A5100399654- Immune Response and Inflammation
- Immune cells in cancer
- Immune Cell Function and Interaction
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- interferon and immune responses
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Insect and Arachnid Ecology and Behavior
- NF-κB Signaling Pathways
- Atherosclerosis and Cardiovascular Diseases
- Extracellular vesicles in disease
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Cytokine Signaling Pathways and Interactions
- Research in Cotton Cultivation
- Mycobacterium research and diagnosis
- Ferroptosis and cancer prognosis
- Lipid metabolism and biosynthesis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Drug Solubulity and Delivery Systems
- Inflammatory Biomarkers in Disease Prognosis
Affiliated Hospital of Nantong University
2019-2024
Nantong University
2019-2024
Soochow University
2023-2024
Jilin Agricultural University
2015-2023
Institute of Genomics and Integrative Biology
2023
Harvard University
2023
Pondicherry University
2023
Fudan University
2016-2023
Zhongshan Hospital
2021-2023
L'Oreal (United States)
2023
Placental trophoblasts form the interface between fetal and maternal environments serve to limit maternal–fetal spread of viruses. Here we show that cultured primary human placental are highly resistant infection by a number viruses and, importantly, confer this resistance nonplacental recipient cells exosome-mediated delivery specific microRNAs (miRNAs). We miRNA members chromosome 19 cluster, which almost exclusively expressed in placenta, packaged within trophoblast-derived exosomes...
The CRISPR-Cas system can treat autosomal dominant diseases by nonhomologous end joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide mutations cannot be discriminated from wild-type alleles current systems. Here, we functionally screened six Cas12j nucleases and determined Cas12j-8 as an ideal genome editor with a hypercompact size. displayed comparable activity to AsCas12a Un1Cas12f1. is highly specific nuclease sensitive mismatches in the protospacer adjacent...
Given the rapidly aging population, aging-related diseases are becoming an excessive burden on global healthcare system. Metformin has been shown to be beneficial many age-related disorders, as well increase lifespan in preclinical animal models. During process, kidney function progressively declines. Currently, whether and how metformin protects remains unclear. In this study, among longevity drugs, including metformin, nicotinamide, resveratrol, rapamycin, senolytics, we unexpectedly found...
Understanding supports for cancer stem-like cells in malignant glioma may suggest therapeutic strategies their elimination. Here, we show that the Toll-like receptor TLR9 is elevated (GSC) which it contributes to growth. overexpression regulated by STAT3, required GSC maintenance. Stimulation of with a CpG ligand (CpG ODN) promoted growth, whereas silencing expression abrogated development. CpG-ODN treatment induced Frizzled4-dependent activation JAK2, thereby activating STAT3. Targeted...
In this study, we prepared maize stover biochar and used artificial simulated aging to prepare three types of aged biochar. The effects on the adsorption capacity Pb Cd were studied. It was found that pH, ash, aromaticity decreased compared fresh straw On contrary, polarity, oxygenated functional groups, specific surface area, average pore size increased. sorption behavior Pb2+ Cd2+ can be well explained by quasi-second-order kinetics Langmuir equation. maximum in single metal system CBC-N...
We describe the complete synthesis, assembly, debugging, and characterization of a synthetic 404,963 bp chromosome,
Abstract We have previously reported that Toll-like receptor (TLR) 2 mRNA was induced after infection with Mycobacterium avium. To investigate the molecular basis of TLR2 expression in macrophages, we cloned and analyzed murine putative 5′-proximal promoter. Transient transfection a 326-bp region from nucleotides −294-+32 relative to first transcription start site sufficient induce maximal luciferase activity at basal level M. avium J774A.1 cells. Sequence analysis showed lacked TATA box but...
Abstract To date, much of our knowledge about the signaling networks involved in innate immune response has come from studies using nonphysiologic model systems rather than actual cells. In this study, we used a dual-tagging proteomic strategy to identify components MyD88 signalosome murine macrophages stimulated with lipid A. This approach revealed 16 potential MyD88-interacting partners, one which, flightless I homolog (Fliih) was verified interact and further characterized as negative...
Background: The morbidity and mortality of gastric cancer (GC) remain high worldwide. With the advent human Genome Sequencing Project, circular RNAs (circRNAs) have attracted widespread attention in research due to their stable ring structure. Our aim was identify differentially expressed circRNAs GC explore potential roles diagnosis, treatment prognostic prediction. Methods: Large-scale gene screening performed 3 pairs tissues adjacent non-cancerous using high-throughput sequencing....
To explore the impact of ARGs on prognosis NSCLC, and its correlation with clinicopathological parameters immune microenvironment. Preliminary research biological functions CEBPA in NSCLC. Using consensus clustering analysis to identify molecular subtypes NSCLC patients; employing LASSO regression multivariate Cox select 7 prognostic risk genes construct a model; validating independent factors using forest plot analysis; analyzing microenvironment correlations ESTIMATE ssGSEA; assessing...
Non-small-cell lung cancer (NSCLC) is a major component of and significantly correlated with poor prognosis. N6-methyladenosine (m6A) RNA methylation closely related to the occurrence, progression, prognosis cancer. The potential biological functions mechanisms m6A in immune microenvironment are still unclear. We assessed modification patterns 1326 NSCLC patient samples based on 20 regulators, linking these clusters tumor cell infiltration. m6Ascore was created quantify individual tumors....
The major challenge of recombinant adeno-associated virus (rAAV) vectors is host immunological barriers. Compared to the neutralizing antibody and cytotoxic T lymphocyte response, immune responses induced by unsatisfactory rAAV manufacturing were largely ignored previously. vector production usually requires large amounts plasmid DNAs. DNA are commonly isolated from DH5α bacterial strain, which contains lipopolysaccharide (LPS) contamination. LPS, also named endotoxin, in intractable,...
The 18-mer oligodeoxynucleotides (ODNs) that can inhibit survivin gene expression were selected as a model drug to study hepatic-targeting delivery system. Novel galactosylated polymers (cholesteryloxycarbonylamino) ethylamine-α,β-polyasparthydrazied (CHE-PAHy-Lacs), which target asialoglycoprotein receptor on hepatic parenchymal cells (PC), designed and synthesized non-toxic, non-antigenic non-teratogenic ligands for liposomes. liposomes incorporating different CHE-PAHy-Lacs prepared...
The macrophage is an important early cellular marker related to risk of future rupture atherosclerotic plaques. Two-channel two-photon luminescence (TPL) microscopy combined with optical coherence tomography (OCT) was used detect, and further characterize the distribution aorta-based macrophages using plasmonic gold nanorose as imaging contrast agent.
Osteoporosis is characterized by decreased bone mass, microarchitectural deterioration, and increased fragility. High-fat diet (HFD)-induced obesity also results in loss, which associated with an imbalanced gut microbiome. However, whether HFD-induced or HFD itself promotes osteoclastogenesis consequent loss remains unclear. In this study, we developed (HIO) non-obesity (NO) mouse models to evaluate the effect of on loss. NO mice were defined as body weight within 5% higher lower than that...
Camelina (Camelina sativa L.) is well known for its high unsaturated fatty acid content and great resistance to environmental stress. However, little about the molecular mechanisms of biosynthesis in this annual oilseed crop. To gain greater insight into mechanism, transcriptome profiles seeds at different developmental stages were analyzed by 454 pyrosequencing. Sequencing two normalized libraries produced 831,632 clean reads. A total 32,759 unigenes with an average length 642 bp obtained...
The aim of the present study is to explore impact tet(A) type I variant (tetA-v1) on its fitness effect in Klebsiella pneumoniae. Clinical K. pneumoniae strains were utilized as parental generate carrying only plasmid vector (pBBR1MCS-5) or tetA-v1 recombinant (ptetA-v1). Antimicrobial susceptibility testing was conducted estimate contribution drug resistance. Plasmid stability evaluated by serial passage over 10 consecutive days absence tigecycline. Biological examined through growth curve...
Non-small cell lung cancer (NSCLC) is a highly heterogeneous malignancy with an extremely high mortality rate. Necroptosis programmed death mode mediated by three major mediators, RIPK1, RIPK3, and MLKL, has been shown to play role in various cancers. To date, the effect of necroptosis on NSCLC remains unclear.In The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases, we downloaded transcriptomes adenocarcinoma (LUAD) patients their corresponding clinicopathological...
Camelina sativa (L.) Crantz is an indispensable oilseed crop, and its seeds contain many unsaturated fatty acids. FAD (fatty acid desaturase) regulates the synthesis of In this research, we performed CsFAD gene family analysis identified 24 genes in Camelina, which were unevenly distributed on 14 19 total chromosomes. Phylogenetic showed that includes four subfamilies, supported by conserved structures motifs genes. addition, investigated expression patterns different tissues Camelina. We...
We have previously reported that NF-kappa B and stimulating factor 1 elements within the proximal mouse Toll-like receptor 2 (TLR2) promoter region are required for transcriptional activation of TLR2 expression following infection with Mycobacterium avium. In present study, we found a rapid increase in both DNase I sensitivity restriction enzyme accessibility at occurred M. Increase covering was associated induction mRNA level. Furthermore, did not appear to result from binding B, but rather...
The ability to distinguish macrophage subtypes noninvasively could have diagnostic potential in cancer, atherosclerosis, and diabetes, where polarized M1 M2 macrophages play critical often opposing roles. Current methods rely on tissue biopsy. Optical imaging techniques based light scattering are of interest as they can be translated into biopsy-free strategies. Because mitochondria relatively strong subcellular centers, known enhanced mitochondrial biogenesis compared M1, we hypothesized...