A5100647204- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Genetic Mapping and Diversity in Plants and Animals
- Forest, Soil, and Plant Ecology in China
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Research in Cotton Cultivation
- Plant responses to water stress
- Chromium effects and bioremediation
- Mitochondrial Function and Pathology
- Transgenic Plants and Applications
- Enzyme Structure and Function
- Molecular Biology Techniques and Applications
- Remote Sensing and Land Use
- Plant pathogens and resistance mechanisms
- Plant biochemistry and biosynthesis
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Adsorption and biosorption for pollutant removal
- Soybean genetics and cultivation
- Endoplasmic Reticulum Stress and Disease
- Plant Micronutrient Interactions and Effects
Central South University
2023-2024
Central South University of Forestry and Technology
2023-2024
Second Affiliated Hospital of Zhejiang University
2024
Henan Agricultural University
2018-2023
Anhui Provincial Hospital
2022-2023
Wuhan University of Technology
2020-2022
Shandong Special Equipment Inspection Institute
2020-2022
Nanning Normal University
2022
Zhejiang University
2020
Shanghai University
2010-2019
Introduction Cereals are an important source of food, feed and fuel with a rapidly increasing global demand. However, cereal seeds contain high levels starch polysaccharides, making the isolation quality RNA extremely difficult. Objective To develop novel method for extracting total from various starch‐ polysaccharides‐rich seeds, such as maize, rice, sorghum wheat. Methodology We developed modified sodium dodecyl sulphate (SDS)/TRIzol method. The combined use Tris buffer (pH 9.0) SDS before...
Genomic imprinting is an epigenetic phenomenon causing parent-of-origin specific differential expression of maternally and paternally inherited alleles. While many imprinted genes have been identified in plants, the functional roles most them are unknown. In this study, we systematically examine requirement expressed (PEGs) during seed development Arabidopsis thaliana. none 15 analyzed peg mutants has qualitative or quantitative abnormalities development, identify three PEGs that establish...
Proline, an important amino acid, accumulates in many plant species. Besides its role cell responses to environmental stresses, the potential biological functions of proline growth and development are unclear. Here, we report cloning functional characterization maize (Zea mays) classic mutant responding1 (pro1) gene. This gene encodes a Δ1-pyrroline-5- carboxylate synthetase that catalyzes biosynthesis from glutamic acid. Loss function Pro1 significantly inhibits decreases accumulation pro1...
Wild soybean (
Myosins are encoded by multigene families and involved in many basic biological processes. However, their functions plants remain poorly understood. Here, we report the functional characterization of maize (Zea mays) opaque1 (o1), which encodes a myosin XI protein. o1 is classic seed mutant with an opaque endosperm phenotype but normal zein protein content. Compared wild type, cells display dilated endoplasmic reticulum (ER) structures increased number smaller, misshapen bodies. The O1 gene...
In maize, a series of seed mutants with starchy endosperm could increase the lysine content by decreased amount zeins, main storage proteins in endosperm. Cloning and characterization these reveal regulatory mechanisms for zeins accumulation maize Opaque7 (o7) is classic mutant large effects on high content. this study, O7 gene was cloned map-based cloning confirmed transgenic functional complementation RNAi. The o7-ref allele has 12-bp in-frame deletion. four-amino-acid deletion caused low...
Mitochondria are semi-autonomous organelles that the powerhouse of cells. Plant mitochondrial RNA editing guided by pentatricopeptide repeat (PPR) proteins is essential for energy production. We identify a maize defective kernel mutant dek36, which produces small and collapsed kernels, leading to embryos and/or seedlings lethality. Seed filling in dek36 drastically impaired, line with defects observed organization endosperm transfer tissue. Positional cloning reveals DEK36, encoding...
Zeins are the major seed storage proteins in maize (Zea mays). They synthesized on endoplasmic reticulum (ER) and deposited into protein bodies. Failure of signal peptide cleavage from zeins can cause an opaque endosperm mature kernel; however, cellular molecular mechanisms responsible for this phenotype not fully understood. In study, we report cloning characterization a novel, semidominant mutant, floury4 (fl4). fl4 is caused by mutated z1A 19-kD α-zein with defective cleavage. Zein bodies...
Cereal storage proteins are major nitrogen sources for humans and livestock. Prolamins the most abundant protein in cereals. They deposited into bodies (PBs) seed endosperm. The inner structure mechanism prolamin PBs is poorly understood. Maize opaque10 (o10) a classic opaque endosperm mutant with misshapen PBs. Through positional cloning, we found that O10 encodes novel cereal-specific PB protein. Its middle domain contains seven-repeat sequence responsible its dimerization. C terminus...
Summary Riboflavin is the precursor of essential cofactors for diverse metabolic processes. Unlike animals, plants can de novo produce riboflavin through an ancestrally conserved pathway, like bacteria and fungi. However, mechanism by which regulates seed development poorly understood. Here, we report a novel maize ( Zea mays L.) opaque mutant o18 , displays increase in lysine accumulation, but impaired endosperm filling embryo development. O18 encodes rate‐limiting bifunctional enzyme...
Tubulin folding cofactors (TFCs) are required for tubulin folding, α/β heterodimer formation, and microtubule (MT) dynamics in yeast mammals. However, the functions of their plant counterparts remain to be characterized. We identified a natural maize crumpled kernel mutant, crk2, which exhibits reductions endosperm cell number size, as well embryo/seedling lethality. Map-based cloning functional complementation confirmed that ZmTFCB is causal mutation. targeted mainly cytosol. It facilitates...
Expansins are unique plant cell wall proteins that possess the ability to induce immediately extension in vitro and expansion vivo. To investigate biological functions of expansins abundant wood-forming tissues, we cloned two expansin genes from differentiating xylem Chinese fir (Cunninghamia lanceolata (Lamb.) Hook). Phylogenetic reconstruction indicated they belong α-expansin (EXPA), named ClEXPA1 ClEXPA2. Expression pattern analysis demonstrated preferentially expressed cambium region....
Ribosome biogenesis is a fundamental cellular process in all cells. Impaired ribosome causes developmental defects; however, its molecular and bases are not fully understood. We cloned gene responsible for maize (Zea mays) small seed mutant, dek* (for defective kernel), found that it encodes export associated1 (ZmReas1). Reas1 an AAA-ATPase controls 60S from the nucleus to cytoplasm after maturation. weak mutant allele with decreased function. In cells, mature subunits reduced cytoplasm, but...
Lymphocytes depend on transmethylation reactions for efficient activation and function. These are primarily catalyzed by <i>S</i>-adenosylmethionine-dependent methyltransferases, which convert <i>S</i>-adenosylmethionine to <i>S</i>-adenosyl-l-homocysteine. <i>S</i>-adenosyl-l-homocysteine is then hydrolyzed hydrolase prevent feedback inhibition of reactions. By impeding hydrolase, a build-up occurs, most intracellular cease. Thus, nontoxic inhibitor this enzyme might be useful...
Prolamins, the major cereal seed storage proteins, are sequestered and accumulated in lumen of endoplasmic reticulum (ER), directly assembled into protein bodies (PBs). The content composition prolamins key determinants for quality texture-related traits grain. Concomitantly, PB-inducing fusion system provides an efficient target to produce therapeutic industrial products plants. However, proteome native PB detailed mechanisms underlying its formation still need be determined. We developed a...
It has previously been demonstrated that miRNA (miR)‑647 exhibits an important role in various cancers, and is aberrantly expressed gastric cancer (GC). However, the exact of miR‑647 GC still remains unclear. The present study aimed to investigate functional significance its target gene GC. TargetScan Miranda databases were used predict putative targets, prediction was validated by Dual‑luciferase Reporter Assays. To whether affects cell behavior, a stable...