Computational Drug Discovery Methods
- Renal function and acid-base balance
- Innovative Microfluidic and Catalytic Techniques Innovation
- Trace Elements in Health
- RNA regulation and disease
- Plant nutrient uptake and metabolism
- Research in Cotton Cultivation
- Odor and Emission Control Technologies
- Plant tissue culture and regeneration
- Urban Agriculture and Sustainability
- Inflammasome and immune disorders
- TGF-β signaling in diseases
- Tannin, Tannase and Anticancer Activities
- Renal Diseases and Glomerulopathies
- Silicon Effects in Agriculture
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Light effects on plants
Chinese Academy of Sciences
2024
University of Chinese Academy of Sciences
2024
Yangzhou University
2015-2024
First People's Hospital of Chongqing
2024
Beijing Forestry University
2020-2024
Kunming Institute of Botany
2024
Institute of Geographic Sciences and Natural Resources Research
2024
Ministry of Education of the People's Republic of China
2022
Norwich University
2002
CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated temperature sensitive in plants, but a lack of comprehensive understanding on Cas12a sensitivity plant cells has hampered effective application nucleases editing. We compared AsCas12a, FnCas12a, and LbCas12a for their efficiencies non-homologous end joining (NHEJ) repair profiles at four different temperatures rice. found AsCas12a more to it...
Summary CRISPR ‐Cas9 and Cas12a are two powerful genome editing systems. Expression of in plants is typically achieved with a mixed dual promoter system, which Cas protein expressed by Pol II guide RNA species‐specific III such as U6 or U3. To achieve coordinated expression compact vector packaging, it desirable to express both components under single promoter. Previously, we demonstrated first‐generation transcript unit ( STU )‐Cas9 ‐Cas9‐ RZ , based on hammerhead ribozyme for processing s...
Summary PAM‐relaxed Cas9 nucleases, cytosine base editors and adenine are promising tools for precise genome editing in plants. However, their genome‐wide off‐target effects largely unexplored. Here, we conduct whole‐genome sequencing (WGS) analyses of transgenic plants edited by xCas9, Cas9‐NGv1, Cas9‐NG, SpRY, nCas9‐NG‐PmCDA1, nSpRY‐PmCDA1 nSpRY‐ABE8e rice. Our results reveal that nuclease editors, when coupled with the same guide RNA (gRNA), prefer distinct gRNA‐dependent sites. De novo...
Summary Cytosine base editors (CBEs) can install a predefined stop codon at the target site, representing more predictable and neater method for creating genetic knockouts without altering genome size. Due to enhanced predictability of editing outcomes, it is also efficient obtain homozygous mutants in first generation. With recent advancement CBEs on improved activity, purify specificity plants animals, has become appealing technology generating knockouts. However, there lack design tools...
Structure-based drug design (SBDD) aims to generate 3D ligand molecules that bind specific protein targets. Existing deep generative models including diffusion have shown great promise for SBDD. However, it is complex capture the essential protein-ligand interactions exactly in space molecular generation. To address this problem, we propose a novel framework, namely Binding-Adaptive Diffusion Models (BindDM). In BindDM, adaptively extract subcomplex, part of binding sites responsible...
Abstract Background The development of cotton fiber is regulated by the orchestrated binding regulatory proteins to cis -regulatory elements associated with developmental genes. cis–trans dynamics occurred throughout course are elusive. Here we generated genome-wide high-resolution DNase I hypersensitive sites (DHSs) maps understand mechanisms ovule and development. Results We site (DHS) profiles from ovules at 0 3 days post anthesis (DPA) fibers 8, 12, 15, 18 DPA. obtained a total 1185...
This review synthesizes the current understanding on dynamic influence of light developmental morphology woody plants. It explores regulatory effects photosynthesis and photomorphogenesis in response to varying conditions including intensity, quality, photoperiodicity, their subsequent impact plant growth architecture. Additionally, this elucidates role circadian system synchronizing internal rhythms with external cycles, a process mediated by photoreceptors such as PHYTOCHROME A (PHYA) B...
Abstract Background Lysimachia christinae Hance (LCH) is a traditional medicine used to treat gallstone disease and cholecystitis. Despite its known anti‐inflammatory choleretic effects, quality has not been extensively evaluated. Objective In this study, we aimed establish reliable evaluation method for LCH via fingerprint, spectrum–effect relationship, quantitative analyses of multicomponents by single marker (QAMS). Methods First, the fingerprints activities 14 batches were determined....
High concentrations of Na+ in saline soil impair plant growth and agricultural production. Protein tyrosine phosphorylation is crucial many cellular regulatory mechanisms. However, mechanisms protein phosphatases (PTPs) controlling responses to abiotic stress remain limited. We report here the identification a Tyrosine (Tyr)-specific phosphatase, PdPTP1, from NE19 (Populus nigra × (P. deltoides P. nigra). Transcript levels PdPTP1 were upregulated significantly by NaCl treatment oxidative...
Centromeres in most multicellular eukaryotes are composed of long arrays repetitive DNA sequences. Interestingly, several transposable elements, including the well-known terminal repeat centromeric retrotransposon maize (CRM), were found to be enriched functional centromeres marked by histone H3 (CENH3). Here, we report a interspersed nuclear element (LINE), Celine, Populus species. Celine has colonized preferentially CENH3-associated chromatin every poplar chromosome, with 84% elements...
Reversible watermark is a kind of fragile watermark, and any changes will lead to the change or loss watermarking information. The modified sensitivity reversible can be used for integrity verification grid map. When content carrier data suspected, type manipulation attack suffered judged through extracting situation. algorithm proposed by this paper aims at characteristics map, reduce impact low correlation adjacent pixels, pixel values are distributed more intensively after disposal...
Structure-based drug design (SBDD) aims to generate 3D ligand molecules that bind specific protein targets. Existing deep generative models including diffusion have shown great promise for SBDD. However, it is complex capture the essential protein-ligand interactions exactly in space molecular generation. To address this problem, we propose a novel framework, namely Binding-Adaptive Diffusion Models (BindDM). In BindDM, adaptively extract subcomplex, part of binding sites responsible...
Combining multiple cameras in a bigger multi-camera system give the opportunity to realize novel concepts (e.g. omnidirectional video, view interpolation) real-time. The better quality, more data that is needed be captured. As has direct impact on storage space and communication bandwidth, it preferable reduce load by compressing size. This cannot come at expense of latency, because main requirement real-time processing for video applications. Also, all image details need preserved improving...
PAM-relaxed Cas9 nucleases, cytosine base editors and adenine are promising tools for precise genome editing in plants. However, their genome-wide off-target effects largely undetermined. Here, we conduct whole-genome sequencing (WGS) analyses of transgenic plants edited by xCas9, Cas9-NGv1, Cas9-NG, SpRY, nCas9-NG-PmCDA1, nSpRY-PmCDA1 nSpRY-ABE8e rice. Our results reveal different guide RNA (gRNA)-dependent with editors. De novo generated new gRNAs SpRY lead to additional but not...