A5100728526- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Cell death mechanisms and regulation
- RNA regulation and disease
- Insect symbiosis and bacterial influences
- Plant Stress Responses and Tolerance
- Flavonoids in Medical Research
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- NF-κB Signaling Pathways
- Cancer-related molecular mechanisms research
- Plant Gene Expression Analysis
- Wheat and Barley Genetics and Pathology
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Pancreatitis Pathology and Treatment
- Innovation and Socioeconomic Development
- Arsenic contamination and mitigation
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Diabetes and associated disorders
- Electrochemical Analysis and Applications
Sichuan University
2016-2025
Southwest University
2024-2025
Tianjin University of Science and Technology
2023-2025
First Affiliated Hospital of Xi'an Jiaotong University
2023-2024
Zhejiang University
2023-2024
University of Electronic Science and Technology of China
2015-2024
First Affiliated Hospital of Nanchang University
2018-2024
Zero to Three
2024
West China Second University Hospital of Sichuan University
2010-2023
Nanchang University
2018
The relative ease, speed, and biological scope of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for genomic manipulations are revolutionizing virtually all areas molecular biosciences, including functional genomics, genetics, applied biomedical research, agricultural biotechnology. In plant systems, however, a number hurdles currently exist that limit this technology from reaching its full potential. For example,...
Targeting specificity has been a barrier to applying genome editing systems in functional genomics, precise medicine and plant breeding. In plants, only limited studies have used whole-genome sequencing (WGS) test off-target effects of Cas9. The cause numerous discovered mutations is still controversial. Furthermore, WGS-based analysis Cpf1 (Cas12a) not reported any higher organism date.
CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated temperature sensitive in plants, but a lack of comprehensive understanding on Cas12a sensitivity plant cells has hampered effective application nucleases editing. We compared AsCas12a, FnCas12a, and LbCas12a for their efficiencies non-homologous end joining (NHEJ) repair profiles at four different temperatures rice. found AsCas12a more to it...
MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in plant development and stress responses. Loss-of-function analysis of miRNA genes has been traditionally challenging due to lack appropriate knockout tools. In this study, single (OsMIR408 OsMIR528) gene families (miR815a/b/c miR820a/b/c) rice were targeted by CRISPR-Cas9. We showed strand conformation polymorphism (SSCP) is a more reliable method than restriction fragment length (RFLP) for identifying CRISPR-Cas9...
Abstract CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic regions. Comprehensive engineering requires simultaneous of multiple genes at defined locations. Here, to expand the scope Cas12a, we screen nine Cas12a orthologs that have not been demonstrated in plants, and identify six, ErCas12a, Lb5Cas12a, BsCas12a, Mb2Cas12a, TsCas12a MbCas12a, possess high activity rice. Among them, Mb2Cas12a stands out with efficiency tolerance low temperature. An engineered...
Summary CRISPR ‐Cas9 and Cas12a are two powerful genome editing systems. Expression of in plants is typically achieved with a mixed dual promoter system, which Cas protein expressed by Pol II guide RNA species‐specific III such as U6 or U3. To achieve coordinated expression compact vector packaging, it desirable to express both components under single promoter. Previously, we demonstrated first‐generation transcript unit ( STU )‐Cas9 ‐Cas9‐ RZ , based on hammerhead ribozyme for processing s...
Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt with various metabolism pathways. A number of transcription family have been found be response, and NAM, ATAF CUC (NAC) factors thought act as active regulators during stress, especially stress. In this study, we detected rice NAC coding gene, OsNAC041, confirmed it influenced germination seeds under tolerance plants. OsNAC041 was primarily expressed leaves located...
Rice (Oryza sativa) responds to various abiotic stresses during growth. Plant-specific NAM, ATAF1/2, and CUC2 (NAC) transcription factors (TFs) play an important role in controlling numerous vital growth developmental processes. To date, 170 NAC TFs have been reported rice, but their roles remain largely unknown. Herein, we discovered that the TF OsNAC006 is constitutively expressed regulated by H2O2, cold, heat, abscisic acid (ABA), indole-3-acetic (IAA), gibberellin (GA), NaCl,...
Summary Cytosine base editors (CBEs) are great additions to the expanding genome editing toolbox. To improve C‐to‐T in plants, we first compared seven cytidine deaminases BE3‐like configuration rice. We found A3A/Y130F‐CBE_V01 resulted highest efficiency both rice and Arabidopsis . Furthermore, demonstrated this A3A/Y130F deaminase could be used iSpyMacCas9‐mediated at A‐rich PAMs. showcase its applications, applied for multiplexed generate microRNA‐resistant mRNA transcripts as well...
Summary PAM‐relaxed Cas9 nucleases, cytosine base editors and adenine are promising tools for precise genome editing in plants. However, their genome‐wide off‐target effects largely unexplored. Here, we conduct whole‐genome sequencing (WGS) analyses of transgenic plants edited by xCas9, Cas9‐NGv1, Cas9‐NG, SpRY, nCas9‐NG‐PmCDA1, nSpRY‐PmCDA1 nSpRY‐ABE8e rice. Our results reveal that nuclease editors, when coupled with the same guide RNA (gRNA), prefer distinct gRNA‐dependent sites. De novo...
Abstract Cytokinin (CTK) is an important plant hormone that promotes cell division, controls differentiation, and regulates a variety of growth development processes. oxidase/dehydrogenase (CKX) irreversible cytokinin‐degrading enzyme affects by regulating the dynamic balance CTKs synthesis degradation. There are presumed 11 members CKX gene family in rice ( Oryza sativa L.), but limited have been reported. In this study, based on CRISPR‐Cas9 CRISPR‐Cas12a genome‐editing technology, we...
Abstract Cytosine base editors (CBEs) and adenine (ABEs) enable precise C-to-T A-to-G edits. Recently, ABE8e, derived from TadA-8e, enhances edits in mammalian cells plants. Interestingly, TadA-8e can also be evolved to confer editing. This study compares engineered CBEs rice tomato cells, identifying TadCBEa, TadCBEd, TadCBEd_V106W as efficient with high purity a narrow editing window. A dual editor, TadDE, promotes simultaneous Multiplexed TadCBEa TadDE is demonstrated transgenic rice, no...
Summary CRISPR‐Cas9 is widely used for genome editing, but its PAM sequence requirements limit efficiency. In this study, we explore Faecalibaculum rodentium Cas9 (FrCas9) plant especially in rice. FrCas9 recognizes a concise 5′‐NNTA‐3′ PAM, targeting more abundant palindromic TA sites genomes than the 5′‐NGG‐3′ of most popular SpCas9. shows cleavage activities at all tested with editing outcomes sharing same characteristics typical system. induces high‐efficiency targeted mutagenesis stable...
Drought decreases crop productivity more than any other type of environmental stress. Transcription factors (TFs) play crucial roles in regulating plant abiotic stress responses. The Arabidopsis thaliana gene DREB1A/CBF3, encoding a stress-inducible TF, was introduced into Salvia miltiorrhiza Ectopic expression AtDREB1A resulted increased drought tolerance, and transgenic lines had higher relative water content Chl content, exhibited an photosynthetic rate when subjected to plants generally...