A5112243189- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Glioma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Rice Cultivation and Yield Improvement
- Ferroptosis and cancer prognosis
- Meningioma and schwannoma management
- Seed Germination and Physiology
- RNA regulation and disease
- MicroRNA in disease regulation
- Plant Genetic and Mutation Studies
- Epigenetics and DNA Methylation
- Innovation and Socioeconomic Development
- Circular RNAs in diseases
- Soybean genetics and cultivation
- Genetic Mapping and Diversity in Plants and Animals
- Plant tissue culture and regeneration
- Spinal Cord Injury Research
- Head and Neck Surgical Oncology
- Trigeminal Neuralgia and Treatments
- Medical Imaging and Analysis
- Cancer Genomics and Diagnostics
- Spinal Fractures and Fixation Techniques
- GABA and Rice Research
University of Maryland, College Park
2021-2025
Linyi People's Hospital
2014-2025
Nanjing Agricultural University
2017-2022
Guangzhou University of Chinese Medicine
2022
Shandong University
2013-2019
Abstract CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic regions. Comprehensive engineering requires simultaneous of multiple genes at defined locations. Here, to expand the scope Cas12a, we screen nine Cas12a orthologs that have not been demonstrated in plants, and identify six, ErCas12a, Lb5Cas12a, BsCas12a, Mb2Cas12a, TsCas12a MbCas12a, possess high activity rice. Among them, Mb2Cas12a stands out with efficiency tolerance low temperature. An engineered...
Seed germination is a complex trait determined by both quantitative loci (QTLs) and environmental factors also their interactions. In this study, we mapped one major QTLqSE3 for seed seedling establishment under salinity stress in rice. To understand the molecular basis of QTL, isolated qSE3 map-based cloning found that it encodes K+ transporter gene, OsHAK21. The expression was significantly upregulated germinating seeds. Physiological analysis suggested increased Na+ uptake seeds stress,...
Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has been widely used for genome editing in mammalian cells and plants due to its distinct characteristics from Cas9. Despite being one of most robust nucleases, LbCas12a general is less efficient than SpCas9 human cells, animals, plants.
Abstract Cytosine base editors (CBEs) and adenine (ABEs) enable precise C-to-T A-to-G edits. Recently, ABE8e, derived from TadA-8e, enhances edits in mammalian cells plants. Interestingly, TadA-8e can also be evolved to confer editing. This study compares engineered CBEs rice tomato cells, identifying TadCBEa, TadCBEd, TadCBEd_V106W as efficient with high purity a narrow editing window. A dual editor, TadDE, promotes simultaneous Multiplexed TadCBEa TadDE is demonstrated transgenic rice, no...
Glioma is the most common primary malignant tumor of central nervous system, and due to limited effectiveness traditional single-target therapies, there an urgent need for new therapeutic targets. Centromere protein F (CENPF) belongs centromere family mainly involved in regulation cell cycle. CENPF has recently been found play a key role tumorigenesis progression, but its gliomas not well studied. The expression level clinical information were obtained by analyzing TCGA, CGGA GEO databases....
Seed vigour is an imperative trait for the direct seeding of rice. Isopropylmalate synthase (IPMS) catalyses committed step leucine (Leu) biosynthesis, but its effect on seed remains unclear. In this study, rice OsIPMS1 and OsIPMS2 was cloned, roles in were mainly investigated. catalyse Leu feedback inhibits their IPMS activities. Disruption resulted low under various conditions, which might be tightly associated with reduction amino acids germinating seeds. Eleven that stress tolerance, GA...
As a precise genome editing technology, base is broadly used in both basic and applied plant research. Cytosine editors (CBEs) adenine (ABEs) represent the two commonly editor types that mediate C-to-T A-to-G transition changes at target sites, respectively. To date, no transversion have been described plants. Here, we assessed three C-to-G (CGBEs) for targeting sequences with SpCas9’s canonical NGG protospacer adjacent motifs (PAMs) as well PAM-less SpRY-based CGBEs relaxed PAM...
Abstract Seed germination plays a pivotal role in the plant life cycle, and its precise regulatory mechanisms are not clear. In this study, 19 quantitative trait loci (QTLs) associated with rice seed were identified through genome-wide association studies (GWAS) of following traits 2016 2017: rate (GR) at 3, 5, 7 days after imbibition (DAI) index (GI). Two major stable QTLs, qSG4 qSG11.1, found to be GR GI over 2 continuous years. Furthermore, OsPK5, encoding pyruvate kinase, was shown...
Glioma is the most prevalent type of adult primary brain tumor and chemotherapy glioma was limited by drug-resistance. Linalool an acyclic monoterpene alcohol possessing various pharmacological activities. The present study conducted to evaluate effect linalool on cell growth. viability in U87-MG cells investigated results showed that significantly reduced a concentration- time-dependent manner. In addition, exposure resulted concentration-dependent increase TUNEL-stained cells, indicating...
Enhancement of salinity tolerance during seed germination is very important for direct seeding in rice. In this study, the salt-tolerant japonica landrace Jiucaiqing was used to determine regulators that are involved imbibition under salt stress. Briefly, comparative proteomic analysis conducted between dry (0 h) and imbibed (24 seeds with 150 mM NaCl. Under stress, uptake water increased rapidly before 24 h (Phase I), followed by a plateau from until 96 II). We identified fourteen proteins...
Summary Cytosine base editors (CBEs) can install a predefined stop codon at the target site, representing more predictable and neater method for creating genetic knockouts without altering genome size. Due to enhanced predictability of editing outcomes, it is also efficient obtain homozygous mutants in first generation. With recent advancement CBEs on improved activity, purify specificity plants animals, has become appealing technology generating knockouts. However, there lack design tools...
Abstract Background Glioma is the most common primary intracranial tumour and has a very poor prognosis. Pyroptosis, also known as inflammatory necrosis, type of programmed cell death that was discovered in recent years. The expression role pyroptosis-related genes gliomas are still unclear. Methods In this study, we analysed RNA-seq clinical information glioma patients from Cancer Genome Atlas (TCGA) database Chinese (CGGA) database. To investigate prognosis immune microenvironment gliomas,...
Abstract Aims Experimental evidence demonstrated a crucial role of TROAP (Trophinin‐associated protein) in regulating the cell proliferation multiple tumors, while expression and function were largely unknown glioma. We aimed to investigate oncogenic its potential mechanisms gliomagenesis. Methods Four gene databases (GEO, TCGA, GTEx CCLE) enrolled our study used for survival analysis. was quantified by qRT‐PCR, western blot immunohistochemistry assays glioma tissues lines. knockdown...
Summary The cultivation of rice varieties with high seed vigour is vital for the direct seeding rice, and molecular basis regulation remains elusive. Here, we cloned a new gene OsHIPL1 , which encodes hedgehog‐interacting protein‐like 1 protein as causal major QTL qSV3 vigour. was mainly localized in plasma membrane nucleus. RNA sequencing (RNA‐seq) revealed that ABA‐related genes were involved rice. higher levels endogenous ABA measured germinating seeds mutants NIL‐ qsv3 line compared to...
Abstract Adenine base editors (ABEs) are valuable, precise genome editing tools in plants. In recent years, the highly promising ADENINE BASE EDITOR8e (ABE8e) was reported for efficient A-to-G editing. However, compared to monocots, comprehensive off-target analyses ABE8e lacking dicots. To determine occurrence of effects tomato (Solanum lycopersicum), we assessed and a high-fidelity version, ABE8e-HF, at 2 independent target sites protoplasts, as well stable T0 lines. Since demonstrated...
CRISPR-Cas-based cytosine base editors (CBEs) are prominent tools that perform site-specific and precise C-to-T conversions catalysed by cytidine deaminases. However, their use is often constrained stringent editing preferences for genomic contexts, off-target effects restricted windows. To expand the repertoire of CBEs, we systematically screened 66 novel deaminases sourced from various organisms, predominantly animal kingdom benchmarked them in rice protoplasts using nCas9-BE3...
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR) systems have been demonstrated to be the foremost compelling genetic tools for manipulating prokaryotic and eukaryotic genomes. Despite robustness versatility of Cas9 Cas12a/b nucleases in mammalian cells plants, their large protein sizes may hinder downstream applications. Therefore, investigating compact CRISPR will unlock numerous genome editing delivery challenges that constrain engineering crop development....