A5089673067- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Renal and related cancers
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- RNA modifications and cancer
- Prostate Cancer Treatment and Research
- Neonatal Respiratory Health Research
- Genetics and Neurodevelopmental Disorders
- Erythrocyte Function and Pathophysiology
- PARP inhibition in cancer therapy
Broad Center
2014-2017
University of California, San Francisco
2010-2017
Reproductive Science Center
2015
Deletion of the chromatin remodeler chromodomain helicase DNA-binding protein 1 (CHD1) is a common genomic alteration found in human prostate cancers (PCas). CHD1 loss represents distinct PCa subtype characterized by SPOP mutation and higher instability. However, role development vivo its clinical utility remain unclear.To study management, we generated genetically engineered mouse model with prostate-specific deletion murine Chd1 as well isogenic wild-type homozygous deleted benign lines....
The pluripotent mammalian epiblast undergoes unusually fast cell proliferation. This rapid growth is expected to generate a high transcriptional demand, but the underlying mechanisms remain unknown. We show here that chromatin remodeler Chd1 required for output and development of mouse epiblast. Chd1−/− embryos exhibit proliferation defects increased apoptosis, are smaller than controls by E5.5 fail grow, become patterned or gastrulate. Removal p53 allows progression mutants only E7.0-8.0,...
Significance Adult hematopoietic stem and progenitor cells (HSPCs) develop from a small number of specialized endothelial in the embryo. Very little is known about how this process, as endothelial-to-hematopoietic transition, regulated. In paper, we used mouse genetic knockout models to establish Chd1 first chromatin remodeler, our knowledge, shown regulate transition. not required endothelium prior nor blood system after We found that emergence HSPCs involves an increase total nascent...