A5043106360- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Crop Yield and Soil Fertility
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Cancer-related gene regulation
- Advanced Battery Materials and Technologies
- Plant Stress Responses and Tolerance
- Advancements in Battery Materials
- RNA modifications and cancer
- Landslides and related hazards
- Plant pathogens and resistance mechanisms
- Viral gastroenteritis research and epidemiology
- Plant-Microbe Interactions and Immunity
- Animal Virus Infections Studies
- Plant responses to elevated CO2
- Photosynthetic Processes and Mechanisms
- Dam Engineering and Safety
- Plant Reproductive Biology
China Agricultural University
2017-2025
Princess Margaret Cancer Centre
2025
University Health Network
2025
Southern Medical University
2024
Nanjing Tech University
2023-2024
State Key Laboratory of Plant Physiology and Biochemistry
2023
Ocean University of China
2023
State Key Laboratory of Plant Genomics
2020-2022
Second Xiangya Hospital of Central South University
2021
Central South University
2021
Abstract Background DNA methylation is an important epigenetic modification involved in many biological processes. Bisulfite treatment coupled with high-throughput sequencing provides effective approach for studying genome-wide at base resolution. Libraries such as whole genome bisulfite (WGBS) and reduced represented (RRBS) are widely used generating methylomes, demanding efficient versatile tools aligning data. Results We have developed BS-Seeker2, updated version of BS Seeker, a full...
Abstract Tibetan wheat is grown under environmental constraints at high-altitude conditions, but its underlying adaptation mechanism remains unknown. Here, we present a draft genome sequence of semi-wild ( Triticum aestivum ssp. tibetanum Shao) accession Zang1817 and re-sequence 245 accessions, including world-wide landraces, cultivars as well landraces. We demonstrate that environments can trigger extensive reshaping genomes, also uncover accessions accumulate adapted haplotypes related...
In wheat (Triticum aestivum L.), breeding efforts have focused intensively on improving grain yield and quality. For quality, the content composition of seed storage proteins (SSPs) determine elasticity dough flour processing Moreover, starch levels in seeds are associated with yield. However, little is known about mechanisms that coordinate SSP accumulation wheat. this study, we explored role endosperm-specific NAC transcription factor TaNAC019 coordinating accumulation. binds to promoters...
Abstract Modern green revolution varieties of wheat ( Triticum aestivum L.) confer semi-dwarf and lodging-resistant plant architecture owing to the Reduced height-B1b Rht-B1b ) Rht-D1b alleles 1 . However, both are gain-of-function mutant encoding gibberellin signalling repressors that stably repress growth negatively affect nitrogen-use efficiency grain filling 2–5 Therefore, harbouring or usually produce smaller require higher nitrogen fertilizer inputs maintain their yields. Here we...
Abstract Motivation DNA methylation is important for gene silencing and imprinting in both plants animals. Recent advances bisulfite sequencing allow detection of single nucleotide variations (SNVs) achieving high sensitivity, but accurately identifying heterozygous SNVs from partially C-to-T converted sequences remains challenging. Results We designed two methods, BayesWC BinomWC, that substantially improved the precision SNV calls ∼80% to 99% while retaining comparable recalls. With these...
Deletion of the chromatin remodeler chromodomain helicase DNA-binding protein 1 (CHD1) is a common genomic alteration found in human prostate cancers (PCas). CHD1 loss represents distinct PCa subtype characterized by SPOP mutation and higher instability. However, role development vivo its clinical utility remain unclear.To study management, we generated genetically engineered mouse model with prostate-specific deletion murine Chd1 as well isogenic wild-type homozygous deleted benign lines....
In grass crops, leaf angle is determined by development of the lamina joint, tissue connecting blade and sheath, closely related to crop architecture yield. this study, we identified a mutant generated fast neutron radiation that exhibited an erect phenotype caused defects in joint development. Map-based cloning revealed gene
Six subspecies of hexaploid wheat (Triticum aestivum) have been identified, but the origin Indian dwarf sphaerococcum), only with round grains, is currently unknown. Here, we isolated grain-shape gene Tasg-D1 in T. sphaerococcum via positional cloning. encodes a Ser/Thr protein kinase glycogen synthase kinase3 (STKc_GSK3) that negatively regulates brassinosteroid signaling. Expression TaSG-D1 and mutant form Arabidopsis (Arabidopsis thaliana) suggested single amino acid substitution...
Grain yield in bread wheat (Triticum aestivum L.) is largely determined by inflorescence architecture. Zang734 an endemic Tibetan variety that exhibits a rare triple spikelet (TRS) phenotype with significantly increased spikelet/floret number per spike. However, the molecular basis underlying this specific spike morphology completely unknown. Through map-based cloning, causal genes for TRS trait were isolated. Furthermore, using CRISPR/Cas9-based gene mutation, transcriptome sequencing and...
The spikelet number and heading date are two crucial correlated traits for yield in wheat. Here, a quantitative trait locus (QTL) analysis was conducted F8 recombinant inbred lines (RILs) derived from crossing common wheats with different numbers. A total of 15 stable QTL influencing (TSN) (HD) were detected. Notably, FT-D1, well-known flowering time gene wheat, located within the finely mapped interval major on 7DS (QTsn/Hd.cau-7D). causal indel one G third exon FT-D1 significantly...
Major crops are all survivors of domestication bottlenecks. Studies have focused on the genetic loci related to syndrome, while contribution ancient haplotypes remains largely unknown. Here, an ancestral genomic haploblock dissection method is developed and applied a resequencing dataset 386 tetraploid/hexaploid wheat accessions, generating pan-ancestry map. Together with cytoplastic evidences, we reveal that domesticated polyploid emerged from admixture six founder wild emmer lineages,...
Grain size and filling are two key determinants of grain thousand-kernel weight (TKW) crop yield, therefore they have undergone strong selection since cereal was domesticated. Genetic dissection the traits will improve yield potential in crops. A quantitative trait locus significantly associated with wheat TKW detected on chromosome 7AS flanked by a simple sequence repeat marker Wmc17 Chinese 262 mini-core collection genome-wide association study. Combined bulked segregant RNA-sequencing...
Abstract Cold injury is a major environmental stress affecting the growth and yield of crops. Brassinosteroids (BRs) salicylic acid (SA) play important roles in plant cold tolerance. However, whether or how BR signaling interacts with SA pathway response to still unknown. Here, we identified an methyltransferase, TaSAMT1 that converts methyl (MeSA) confers freezing tolerance wheat (Triticum aestivum). overexpression greatly enhanced tolerance, plants accumulating more MeSA less SA, whereas...
Abstract Heat stress threatens global wheat ( Triticum aestivum ) production, causing dramatic yield losses worldwide. Identifying heat tolerance genes and comprehending molecular mechanisms are essential. Here, we identify a gene, TaSG-D1 E286K , in Indian dwarf sphaerococcum ), which encodes an STKc_GSK3 kinase. improves compared to by enhancing phosphorylation stability of downstream target TaPIF4 under condition. Additionally, reveal evolutionary footprints during selective breeding...
Drought is a major global challenge in agriculture that decreases crop production. γ-Aminobutyric acid (GABA) interfaces with drought stress plants; however, mechanistic understanding of the interaction between GABA accumulation and response remains to be established. Here we showed potassium/proton exchanger TaNHX2 functions as positive regulator resistance wheat by mediating cross-talk stomatal aperture accumulation. interacted glutamate decarboxylase TaGAD1, key enzyme synthesizes from...