Phytohormone ethylene controls diverse developmental and physiological processes such as fruit ripening via modulation of signaling pathway. Our previous study identified that ETHYLENE RESPONSE FACTOR11 (MaERF11), a transcription factor in the pathway, negatively regulates banana, but mechanism for MaERF11-mediated transcriptional regulation remains largely unknown. Here we showed MaERF11 has intrinsic repression activity planta. Electrophoretic mobility shift assay chromatin...

ABSTRACT MYC2, a basic helix‐loop‐helix (bHLH) transcription factor, is key regulator in the activation of jasmonate (JA) response. However, molecular details MYC2 involving methyl (MeJA)‐induced chilling tolerance fruit remain largely unclear. In present work, two genes, MaMYC2a and MaMYC2b , one homolog inducer C‐repeat‐binding factor (CBF) gene, MaICE1 were isolated characterized from banana fruit. MaMYC2s found to be all localized nucleus. addition, proline‐rich domain (PRD) acidic (AD)...

Abstract Melatonin and abscisic acid (ABA) play contrasting roles in regulating leaf senescence plants. The molecular mechanism underlying the interaction between melatonin ABA involved senescence, however, remains poorly defined. Herein, we found that exogenous application of delayed Chinese flowering cabbage, accompanied by reduced expression chlorophyll catabolic biosynthetic genes, a lower endogenous level. Significantly, three nucleus‐localized transcriptional activators BrABF1, BrABF4,...

Summary Flavor‐associated volatile chemicals make major contributions to consumers’ perception of fruits. Although great progress has been made in establishing the metabolic pathways associated with synthesis, much less is known about regulation those pathways. Knowledge how are regulated would greatly facilitate efforts improve flavor. Volatile esters contributors fruity flavor notes many species, providing a good model investigate synthesis Here we initiated study peach ( Prunus persica L....

The plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play important roles in plant growth, development, stress responses. However, the precise role of TFs relation to fruit ripening is poorly understood. In this study, six genes, designated MaNAC1-MaNAC6, were isolated characterized from banana fruit. Subcellular localization showed that MaNAC1-MaNAC5 proteins localized preferentially nucleus, while MaNAC6 was distributed throughout entire cell. A transactivation assay...

Although starch degradation has been well studied in model systems such as Arabidopsis leaves and cereal seeds, this process starchy fruits during ripening, especially bananas, is largely unknown. In study, 38 genes encoding degradation-related proteins were identified characterized from banana fruit. Expression analysis revealed that 27 candidate significantly induced fruit with concomitant conversion of starch-to-sugars. Furthermore, iTRAQ-based proteomics experiments 18...

Starch degradation is a necessary process determining banana fruit quality during ripening. Many starch degradation-related genes are well studied. However, the transcriptional regulation of ripening remains poorly understood. In this study, we identified MYB transcription factor (TF) termed MaMYB3, as putative protein binding promoter MaGWD1, member glucan water dikinase (GWD) family which has been demonstrated an important enzyme degradation. MaMYB3 was ripening- and ethylene-repressible,...

Our previous studies have indicated that the banana ripening-induced MaNAC1, a NAC (NAM, ATAF1/2 and CUC2) transcription factor (TF) gene, is regulated by ethylene during fruit ripening, propylene, functional analogue, induces cold tolerance of fruits. However, involvement MaNAC1 in propylene-induced fruits not understood. In present work, possible was investigated. noticeably induced stress or following propylene treatment storage. Transient protoplast assays showed promoter activated...

The DNA binding with one finger (Dof) proteins, a family of plant-specific transcription factors, are involved in variety plant biological processes. However, little information is available on their involvement fruit ripening. We have characterized 25 MaDof genes from banana (Musa acuminata), designated as MaDof1-MaDof25 Gene expression analysis subjected to different ripening conditions revealed that MaDofs were differentially expressed during stages MaDof10, 23, 24, and ethylene-inducible...

Phytohormone abscisic acid (ABA) and plant-specific WRKY transcription factors (TFs) have been implicated to play important roles in various stress responses. The involvement of TFs ABA-mediated cold tolerance economical fruits, such as banana fruit, however remains largely unknown. Here, we reported that ABA application could induce expressions biosynthesis-related genes MaNCED1 MaNCED2, increase endogenous contents, thereby enhance fruit. Four fruit TFs, designated MaWRKY31, MaWRKY33,...

Ethylene plays a critical regulatory role in climacteric fruit ripening, and its biosynthesis is fine-tuned at the transcriptional posttranslational levels. Nevertheless, mechanistic link between regulation of ethylene during ripening largely unknown. This study uncovers coordinated mechanism controlling banana (Musa acuminata) ripening. NAC (NAM, ATAF, CUC) proteins MaNAC1 MaNAC2 repress expression MaERF11, protein previously known to negatively regulate genes MaACS1 MaACO1. A RING E3...

Abstract Most harvested fruits and vegetables are stored at low temperature but many of them highly sensitive to chilling injury. Jasmonic acid (JA), a plant hormone associated with various stress responses, is known reduce injury in fruits. However, little about the transcriptional regulation JA biosynthesis relation cold response Here, we show involvement Group I WRKY transcription factor (TF) from banana fruit, MaWRKY26, regulating biosynthesis. MaWRKY26 was found be nuclear-localized...

Papaya is an important tropical fruit with a rich source of carotenoids. The ripening papaya physiological and metabolic process remarkable changes including accumulation carotenoids, which depends primarily on the action ethylene. Ethylene response mediated by transcriptional cascade involving transcription factor families EIN3/EILs ERFs. Although ERF members have been reported to control carotenoid production in Arabidopsis tomato, whether are also involved biosynthesis during remains...

Papaya fruits accumulate carotenoids during fruit ripening. Although many papaya carotenoid biosynthesis pathway genes have been identified, the transcriptional regulators of these not characterized. In this study, a NAC transcription factor, designated as CpNAC1, was characterized from fruit. CpNAC1 localized exclusively in nucleus and possessed activation activity. Expression phytoene desaturases (CpPDSs) increased ripening by propylene treatment, which correlates well with elevated...

Abstract Horticultural commodities suffer chilling injury following exposure to extremely low temperatures, which results in visible symptoms and considerable quality loss. Therefore, it is of significance understand the mechanism this physiological disorder develop effective strategies control it. Chilling stress causes alteration structure function plasma membrane, assumed be primary event response cold stress. During process, membrane lipid metabolism plays a pivotal role fluidity...

Summary Fruit ripening is a critical phase in the production and marketing of fruits. Previous studies have indicated that fruit highly coordinated process, mainly regulated at transcriptional level, which transcription factors play essential roles. Thus, identifying key regulating as well their associated regulatory networks promises to contribute better understanding ripening. In this study, temporal gene expression analyses were performed investigate banana with aim discern global...

Abstract Banana (Musa acuminata) fruits ripening at 30 °C or above fail to develop yellow peels; this phenomenon, called green ripening, greatly reduces their marketability. The regulatory mechanism underpinning high temperature-induced remains unknown. Here we decoded a transcriptional and post-translational module that causes in banana. showed reduced expression of 5 chlorophyll catabolic genes (CCGs), MaNYC1 (NONYELLOW COLORING 1), MaPPH (PHEOPHYTINASE), MaTIC55 (TRANSLOCON AT THE INNER...

Summary Chilling injury has a negative impact on the quantity and quality of crops, especially subtropical tropical plants. The plant cell wall is not only main source biomass production, but also first barrier to various stresses. Therefore, improving understanding alterations in architecture great significance for both production stress adaptation. Herein, we demonstrated that principal component cellulose accumulated during chilling stress, which was caused by activation MaCESA proteins....

Guavas are typical tropical fruit with high nutritional and commercial value. Because of their thin skin metabolic rate, guavas highly susceptible to water loss, physical damage, spoilage, severely limiting shelf-life. can typically only be stored for approximately one week at room temperature, making transportation, storage, handling difficult, resulting in low profit margins the industry. This review focuses on physiological biochemical changes molecular mechanisms which occur postharvest...

Plants respond to pathogen attack by the modulation of a large set genes, which are regulated different types transcription factor (TF). NAC (NAM/ATAF/CUC) and WRKY plant-specific families TFs, have received much attention as transcriptional regulators in plant defence. However, cooperation between TFs disease response remains largely unknown. Our previous study has revealed that two banana fruit MaWRKY1 MaWRKY2, involved salicylic acid (SA)- methyl jasmonate (MeJA)-induced resistance...

Histone deacetylation plays an important role in epigenetic control of gene expression. HD2 is a plant-specific histone deacetylase that able to mediate transcriptional repression many biological processes. To investigate the and mechanisms longan fruit senescence, one 2-like gene, DlHD2, two ethylene-responsive factor-like genes, DlERF1 DlERF2, were cloned characterized from fruit. Expression these genes was examined during senescence under different storage conditions. The accumulation...