A5101604101- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Plant nutrient uptake and metabolism
- Fungal and yeast genetics research
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Chromatin Dynamics
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Rice Cultivation and Yield Improvement
- RNA Research and Splicing
- GABA and Rice Research
- Cholinesterase and Neurodegenerative Diseases
- Genetic Syndromes and Imprinting
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- Peroxisome Proliferator-Activated Receptors
- RNA regulation and disease
- Plant Gene Expression Analysis
Chinese Academy of Sciences
2004-2025
Rockefeller University
2013-2025
Institute of Biophysics
2025
Ocean University of China
2025
ShanghaiTech University
2025
Nanjing University of Chinese Medicine
2020-2024
Jiangsu Province Hospital
2020-2024
University of Colorado Anschutz Medical Campus
2014-2024
University of Colorado Health
2024
Leibniz Institute of Plant Genetics and Crop Plant Research
2020-2024
Summary In Arabidopsis, the tapetum plays important roles in anther development by providing enzymes for callose dissolution and materials pollen‐wall formation, supplying nutrients pollen development. Here, we report identification characterization of a male‐sterile mutant, defective tapetal function 1 ( tdf1 ), that exhibits irregular division dysfunction tapetum. The TDF1 gene was characterized using map‐based cloning strategy, confirmed genetic complementation. It encodes putative R2R3...
Glioblastoma multiforme (GBM) remains a therapeutic challenge due to its aggressive nature and recurrence. This study establishes radioresistant GBM cell model through repeated irradiation observes cellular senescence-like phenotype in these cells. Comprehensive genomic transcriptomic analyses identify IFI16 as central regulator of this contributes radioresistance. activates HMOX1 transcription thereby attenuating ferroptosis by reducing lipid peroxidation, ROS production, intracellular Fe2+...
Abstract DNA polymorphism is the basis to develop molecular markers that are widely used in genetic mapping today. A genome-wide rice (Oryza sativa) database has been constructed this work using genomes of Nipponbare, a cultivar japonica, and 93-11, indica. This contains 1,703,176 single nucleotide polymorphisms (SNPs) 479,406 Insertion/Deletions (InDels), approximately one SNP every 268 bp InDel 953 genome. Both SNPs InDels were experimentally validated. Of 109 randomly selected SNPs, 107...
Genomic imprinting is an epigenetic phenomenon causing parent-of-origin specific differential expression of maternally and paternally inherited alleles. While many imprinted genes have been identified in plants, the functional roles most them are unknown. In this study, we systematically examine requirement expressed (PEGs) during seed development Arabidopsis thaliana. none 15 analyzed peg mutants has qualitative or quantitative abnormalities development, identify three PEGs that establish...
Abstract Polycomb repressive complex 1 (PRC1) plays essential roles in cell fate decisions and development. However, its role cancer is less well understood. Here, we show that RNF2 , encoding RING1B, canonical PRC1 (cPRC1) genes are overexpressed breast cancer. We find cPRC1 complexes functionally associate with ERα pioneer factor FOXA1 ER+ cells, BRD4 triple-negative cells (TNBC). While still exerts function, it also recruited to oncogenic active enhancers. RING1B regulates enhancer...
Molecular genetic analysis is used to characterize the AGT1 gene encoding an α‐glucoside transporter. found in many Saccharomyces cerevisiae laboratory strains and maps a naturally occurring, partially functional allele of MAL1 locus. Agt1p highly hydrophobic, postulated integral membrane protein. It 57% identical Mal61p, maltose permease encoded at MAL6 , also member 12 transmembrane domain superfamily sugar transporters. Like high‐affinity, maltose/proton symporter, but Mal61p capable...
The incidence of obesity is now at epidemic proportions and has resulted in the emergence nonalcoholic fatty liver disease (NAFLD) as a common metabolic disorder that can lead to injury cirrhosis. Excess sucrose long-chain saturated acids diet may play role development progression NAFLD. One factor linking damage dysfunction endoplasmic reticulum (ER). Although there currently no proven, effective therapy for NAFLD, amino sulfonic acid taurine protective against various disturbances,...
Gelatinization temperature (GT) is an important parameter in evaluating the cooking and eating quality of rice. Indeed, phenotype, biochemistry inheritance GT have been widely studied recent times. Previous map-based cloning revealed that was controlled by ALK gene, which encodes a putative soluble starch synthase II-3. Complementation vector RNAi were constructed transformed into Nipponbare mediated Agrobacterium. Phenotypic molecular analyses transgenic lines provided direct evidence for...
Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive others required for the lifecycle. Using differential affinity purifications, quantitative mass spectrometry, next generation RNA sequencing, we have characterized nucleic acids associated distinctive, enzymatically macromolecular complexes. Among...
An infant presented with fatal infantile lactic acidosis and cardiomyopathy, was found to have profoundly decreased activity of respiratory chain complex I in muscle, heart liver. Exome sequencing revealed compound heterozygous mutations NDUFB10, which encodes an accessory subunit located within the PD part I. One mutation resulted a premature stop codon absent protein, while second replaced highly conserved cysteine 107 serine residue. Protein expression NDUFB10 muscle heart, less so liver...
Hua Jiang1, Jordi Moreno-Romero1, Juan Santos-González1, Geert De Jaeger2,3, Kris Gevaert4,5, Eveline Van Slijke2,3 and Claudia Köhler1 1Department of Plant Biology, Uppsala BioCenter, Swedish University Agricultural Sciences, Linnean Center 75007, Sweden; 2Department Biotechnology Bioinformatics, Ghent University, 9052, Belgium; 3VIB for Systems 4Department Biochemistry, 5VIB Medical Biotechnology, Belgium Corresponding author: claudia.kohler{at}slu.se
The sensitivity of myocardium is enhanced to ischemia/reperfusion (I/R) injury under PM2.5 exposure. It still prelude for lncRNA-miRNA pair in the study aggravated myocardial I/R In this study, we first built a rat model 30 min ischemia and 24 h reperfusion followed (6.0 mg/kg) We found exposure could obviously aggravate fields damage, apoptosis levels cardiac function which were evaluated by TTC staining, TUNEL echocardiography, respectively. Then, based on results sequencing RT-qPCR,...
An efficient and reliable procedure for the capture of affinity-tagged proteins associated complexes from human cell lines is reported. Through multiple optimizations, high yield low background affinity-purifications are achieved modest quantities cells expressing endogenous-level tagged proteins. Isolations triple-FLAG GFP-tagged fusion involved in RNA metabolism presented.
Down syndrome (DS) is a genetic disorder caused by trisomy of chromosome 21. Abnormalities in number have the potential to lead disruption proteostasis network (PN) and accumulation misfolded proteins. DS individuals suffer from several comorbidities, we hypothesized that could contribute observed pathology decreased cell viability DS. Our results confirm presence disrupted PN DS, as its elements, including unfolded protein response, chaperone system, proteasomal degradation exhibited...
Long interspersed element-1 (LINE-1, L1) is the major driver of mobile DNA activity in modern humans. When expressed, LINE-1 loci produce bicistronic transcripts encoding two proteins essential for retrotransposition, ORF1p and ORF2p. Many types human cancers are characterized by L1 promoter hypomethylation, transcription, protein expression, somatic retrotransposition. ORF2p encodes endonuclease reverse transcriptase activities required Its expression poorly tissues cell lines.We report...
Abstract The nuclear lamina is a complex network of lamins and lamin-associated membrane proteins, which scaffold the nucleus to maintain structural integrity. In Arabidopsis thaliana , matrix constituent proteins (NMCPs) are essential components required integrity specific perinuclear chromatin anchoring. At periphery, suppressed overlapping with repetitive sequences inactive protein-coding genes enriched. chromosomal level, plant organization in interphase nuclei flexible responds various...
We describe the complete synthesis, assembly, debugging, and characterization of a synthetic 404,963 bp chromosome,
The addition of glucose to maltose-fermenting Saccharomyces cerevisiae cells causes a rapid and irreversible loss the ability transport maltose, resulting both from repression transcription maltose permease gene inactivation permease. latter is referred as glucose-induced or catabolite inactivation. We describe an analysis this process in strain expressing hemagglutinin (HA)-tagged allele MAL61, encoding transfer maltose-induced Mal61/HA protein rich medium containing produces decrease rates...