Longfei Wang

ORCID: 0000-0003-3763-7833
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About
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Research Areas
  • Chromosomal and Genetic Variations
  • Plant tissue culture and regeneration
  • Plant Molecular Biology Research
  • Plant Reproductive Biology
  • Soybean genetics and cultivation
  • Wheat and Barley Genetics and Pathology
  • Plant nutrient uptake and metabolism
  • Legume Nitrogen Fixing Symbiosis
  • Plant Virus Research Studies
  • Plant Genetic and Mutation Studies
  • Genetic Mapping and Diversity in Plants and Animals
  • Cytomegalovirus and herpesvirus research
  • Genetic Syndromes and Imprinting
  • interferon and immune responses
  • Genetics and Plant Breeding
  • Plant Stress Responses and Tolerance
  • Biochemical and Molecular Research
  • Hydrogels: synthesis, properties, applications
  • Crop Yield and Soil Fertility
  • Plant responses to water stress
  • Collagen: Extraction and Characterization
  • Genomics and Chromatin Dynamics
  • Polysaccharides and Plant Cell Walls
  • Soil Mechanics and Vehicle Dynamics
  • Rice Cultivation and Yield Improvement

Nanjing Agricultural University
2017-2025

China University of Geosciences
2025

Taiyuan University of Technology
2023

Second Affiliated Hospital of Jilin University
2022

Wuhan University
2022

Yangzhou University
2020

Chinese Academy of Fishery Sciences
2015

Qingdao National Laboratory for Marine Science and Technology
2015

National University of Singapore
2014

High-resolution genetic maps are essential for fine mapping of complex traits, genome assembly, and comparative genomic analysis. Single-nucleotide polymorphisms (SNPs) the primary molecular markers used map construction. In this study, we identified 13,362 SNPs evenly distributed across Japanese flounder (Paralichthys olivaceus) genome. Of these SNPs, 12,712 high-confidence were subjected to high-throughput genotyping assigned 24 consensus linkage groups (LGs). The total length was 3,497.29...

10.1093/dnares/dsv001 article EN cc-by-nc DNA Research 2015-03-11

Polyploidy is a prominent feature for genome evolution in many animals and all flowering plants. Plant polyploids often show enhanced fitness diverse extreme environments, but the molecular basis this remains elusive. Soil salinity presents challenges plants including agricultural crops. Here we report that salt tolerance tetraploid rice through lower sodium uptake correlates with epigenetic regulation of jasmonic acid (JA)-related genes. induces DNA hypomethylation potentiates genomic loci...

10.1073/pnas.2023981118 article EN Proceedings of the National Academy of Sciences 2021-03-26

Abstract Polyploidy or whole-genome duplication (WGD) is widespread in plants and a key driver of evolution speciation, accompanied by rapid dynamic changes genomic structure gene expression. The 3D the genome intricately linked to expression, but its role transcription regulation following polyploidy domestication remains unclear. Here, we generated high-resolution (∼2 kb) Hi-C maps for cultivated soybean (Glycine max), wild soja), common bean (Phaseolus vulgaris). We found polyploidization...

10.1093/plcell/koab081 article EN The Plant Cell 2021-03-13

Genetic imprinting refers to the unequal expression of paternal and maternal alleles a gene in sexually reproducing organisms, including mammals flowering plants. Although many imprinted genes have been identified plants, functions these remained largely uninvestigated. We report genome-wide analysis expression, DNA methylation small RNAs rice endosperm functional tests five during seed development using Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated gene9...

10.1111/nph.14510 article EN publisher-specific-oa New Phytologist 2017-03-13

Bread wheat (Triticum aestivum) is an allohexaploid that generated by two subsequent allopolyploidization events. The large genome size (16 Gb) and polyploid complexity impede our understanding of how regulatory elements their interactions shape chromatin structure gene expression in wheat. open enrichment network Hi-C (OCEAN-C) a powerful antibody-independent method to detect between regions throughout the genome.

10.1186/s13059-022-02611-3 article EN cc-by Genome biology 2022-01-24

Abstract Background Hybridization and backcrossing are commonly used in animal plant breeding to induce heritable variation including epigenetic changes such as paramutation. However, the molecular basis for hybrid-induced memory remains elusive. Results Here, we report that hybridization between inbred parents B73 Mo17 induces trans -acting hypermethylation hypomethylation at thousands of loci; several hundreds (~ 3%) transmitted through six three selfing generations. Notably, many...

10.1186/s13059-022-02614-0 article EN cc-by Genome biology 2022-02-09

Between the two major rice subspecies, indica varieties generally exhibit higher nitrate (NO3‒) uptake and nitrogen (N)-use efficiency (NUE) than japonica varieties. Introducing efficient NO3‒ utilization alleles from into could improve NUE, at same time uncover unknown regulators of metabolism. Here, we identify OsWRKY23 as a key regulator NUE differences between rice. The OsWRKY23indica allele exhibits reduced transcriptional activation negative auxin accumulation, DULL NITROGEN RESPONSE1...

10.1038/s41467-025-56752-7 article EN cc-by-nc-nd Nature Communications 2025-02-06

The tip of the root is covered by a thimble-shaped cap that site perception and transduction for many environmental stimuli. Until now, little was known about how rice (Oryza sativa) develops functions to regulate adaptive behavior root. To address this, we examined formation during embryogenesis characterized anatomy structure radicle cap. We further investigated role quiescent center in de novo origin At molecular level, found shoot-derived auxin absolutely needed trigger regeneration when...

10.1104/pp.114.240929 article EN PLANT PHYSIOLOGY 2014-06-23

Soybean (Glycine max L. Merrill) is one of the most important commercial crops worldwide. However, soybean has undergone severe genetic bottlenecks during domestication (Hyten et al., 2006). It essential to exploit novel sources diversity and expand gene pools for improvement. Plant mutation breeding been widely used by plant breeders create diversity. Ethyl methanesulfonate (EMS) a chemical mutagen believed mainly induce point mutations, which commonly develop mutant populations in (Li...

10.1111/pbi.13844 article EN Plant Biotechnology Journal 2022-05-17

Abstract Rational plant density and fertilizer management are important practices to improve maize ( Zea mays L.) yield nutrient use efficiency. Previous research indicated that fertilization had positive interaction on N utilization. The studied the interactive effects of slow‐released (added amino acid polymer biological preparation) nitrogen efficiencies (NUEs). A 2‐yr field experiment with varieties Suyu29 (SY29) or Suyu30 (SY30) was conducted using a split‐split plot design. Three...

10.1002/agj2.20075 article EN Agronomy Journal 2020-01-01

The cyclic oligonucleotide-based anti-phage signaling system (CBASS) is a family of defense in prokaryotes 1, 2 . Composed GMP-AMP synthase (cGAS) and CBASS-associated proteins, CBASS utilizes oligonucleotides to activate antiviral immunity 3–6 One major group proteins are homologs eukaryotic E2 ubiquitin-conjugating enzymes. However, the function remains elusive. Here, we report that bacterial enzyme regulates cGAS by imitating entire ubiquitination cascade. This includes processing...

10.1101/2022.05.25.493364 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2022-05-25

Transcription factors (TFs) bind regulatory genomic regions to orchestrate spatio-temporal expression of target genes. Global dissection the cistrome is critical for elucidating transcriptional networks underlying complex agronomic traits in crops. Here, we generate a comprehensive genome-wide binding map 148 TFs using DNA affinity purification sequencing soybean. We find TF sites (TFBSs) exhibit elevated chromatin accessibility and contain more rare alleles than other regions. Intriguingly,...

10.1186/s13059-024-03454-w article EN cc-by-nc-nd Genome biology 2024-12-18

The development of precision medicine in 21st century has accelerated a new area medicine, bringing precise project diagnosis and treatment.The techniques for genetic diseases including next generation sequencing copy number variation detection technology have facilitated the treatment rare children's neural system, providing directed therapies especially creatine deficiency syndromes, pyridoxine dependent epilepsy, glucose transporter type 1 syndrome, malignant migrating partial seizures...

10.3760/cma.j.issn.2095-428x.2016.12.021 article EN Zhonghua shiyong erke linchuang zazhi 2016-06-20

Abstract The cyclic oligonucleotide based anti phage signaling systems (CBASSs) are a family of defense in prokaryotes. Composed GMP-AMP synthase (cGAS) and CBASS-associated proteins, CBASSs utilize oligonucleotides to activate antiviral immunity. One major group proteins homologs eukaryotic E2 ubiquitin-conjugating enzymes. However, the function remains elusive. Here, we report that bacterial forms covalent bond with cGAS, between active site glycine near C-terminus cGAS. Unlike...

10.21203/rs.3.rs-1706142/v1 preprint EN cc-by Research Square (Research Square) 2022-06-10
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