A5088197557- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Plant-Microbe Interactions and Immunity
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant Pathogens and Fungal Diseases
- Genetics and Plant Breeding
- Mycotoxins in Agriculture and Food
- Plant Reproductive Biology
- Plant Virus Research Studies
- Plant pathogens and resistance mechanisms
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- Plant Pathogenic Bacteria Studies
- Plant Gene Expression Analysis
- Genomics and Chromatin Dynamics
- Plant and Fungal Interactions Research
- Phytase and its Applications
- Genomics and Phylogenetic Studies
- Crop Yield and Soil Fertility
- Bioenergy crop production and management
- CRISPR and Genetic Engineering
Nanjing Agricultural University
2016-2025
Centro Internacional de Mejoramiento de Maíz Y Trigo
2018
PLA Navy General Hospital
2018
Henan University
2013
Chinese Academy of Sciences
2011
Institute of Botany
2011
University of California, Davis
2011
University of Wisconsin–Madison
2007-2008
Powdery mildew resistance gene Pm21 , located on the chromosome 6V short arm of Haynaldia villosa and transferred to wheat as a 6VS·6AL translocation (T6VS·6AL), confers durable broad-spectrum powdery mildew. has become key resource for breeding all over world. In China, 12 varieties containing have been planted more than 3.4 million hectares since 2002. intractable molecular genetic mapping because 6VS does not pair recombine with 6AS. Moreover, known accessions H. are immune fungus. is...
Fusarium head blight (FHB), caused mainly by graminearum (Fg) Schwabe (teleomorph: Gibberellazeae Schwble), brings serious damage to wheat production. Chinese landrace Wangshuibai is one of the most important resistance sources in world. The knowledge mechanism underlying its FHB still limited.To get an overview transcriptome characteristics during infection Fg, a high-throughput RNA sequencing based on next generation (NGS) technology (Illumina) were performed. Totally, 165,499 unigenes...
Genomes of varying sizes have been sequenced with next-generation sequencing platforms. However, most reference sequences include draft unordered scaffolds containing chimeras caused by mis-scaffolding. A BioNano genome (BNG) optical map was constructed to improve the previously flax (Linum usitatissimum L., 2n = 30, about 373 Mb), which consisted 3852 larger than 1 kb and totalling 300.6 Mb. The high-resolution BNG cv. CDC Bethune totalled 317 Mb 251 contigs an N50 2.15 total 622 (286.6 Mb,...
Summary Genotype restriction poses a significant bottleneck to stable transformation in the vast majority of plant species, thereby severely impeding advancement bioengineering, particularly for crops. Nanoparticles (NPs) can serve as effective carriers transient delivery nucleic acids, facilitating gene overexpression or silencing plants genotype‐independent manner. However, applications NP‐mediated systems comprehensive genomic studies remained underexplored plants, especially crops that...
Sex chromosomes evolved from autosomes. Recombination suppression in the sex-determining region and accumulation of deleterious mutations lead to degeneration Y many species with heteromorphic X/Y chromosomes. However, how recombination suppressed domain expands locus entire chromosome remains elusive. The papaya ( Carica ) diverged X approximately 2–3 million years ago represents one most recently emerged Here, we report that male-specific (MSY) spans ∼13% chromosome. Interestingly,...
The APETALA 2/Ethylene-responsive element binding factor (AP2/ERF) transcription gene family is widely involved in the biotic and abiotic stress regulation. H. villosa (VV, 2n=14), a wild species of wheat, potential pool for wheat improvement. confers high resistance to several diseases tolerance some stress. In this study, ERF1-V, an ethylene-responsive element-binding AP2/ERF from villosa, was cloned characterized. Sequence phylogenetic analysis showed that ERF1-V deduced B2 type ERF gene....
Pasmo is one of the most widespread diseases threatening flax production. To identify genetic regions associated with pasmo resistance (PR), a genome-wide association study was performed on 370 accessions from core collection. Evaluation severity in field 2012 to 2016 Morden, MB, Canada. Genotyping-by-sequencing has identified 258,873 single nucleotide polymorphisms (SNPs) distributed all 15 chromosomes. Marker-trait associations were using ten different statistical models. A total 692...
Plant sense potential microbial pathogen using pattern recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). The Lectin receptor-like kinase genes (LecRKs) are involved in various cellular processes mediated by signal transduction pathways. In the present study, an L-type lectin receptor gene LecRK-V was cloned from Haynaldia villosa, a diploid wheat relative which is highly resistant powdery mildew. expression of rapidly up-regulated Bgt inoculation and...
Pasmo (Septoria linicola) is a fungal disease causing major losses in seed yield and quality stem fibre flax. resistance (PR) quantitative has low heritability. To improve PR breeding efficiency, the accuracy of genomic prediction (GP) was evaluated using diverse worldwide core collection 370 accessions. Four marker sets, including three defined by 500, 134 67 previously identified trait loci (QTL) one 52,347 PR-correlated genome-wide single nucleotide polymorphisms, were used to build ridge...
Heavy-metal-associated (HMA) isoprenylated plant proteins (HIPPs) only exist in vascular plants. They play important roles responses to biotic/abiotic stresses, heavy-metal homeostasis, and detoxification. However, research on the distribution, diversification, function of HIPPs Triticeae species is limited. In this study, a total 278 were identified from database five species, 13 cloned Haynaldia villosa. These genes classified into groups by phylogenetic analysis. Most had one HMA domain,...
Abstract I-motifs (iMs) are non-canonical DNA secondary structures that fold from cytosine (C)-rich genomic regions termed putative i-motif forming sequences (PiMFSs). The structure of iMs is stabilized by hemiprotonated C-C base pairs, and their functions now suspected in key cellular processes human cells such as genome stability regulation gene transcription. In plants, biological relevance still largely unknown. Here, we characterized PiMFSs with high potential for formation the rice...
Bread wheat (Triticum aestivum) is an allohexaploid that generated by two subsequent allopolyploidization events. The large genome size (16 Gb) and polyploid complexity impede our understanding of how regulatory elements their interactions shape chromatin structure gene expression in wheat. open enrichment network Hi-C (OCEAN-C) a powerful antibody-independent method to detect between regions throughout the genome.
Dehydration-responsive element-binding (DREB) transcription factor (TF) plays a key role for abiotic stress tolerance in plants. In this study, novel cDNA encoding DREB factor, designated SsDREB , was isolated from succulent halophyte Suaeda salsa . This protein classified the A-6 group of subfamily based on multiple sequence alignments and phylogenetic characterization. Yeast one-hybrid assays showed that specifically binds to DRE could activate expression reporter genes yeast, suggesting...
Summary Powdery mildew is one of the most devastating wheat fungal diseases. A diploid relative, Haynaldia villosa L., highly resistant to powdery mildew, and its genetic resource resistances, such as Pm21 locus, now widely used in breeding. Here we report cloning a resistance gene from H. , designated CMPG 1–V that encodes U–box E3 ubiquitin ligase. Expression was induced leaf stem upon inoculation with Blumeria graminis f. sp. tritici ( Bgt ) fungus, presence essential for rapid induction...
We conducted genome-wide identification of R-loops followed by integrative analyses with relation to gene expression and epigenetic signatures in the rice genome. found that correlation between levels profiled R-loop peak was dependent on positions within structures (hereafter named “genic position”). Both antisense only (ASO)-R-loops sense/antisense (S/AS)-R-loops sharply peaked around transcription start sites (TSSs), these corresponded positively transcript overlapping genes. In contrast,...
Wheat (Triticum aestivum) is among the most important staple crops for safeguarding food security of growing world population. To bridge gap between genebank diversity and breeding programs, we developed an advanced backcross-nested association mapping plus inter-crossed population (AB-NAMIC) by crossing three popular wheat cultivars as recurrent founders to 20 germplasm lines from a mini core collection. Selective backcrossing combined with selection against undesirable traits extensive...
SERRATE (SE) is a core protein for microRNA (miRNA) biogenesis as well mRNA alternative splicing. Investigating the regulatory mechanism of SE expression hence critical to understanding its detailed function in diverse biological processes. However, little about control has been clarified, especially through long noncoding RNA (lncRNA). Here, we identified an antisense intragenic lncRNA transcribed from 3' end SE, named SEAIRa. SEAIRa repressed expression, which turn led serrated leaves....