A5080996370- CRISPR and Genetic Engineering
- Land Use and Ecosystem Services
- Urban Transport and Accessibility
- RNA and protein synthesis mechanisms
- Coastal wetland ecosystem dynamics
- Impact of Light on Environment and Health
- RNA regulation and disease
- Air Quality and Health Impacts
- Plant Virus Research Studies
- Coastal and Marine Dynamics
- Antioxidant Activity and Oxidative Stress
- Plant biochemistry and biosynthesis
- Remote Sensing in Agriculture
- Geology and Paleoclimatology Research
- Chromosomal and Genetic Variations
- Urban Green Space and Health
- Regulation and Compliance Studies
- Atherosclerosis and Cardiovascular Diseases
- Plant Molecular Biology Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Belt and Road Initiative
- Regional Development and Environment
- Local Economic Development and Planning
- Insect symbiosis and bacterial influences
- Global Urban Networks and Dynamics
Nanning Normal University
2022-2025
Nanjing University
2018-2022
Qingdao Municipal Hospital
2014-2022
Qingdao University
2014-2022
Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application
2020-2021
University of Electronic Science and Technology of China
2018-2020
Linyi City Chinese Medicine Hospital
2020
Shanghai Normal University
2018
Beijing University of Chinese Medicine
2015-2016
Weatherford College
2014
Targeting specificity has been a barrier to applying genome editing systems in functional genomics, precise medicine and plant breeding. In plants, only limited studies have used whole-genome sequencing (WGS) test off-target effects of Cas9. The cause numerous discovered mutations is still controversial. Furthermore, WGS-based analysis Cpf1 (Cas12a) not reported any higher organism date.
Summary CRISPR ‐Cas9 and Cas12a are two powerful genome editing systems. Expression of in plants is typically achieved with a mixed dual promoter system, which Cas protein expressed by Pol II guide RNA species‐specific III such as U6 or U3. To achieve coordinated expression compact vector packaging, it desirable to express both components under single promoter. Previously, we demonstrated first‐generation transcript unit ( STU )‐Cas9 ‐Cas9‐ RZ , based on hammerhead ribozyme for processing s...
Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt with various metabolism pathways. A number of transcription family have been found be response, and NAM, ATAF CUC (NAC) factors thought act as active regulators during stress, especially stress. In this study, we detected rice NAC coding gene, OsNAC041, confirmed it influenced germination seeds under tolerance plants. OsNAC041 was primarily expressed leaves located...
Africa has been undergoing a rapid urbanization process, which is critical to the achievement of 11th Sustainable Development Goal (SDG11). Using population density data from LandScan, we proposed density-based thresholding method generate urban land and in 2001 2019, were further applied detect spatiotemporal characteristics Africa’s urbanization. The results showed that have both grown rapidly Africa, increased by about 5.92% 4.91%, respectively. top three countries with most intense...
As an important part of the terrestrial ecosystem, vegetation dynamics are subject to impacts from both climate change and human activities. Clarifying driving mechanisms variation is great significance for regional ecological protection achieving sustainable development goals. Here, net primary productivity (NPP) was used investigate spatiotemporal variability 2000 2020 in East Africa, its correlations with factors. Furthermore, we utilized partial derivatives analysis set up different...
China’s Yellow (Bohai) Sea bird habitat is an important ecological region. Its unique ecology and challenges provide rich resources for research study. Our course design concept supported by AI technology, improves students’ abilities through innovative functions such as dynamic data support, personalized learning paths, immersive study experience, diversified evaluation mechanisms. The content revolves around the “human–land coordination concept”, including pre-trip thinking, during trip,...
Exploring the urban spatial pattern and expansion characteristics of African countries under shared socioeconomic pathways (SSPs) is crucial to optimizing development in Africa ensuring ecological sustainability. We use land panel data least squares dummy variable regression method predict increment from 2030 2060, we FLUS model simulate layout analyze perspective relationship between population density expansion. The results show that space will a significant trend 2020 with stronger growth...
With the rapid development of industrialization and urbanization, air pollution is becoming increasingly serious. Accurate prediction PM2.5 concentration great significance to environmental protection public health. Our study takes Nanning urban area, which has unique geographical, climatic source characteristics, as object. Based on dual-time resolution raster data China High-resolution High-quality Dataset (CHAP) from 2012 2023, carried out using SARIMA, Prophet LightGBM models. The...
In view of the challenges brought by a complex environment, diverse data sources and urban development needs, our study comprehensively reviews application algorithms in residential vacancy rate observation. First, we explore definition measurement rate, pointing out difficulties accurately defining vacant houses obtaining reliable data. Then, introduce various such as traditional statistical learning, machine deep learning ensemble analyze their applications The algorithm builds prediction...
Africa’s PM2.5 pollution has become a security hazard, but the understanding of varying effects urbanization on driven mechanisms concentrations under rapid remains largely insufficient. Compared with direct impact, spillover effect in adjacent regions was underestimated. Urbanization is highly multi-dimensional phenomenon and previous studies have rarely distinguished different driving influence interactions Africa. This study combined grid administrative units to explore spatio-temporal...
Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents compact CRISPR expression system has many advantages for different applications. In order to make this routine in plant biology, engineering efforts are needed developing optimizing such transcript unit (STU) systems genome editing.To develop novel intron-based STU (iSTU) (STU 3.0), we first evaluated three introns from species carrying using an enhanced green fluorescence protein (eGFP)...
The high-quality development of small towns in Guangxi is crucial for regional economic balance and sustainable development. This research conducts an in-depth exploration systematic construction validation analysis the Guangxi. Guided by new concepts innovation, coordination, green, openness, sharing, a comprehensive evaluation system established to assess levels 70 from 2005 2022. findings are as follows: 1. revealed significant growth these dimensions over study period, highlighting...
Chinese dragon's blood, the red resin of Dracaena cochinchinensis, one renowned traditional medicines, has been used to facilitate blood circulation and disperse stasis for thousands years. Phenolic compounds are considered be responsible its main biological activities. In this study, total phenolic were made into capsule (Longxuetongluo Capsule, LTC) their effects on abnormal hemorheological properties examined by high fat diet (HFD) induced ApoE-/- mice. Compared model group, LTC recovered...
As an important step enhancing regional innovation, researches on collaborative innovation have attracted much more attention recently. One significant reason is that cities can get excessive benefits while they take activities. Based the theories of geography, this paper takes Yangtze River Delta (YRD) Urban Agglomeration as a case study and measures capacity from actors by adopting catastrophe progression model. Then basis, depicts spatial pattern benefit allocation using coupling degree...
Genome editing technology represented by CRISPR-Cas9 had been widely used in many biological fields such as gene function analysis, therapy, and crop improvement. Due to the complexity of eukaryotic genome, there was still a problem that unstable efficiency with large variability among different target sites. It important further improve system whole genome. In this study, based on previous single transcription unit genome (STU-SpCas9), using ubiquitin-associated domain (UBA) enhance...
Suburban rural landscape multifunction has received increasing attention from scholars due to its high demand and impact on main urban areas. However, few studies have been focused suburban because of data constraints. The present study quantified the four services based ecological service system, i.e., regulating function (RF), provision (PF), culture (CF), support (SF), determined interaction through Spearman correlation coefficient, ultimately identified hotspots dominant functions...
Mangroves are endemic forest communities in tropical and subtropical coastal zones. China’s Hainan Island is very rich mangrove plant species. In recent years, due to climate change human disturbance, the living environment of many distribution areas has been seriously threatened. This study used land satellite series remote sensing images from 1990 2020 monitor coverage area changes mangroves on Island. The spatial pattern trend were explored using standard deviation ellipse method,...
Africa has been experiencing a rapid urbanization process, which may lead to an increase in unsustainable land use and urban poverty. Assessing the spatiotemporal characteristics of dynamics is especially important needed for sustainable development Africa. Satellite-based nighttime light (NTL) data are widely used monitor growth from global local scales. In this study, patterns across were analyzed discussed using stable datasets obtained DMSP/OLS (the Defense Meteorological Satellite...
Egypt has an important geostrategic position as it is located in the three circles of Arab world, African continent and Islamic world simultaneously. key region Belt Road Initiative (BRI), with leading radiating effects on Africa West Asia. The study Sino-Egyptian geo-relations great significance for promoting construction BRI. This paper attempted to explore evolution process driving mechanism during 2000–2021 based event data analysis path future development. results indicate that: (1)...