A5004444258- Wheat and Barley Genetics and Pathology
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Disease Resistance and Genetics
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- DNA and Nucleic Acid Chemistry
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Genetic Mapping and Diversity in Plants and Animals
- Lipid metabolism and biosynthesis
- Plant Virus Research Studies
- Rice Cultivation and Yield Improvement
- Soybean genetics and cultivation
- EFL/ESL Teaching and Learning
- Genomics and Chromatin Dynamics
- Higher Education and Teaching Methods
- Plant Micronutrient Interactions and Effects
- Endoplasmic Reticulum Stress and Disease
- Photosynthetic Processes and Mechanisms
- Second Language Learning and Teaching
- RNA and protein synthesis mechanisms
Nanjing Agricultural University
2006-2025
First Affiliated Hospital of Henan University of Science and Technology
2024
Zhengzhou Children's Hospital
2024
Zhengzhou University
2024
Henan Institute of Science and Technology
2022
Sichuan Agricultural University
2012
Rockefeller University
1988
University of Oxford
1988
Salt stress impairs nutrient metabolism in plant cells, leading to growth and yield penalties. However, the mechanism by which plants alter their processes response salt remains elusive. In this study, we identified characterized rice (Oryza sativa) tolerant 1 (rst1) mutant, displayed improved tolerance grain yield. Map-based cloning revealed that gene RST1 encoded an auxin factor (OsARF18). Molecular analyses showed directly repressed expression of encoding asparagine synthetase (OsAS1)....
Abstract Chloride (Cl − ) ions cause major damage to crops in saline soils. Understanding the key factors that influence Cl uptake and translocation will aid breeding of more salt-tolerant crops. Here, using genome-wide association study transcriptomic analysis, we identified a NITRATE TRANSPORTER 1 (NRT1)/PEPTIDE family (NPF) protein, GmNPF7.5, as dominant gene locus influencing homeostasis soybean (Glycine max). A natural SNP variation resulted two haplotypes (GmNPF7.5 HapA GmNPF7.5 HapB...
ABSTRACT We describe a method for permeabilizing and extracting cells that preserves both structure function whilst allowing the cell derivatives to be handled freely. Cells are encapsulated in microbeads of agarose; coat agarose, which is freely permeable small molecules, forms protective layer around fragile constituents. then permeabilized by non-ionic detergent Triton X-100 or antibody complement buffer whose ionic composition mimics cytoplasm. The resulting structures have been...
Genetic imprinting refers to the unequal expression of paternal and maternal alleles a gene in sexually reproducing organisms, including mammals flowering plants. Although many imprinted genes have been identified plants, functions these remained largely uninvestigated. We report genome-wide analysis expression, DNA methylation small RNAs rice endosperm functional tests five during seed development using Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated gene9...
Bread wheat (Triticum aestivum) is an allohexaploid that generated by two subsequent allopolyploidization events. The large genome size (16 Gb) and polyploid complexity impede our understanding of how regulatory elements their interactions shape chromatin structure gene expression in wheat. open enrichment network Hi-C (OCEAN-C) a powerful antibody-independent method to detect between regions throughout the genome.
Due to the potential off-tumor signal leakage and limited biomarker content, there is an urgent need for stimulus-responsive amplification-based tumor molecular imaging strategies. Therefore, two tetrahedral framework DNA (tFNA-Hs), tFNA-H1AP, tFNA-H2, were rationally engineered form a polymeric tFNA network, termed intelligent in AND-gated manner. The network was designed tumor-specific by leveraging elevated expression of apurinic/apyrimidinic endonuclease 1 (APE1) cytoplasm instead normal...
The dynamic changes in membrane phospholipids affect biophysical properties and cell signaling, thereby influencing numerous biological processes. Nonspecific phospholipase C (NPC) enzymes hydrolyze common to release diacylglycerol (DAG), which is converted phosphatidic acid (PA) other lipids. In this study, 2 Arabidopsis (Arabidopsis thaliana) tandemly arrayed genes, NPC3 NPC4, were identified as critical factors modulating auxin-controlled plant growth tropic responses. Moreover, NPC4...
Summary Polyploidy occurs in some animals and all flowering plants, including important crops such as wheat. The consequences of polyploidy remain elusive, partly because their progenitors are unknown. Using two resynthesized wheat allotetraploids S l AA AADD with known diploid progenitors, we analyzed mRNA small RNA transcriptomes the endosperm, compared between endosperm root , examined chromatin changes allotetraploids. In there were more non‐additively expressed genes than ....
Abstract Background Wheat is a powerful genetic model for studying polyploid evolution and crop domestication. Hexaploid bread wheat was formed by two rounds of interspecific hybridization polyploidization, processes which are often accompanied epigenetic changes, including DNA methylation. However, the extent effect such changes during evolution, particularly from tetraploid-to-hexaploid wheat, currently elusive. Results Here we report genome-wide methylation landscapes in extracted...
Bread wheat (Triticum aestivum) is an allohexaploid that was formed via two allopolyploidization events. Growing evidence suggests histone modifications are involved in the response to 'genomic shock' and environmental adaptation during polyploid formation evolution. However, role of modifications, especially H3 lysine-27 dimethylation (H3K27me2), genome evolution remains elusive. Here we analyzed H3K27me2 H3K27me3 profiles hexaploid its tetraploid diploid relatives. Although levels were...
Abstract Ammonium (NH4+) is a key inorganic nitrogen source in cellular amino acid biosynthesis. The coupling of transcriptional and posttranslational regulation AMMONIUM TRANSPORTER (AMT) ensures that NH4+ acquisition by plant roots properly balanced, which allows for rapid adaptation to variety conditions. Here, we report phospholipase D (PLD)-derived phosphatidic (PA) interacts with AMT1;1 mediate uptake Arabidopsis (Arabidopsis thaliana). We examined pldα1 pldδ-knockout mutants found...
The plant IQD gene family is responsive to a variety of stresses. In this study, we studied the structural features and functions BrIQD35 in Chinese cabbage, member family. was cloned shown contain an IQ motif. Transient expression indicated that it localized on plasma membrane significantly upregulated under drought salt stress cabbage. To further identify function BrIQD35, heterologously overexpressed Nicotiana benthamiana. Although there no significant difference between...
Abstract Lipid droplet (LD) biogenesis from the endoplasmic reticulum (ER) is accompanied by conversion of phospholipids to triacylglycerol and expansion phospholipid monolayer surface. The determinants this process are largely unknown in plants. Here, we demonstrate that Arabidopsis thaliana lysophosphatidic acid acyltransferase, LPAT2, its product phosphatidic (PA), critical lipid-based cues for LD proliferation. RNAi suppression LPAT2 decrease PA accumulation at surface LDs, consequently...
SUMMARY Polyploidy is a prominent driver of plant diversification, accompanied with dramatic chromosomal rearrangement and epigenetic changes that affect gene expression. How chromatin interactions within between subgenomes adapt to ploidy transition remains poorly understood. We generate open interaction maps for natural hexaploid wheat (AABBDD), extracted tetraploid (AABB), diploid progenitor Aegilops tauschii (DD) resynthesized (RHW, AABBDD). Thousands intra‐ interchromosomal loops are de...