To investigate the prevalence and genetic environment of multiresistance gene oqxAB in Salmonella enterica serotype Typhimurium isolated from food-producing animals. In this study, 63 isolates were analysed for presence plasmid-mediated quinolone resistance determinants mutations resistance-determining region by molecular methods (PCR/sequencing). The oqxAB-positive typed pulsed-field gel electrophoresis (PFGE). Plasmids carrying studied conjugation/transformation, replicon typing, Southern...

Bread wheat (Triticum aestivum) is an allohexaploid that generated by two subsequent allopolyploidization events. The large genome size (16 Gb) and polyploid complexity impede our understanding of how regulatory elements their interactions shape chromatin structure gene expression in wheat. open enrichment network Hi-C (OCEAN-C) a powerful antibody-independent method to detect between regions throughout the genome.

Salmonella spp. are one of the most important foodborne bacterial pathogens in human beings and animals. This study aimed to analyze prevalence characterization from broilers Shandong, China. A total 67 were recovered 600 rectal swabs collected 3 large-scale intensive broiler farms (67/600, 11.2%) between May October 2018. Among isolates, common serovars S. enteritidis typhimurium. The highest occurrence resistance observed was for polymyxin (100%), followed by ampicillin (68.7%)....

Summary Plant with naturally twisted branches is referred to as a tortuous‐branch plant, which have extremely high ornamental value due their zigzag shape and the natural twisting of branches. Prunus mume an important woody plant. However, molecular mechanism underlying this unique trait in genus unknown. Here, we present chromosome‐level genome assembly cultivated P. var. tortuosa created using Oxford Nanopore combined Hi‐C scaffolding, resulted 237.8 Mb being anchored onto eight...

Abstract Low temperature is one of the most important abiotic factors limiting growth, development and geographical distribution plants. Prunus mume an attractive woody ornamental plant that blooms in early spring Beijing. However, molecular mechanisms underlying cold hardening to enhance freezing tolerance genus remains elusive. This study examined dynamic physiological responses induced by hardening, identified freezing-tolerance genes RNA-seq ATAC-seq analyses. Cold elevated content...

Prunus mume is an important woody plant that has high ornamental and economic value, widely distributed used in landscape architecture East Asia. In plants, basic (region) leucine zipper (bZIP) transcription factors play regulatory roles growth, development, dormancy abiotic stress. To date, bZIP have not been systematically studied P. mume. this study, 49 genes were first identified mume, the PmbZIP family was divided into 12 groups according to grouping principles for Arabidopsis thaliana...

Plant architecture includes vital traits that influence and benefit crops, economically important trees. Different plant architectures provide natural beauty. Weeping ornamental plants are aesthetically appealing to people. The regulatory mechanism controlling the weeping trait is poorly understood in crape myrtle. To investigate mechanism, transcriptional profiling of different organs upright myrtle was performed based on phenotype. Phenotypic histological analyses demonstrated endodermal...

Prunus mume is an important ornamental woody plant that grows in tropical and subtropical regions. Freezing stress can adversely impact productivity limit the expansion of geographical locations. Understanding cold-responsive genes could potentially bring about development new ways to enhance freezing tolerance. Members serine/threonine protein kinase ( CIPK ) gene family play roles abiotic stress. However, function P. remains poorly defined. A total 16 were first identified . systematic...

Rosaceae is an important family containing some of the highly evolved fruit and ornamental plants. Abiotic stress responses play key roles in seasonal growth development However, molecular basis remains largely unknown Rosaceae. Abscisic acid (ABA) a hormone involving abiotic response pathways. The ABRE-binding factor/ABA-responsive element-binding protein (ABF/AREB) subfamily basic domain/leucine zipper (bZIP) transcription factor family. It plays role ABA-mediated signaling pathway. Here,...

Waterfowl have a high likelihood of being infected with Riemerella anatipestifer. Although the pathogen is found in domestic ducks, turkeys, geese, and wild birds, there little information available about consequences infection during egg laying hatching chickens. Here, we present first report novel sequence type R. anatipestifer S63 isolated from chickens China. On basis pan-genome analysis, showed S63's genome occupies distinct branch other isolates hosts. Galleria mellonella larval tests...

Plant dormancy is essential for perennial plant survival. Different genotypes of Prunus mume, including Eumume group and Apricot Mei group, undergo leaf senescence at different times. In order to verify the cold resistance P. freeze evaluation was carried out. Our results showed that had a stronger freezing tolerance than occurred an earlier period before winter. Based on phenotypic data in response seasonal climate change, significant candidate regions were selected using GWAS. Furthermore,...

MYBs (v-myb avian myeloblastosis viral oncogene homologs) are important transcriptional regulators that play critical roles in the regulation of anthocyanin biosynthesis. The overexpression MYB genes has been reported different plant species. However, inconsistent strategies to assess transgenic plants have made it difficult explain complex mechanisms biosynthesis by MYBs. We report here a meta-analysis 608 studies from 206 publications assessing effects on anthocyanins and evaluate...

Trehalose and its key synthase (trehalose-6-phosphate synthase, TPS) can improve the drought tolerance of plants. However, little is known about roles trehalose TPS family in Prunus mume response to drought. In our study, we discovered that content leaf, root, stem tissues significantly increased P. Therefore, characteristics functions are worth investigating mume. We identified nine members mume, which were divided into two sub-families characterized by gene structure, promoter elements,...

Tea, the world’s second most traded commodity, significantly impacts economies of producing countries. However, prolonged cultivation leads to soil degradation, particularly through acidification and depletion essential nutrients, which adversely affects tea quality. This study investigates effects biomass materials—biochar, organic fertilizer, bio-organic fertilizer—on both quality properties. The results revealed that all treatments improved catechin index (CQI) tea, with fertilizer (BOF)...

Abstract Prunus mume (also known as Mei) is an important ornamental plant that popular with Asians. The weeping trait in P. has attracted the attention of researchers for its high value. However, formation woody plants a complex process and molecular basis stem development unclear. Here, morphological histochemical characteristics transcriptome profiles upright stems from were studied. Significant alterations observed, absence phloem fibres less xylem might be responsible their inability to...

VIGS (Virus-induced gene silencing), a method for posttranscriptional silencing, is an effective technique investigating the activities of genes in plants. Since there no report available system Styrax japonicus, application approach that results knockdown to study function limited. In this study, we compared characteristics could affect viability S. including acetosyringone (AS) concentration, Agrobacterium’s optical density and inoculation method. The stable reference japonicus were...

Cyclins, a prominent class of cell division regulators, play an extremely important role in plant growth and development. D-type cyclins (CYCDs) are the rate-limiting components G1 phase. In plants, studies CYCDs mainly concerned with herbaceous yet little information is available about these genes perennial woody especially ornamental plants. Here, twelve Prunus mume CYCD (PmCYCDs) identified characterized. The PmCYCDs were named on basis orthologues Arabidopsis thaliana Oryza sativa. Gene...

Development of antibiotic resistance may alter the virulence properties bacterial organisms. In this study, nine clinical ceftriaxone-susceptible Salmonella enterica serotype Typhimurium strains were subjected to stepwise selection with increasing concentrations ceftriaxone in culture media. Mutations virulence-associated genes and efflux analyzed by polymerase chain reaction (PCR) DNA sequencing. The expression levels invA stn as well pump tolC, arcA, arcB before after measured real-time...

Streptococcus suis remains a serious threat to the worldwide swine industry and human health. In this study, rapid assays for detection of three common virulence-related factors (mrp, epf, sly) were developed, evaluated, applied. Loop-mediated isothermal amplification (LAMP) primers designed using Primer Explorer V5 software. The sensitivity specificity LAMP determined based on sample turbidity. For all genes, performed at 62°C with reaction time 60 min. limit conventional polymerase chain...

Abstract Background Salmonella spp. are one of the most important foodborne bacterial pathogens in human beings and animals. The prevalence from broilers Shandong, China antimicrobial susceptibility these isolates was determined. Results From May to October 2018, 600 samples collected, 67 were recovered with an isolation rate 11.2%. common serovars S. enteritidis typhimurium. highest incidence resistance observed for PB (100%), AMP (68.7%), MDR isolate 53.7%. Four β-lactamase genes detected...

ABSTRACT Escherichia coli is a pathogenic bacterium that widely distributed and can lead to serious illnesses in both humans animals. As there rising incidence of multidrug resistance among these bacteria, it has become imperative discover alternative therapies beyond antibiotics effectively treat such infections. Bacteriophage (phage) therapy the potential infections caused by E. , as phages contain enzymes cause lysis or destruction bacterial cells. Simultaneously, easy accessibility...